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Optimization of ligninolytic enzyme activity and production rate with Ceriporiopsis subvermispora for application in bioremediation by varying submerged media composition and growth immobilization support.

Babič J, Likozar B, Pavko A - Int J Mol Sci (2012)

Bottom Line: Remazol Brilliant Blue R (RBBR) was decolorized faster and more efficiently than Copper(II)phthalocyanine (CuP) with BWCM (80% and 60%), since Lac played a crucial role.Decolorization of CuP was initially faster than that of RBBR, due to higher MnP activities in OLM.The extent of decolorization after 14 h was 60% for both dyes.

View Article: PubMed Central - PubMed

Affiliation: Chair of Chemical, Biochemical and Environmental Engineering, Faculty of Chemistry and Chemical Technology, University of Ljubljana, Aškerčeva 5, 1000 Ljubljana, Slovenia; E-Mail: janja.babic@fkkt.uni-lj.si.

ABSTRACT
Response surface methodology (central composite design of experiments) was employed to simultaneously optimize enzyme production and productivities of two ligninolytic enzymes produced by Ceriporiopsis subvermispora. Concentrations of glucose, ammonium tartrate and Polysorbate 80 were varied to establish the optimal composition of liquid media (OLM), where the highest experimentally obtained activities and productivities were 41 U L(-1) and 16 U L(-1) day(-1) for laccase (Lac), and 193 U L(-1) and 80 U L(-1) day(-1) for manganese peroxidase (MnP). Considering culture growth in OLM on various types of immobilization support, the best results were obtained with 1 cm beech wood cubes (BWCM). Enzyme activities in culture filtrate were 152 U L(-1) for Lac and 58 U L(-1) for MnP, since the chemical composition of this immobilization material induced higher Lac activity. Lower enzyme activities were obtained with polyurethane foam. Culture filtrates of OLM and BWCM were applied for dye decolorization. Remazol Brilliant Blue R (RBBR) was decolorized faster and more efficiently than Copper(II)phthalocyanine (CuP) with BWCM (80% and 60%), since Lac played a crucial role. Decolorization of CuP was initially faster than that of RBBR, due to higher MnP activities in OLM. The extent of decolorization after 14 h was 60% for both dyes.

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Normalized and absolute optimum settings and OFLac at w = 0.5 for batch experiments at a temperature of 30 °C; the maximal enzyme activity and productivity (OFLac) are to be obtained after 4 days.
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f5-ijms-13-11365: Normalized and absolute optimum settings and OFLac at w = 0.5 for batch experiments at a temperature of 30 °C; the maximal enzyme activity and productivity (OFLac) are to be obtained after 4 days.

Mentions: Examples of initial glucose, ammonium tartrate, and Polysorbate 80 concentrations for the optimal Lac activity and productivity (OFLac) and Lac and MnP activities (OFLac/MnP) are presented in Tables 3–5 and Figures 5 and 6. In all cases the absence of the surfactant should increase both enzyme activity and productivity. The intermediate concentrations of carbon and nitrogen sources corresponded to the maxima exhibited by the response surfaces in Figure 1. The optimization results had to be verified (Experimental Section) to validate if omitting the surfactant in the medium formulation was indeed favourable for enzyme activity and productivity.


Optimization of ligninolytic enzyme activity and production rate with Ceriporiopsis subvermispora for application in bioremediation by varying submerged media composition and growth immobilization support.

Babič J, Likozar B, Pavko A - Int J Mol Sci (2012)

Normalized and absolute optimum settings and OFLac at w = 0.5 for batch experiments at a temperature of 30 °C; the maximal enzyme activity and productivity (OFLac) are to be obtained after 4 days.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3472751&req=5

f5-ijms-13-11365: Normalized and absolute optimum settings and OFLac at w = 0.5 for batch experiments at a temperature of 30 °C; the maximal enzyme activity and productivity (OFLac) are to be obtained after 4 days.
Mentions: Examples of initial glucose, ammonium tartrate, and Polysorbate 80 concentrations for the optimal Lac activity and productivity (OFLac) and Lac and MnP activities (OFLac/MnP) are presented in Tables 3–5 and Figures 5 and 6. In all cases the absence of the surfactant should increase both enzyme activity and productivity. The intermediate concentrations of carbon and nitrogen sources corresponded to the maxima exhibited by the response surfaces in Figure 1. The optimization results had to be verified (Experimental Section) to validate if omitting the surfactant in the medium formulation was indeed favourable for enzyme activity and productivity.

Bottom Line: Remazol Brilliant Blue R (RBBR) was decolorized faster and more efficiently than Copper(II)phthalocyanine (CuP) with BWCM (80% and 60%), since Lac played a crucial role.Decolorization of CuP was initially faster than that of RBBR, due to higher MnP activities in OLM.The extent of decolorization after 14 h was 60% for both dyes.

View Article: PubMed Central - PubMed

Affiliation: Chair of Chemical, Biochemical and Environmental Engineering, Faculty of Chemistry and Chemical Technology, University of Ljubljana, Aškerčeva 5, 1000 Ljubljana, Slovenia; E-Mail: janja.babic@fkkt.uni-lj.si.

ABSTRACT
Response surface methodology (central composite design of experiments) was employed to simultaneously optimize enzyme production and productivities of two ligninolytic enzymes produced by Ceriporiopsis subvermispora. Concentrations of glucose, ammonium tartrate and Polysorbate 80 were varied to establish the optimal composition of liquid media (OLM), where the highest experimentally obtained activities and productivities were 41 U L(-1) and 16 U L(-1) day(-1) for laccase (Lac), and 193 U L(-1) and 80 U L(-1) day(-1) for manganese peroxidase (MnP). Considering culture growth in OLM on various types of immobilization support, the best results were obtained with 1 cm beech wood cubes (BWCM). Enzyme activities in culture filtrate were 152 U L(-1) for Lac and 58 U L(-1) for MnP, since the chemical composition of this immobilization material induced higher Lac activity. Lower enzyme activities were obtained with polyurethane foam. Culture filtrates of OLM and BWCM were applied for dye decolorization. Remazol Brilliant Blue R (RBBR) was decolorized faster and more efficiently than Copper(II)phthalocyanine (CuP) with BWCM (80% and 60%), since Lac played a crucial role. Decolorization of CuP was initially faster than that of RBBR, due to higher MnP activities in OLM. The extent of decolorization after 14 h was 60% for both dyes.

Show MeSH