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Optimization of ligninolytic enzyme activity and production rate with Ceriporiopsis subvermispora for application in bioremediation by varying submerged media composition and growth immobilization support.

Babič J, Likozar B, Pavko A - Int J Mol Sci (2012)

Bottom Line: Remazol Brilliant Blue R (RBBR) was decolorized faster and more efficiently than Copper(II)phthalocyanine (CuP) with BWCM (80% and 60%), since Lac played a crucial role.Decolorization of CuP was initially faster than that of RBBR, due to higher MnP activities in OLM.The extent of decolorization after 14 h was 60% for both dyes.

View Article: PubMed Central - PubMed

Affiliation: Chair of Chemical, Biochemical and Environmental Engineering, Faculty of Chemistry and Chemical Technology, University of Ljubljana, Aškerčeva 5, 1000 Ljubljana, Slovenia; E-Mail: janja.babic@fkkt.uni-lj.si.

ABSTRACT
Response surface methodology (central composite design of experiments) was employed to simultaneously optimize enzyme production and productivities of two ligninolytic enzymes produced by Ceriporiopsis subvermispora. Concentrations of glucose, ammonium tartrate and Polysorbate 80 were varied to establish the optimal composition of liquid media (OLM), where the highest experimentally obtained activities and productivities were 41 U L(-1) and 16 U L(-1) day(-1) for laccase (Lac), and 193 U L(-1) and 80 U L(-1) day(-1) for manganese peroxidase (MnP). Considering culture growth in OLM on various types of immobilization support, the best results were obtained with 1 cm beech wood cubes (BWCM). Enzyme activities in culture filtrate were 152 U L(-1) for Lac and 58 U L(-1) for MnP, since the chemical composition of this immobilization material induced higher Lac activity. Lower enzyme activities were obtained with polyurethane foam. Culture filtrates of OLM and BWCM were applied for dye decolorization. Remazol Brilliant Blue R (RBBR) was decolorized faster and more efficiently than Copper(II)phthalocyanine (CuP) with BWCM (80% and 60%), since Lac played a crucial role. Decolorization of CuP was initially faster than that of RBBR, due to higher MnP activities in OLM. The extent of decolorization after 14 h was 60% for both dyes.

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Graph of optimal normalized highest values of experimentally obtained Lac and MnP activities (y1,Lac and y1,MnP), optimal normalized highest values of experimentally obtained Lac production rate (y2,Lac) and optimal objective function (OFLac, and OFLac/MnP) plotted against the contribution of the maximal enzyme activity divided by its highest experimental value (w).
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f4-ijms-13-11365: Graph of optimal normalized highest values of experimentally obtained Lac and MnP activities (y1,Lac and y1,MnP), optimal normalized highest values of experimentally obtained Lac production rate (y2,Lac) and optimal objective function (OFLac, and OFLac/MnP) plotted against the contribution of the maximal enzyme activity divided by its highest experimental value (w).

Mentions: Figure 4 shows the optimal values of two objective functions, (OFLac) Lac activity and productivity, and (OFLac/MnP) Lac and MnP activities, which were maximized simultaneously, with variable contributions of Lac activity (w) to the objective function. In the first case one may observe that although the overall objective function OFLac assumes its greatest value (1.17) at w = 0 due to high Lac productivity, Lac activity is greatly decreased (only 40% of the highest experimental value). As the contribution to Lac activity in OFLac, w, increases, Lac activity (y1,Lac) first abruptly and then gradually increases towards 1.14, while concurrently Lac productivity (y2,Lac) exhibits a rather small decrease, from 1.17 to 1.00. This would imply that, with the exception of very rare cases when the maximal productivity would be attained regardless of the relatively low activity (w = 0, OFLac = 1.17), continuous systems should preferably be operated in conditions of the highest activity and only slightly decreased productivity (w = 1, OFLac = 1.14), or at least in this vicinity. Of course, another aspect to consider is the concentration of medium components at which distinct levels of activity or productivity may be achieved.


Optimization of ligninolytic enzyme activity and production rate with Ceriporiopsis subvermispora for application in bioremediation by varying submerged media composition and growth immobilization support.

Babič J, Likozar B, Pavko A - Int J Mol Sci (2012)

Graph of optimal normalized highest values of experimentally obtained Lac and MnP activities (y1,Lac and y1,MnP), optimal normalized highest values of experimentally obtained Lac production rate (y2,Lac) and optimal objective function (OFLac, and OFLac/MnP) plotted against the contribution of the maximal enzyme activity divided by its highest experimental value (w).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3472751&req=5

f4-ijms-13-11365: Graph of optimal normalized highest values of experimentally obtained Lac and MnP activities (y1,Lac and y1,MnP), optimal normalized highest values of experimentally obtained Lac production rate (y2,Lac) and optimal objective function (OFLac, and OFLac/MnP) plotted against the contribution of the maximal enzyme activity divided by its highest experimental value (w).
Mentions: Figure 4 shows the optimal values of two objective functions, (OFLac) Lac activity and productivity, and (OFLac/MnP) Lac and MnP activities, which were maximized simultaneously, with variable contributions of Lac activity (w) to the objective function. In the first case one may observe that although the overall objective function OFLac assumes its greatest value (1.17) at w = 0 due to high Lac productivity, Lac activity is greatly decreased (only 40% of the highest experimental value). As the contribution to Lac activity in OFLac, w, increases, Lac activity (y1,Lac) first abruptly and then gradually increases towards 1.14, while concurrently Lac productivity (y2,Lac) exhibits a rather small decrease, from 1.17 to 1.00. This would imply that, with the exception of very rare cases when the maximal productivity would be attained regardless of the relatively low activity (w = 0, OFLac = 1.17), continuous systems should preferably be operated in conditions of the highest activity and only slightly decreased productivity (w = 1, OFLac = 1.14), or at least in this vicinity. Of course, another aspect to consider is the concentration of medium components at which distinct levels of activity or productivity may be achieved.

Bottom Line: Remazol Brilliant Blue R (RBBR) was decolorized faster and more efficiently than Copper(II)phthalocyanine (CuP) with BWCM (80% and 60%), since Lac played a crucial role.Decolorization of CuP was initially faster than that of RBBR, due to higher MnP activities in OLM.The extent of decolorization after 14 h was 60% for both dyes.

View Article: PubMed Central - PubMed

Affiliation: Chair of Chemical, Biochemical and Environmental Engineering, Faculty of Chemistry and Chemical Technology, University of Ljubljana, Aškerčeva 5, 1000 Ljubljana, Slovenia; E-Mail: janja.babic@fkkt.uni-lj.si.

ABSTRACT
Response surface methodology (central composite design of experiments) was employed to simultaneously optimize enzyme production and productivities of two ligninolytic enzymes produced by Ceriporiopsis subvermispora. Concentrations of glucose, ammonium tartrate and Polysorbate 80 were varied to establish the optimal composition of liquid media (OLM), where the highest experimentally obtained activities and productivities were 41 U L(-1) and 16 U L(-1) day(-1) for laccase (Lac), and 193 U L(-1) and 80 U L(-1) day(-1) for manganese peroxidase (MnP). Considering culture growth in OLM on various types of immobilization support, the best results were obtained with 1 cm beech wood cubes (BWCM). Enzyme activities in culture filtrate were 152 U L(-1) for Lac and 58 U L(-1) for MnP, since the chemical composition of this immobilization material induced higher Lac activity. Lower enzyme activities were obtained with polyurethane foam. Culture filtrates of OLM and BWCM were applied for dye decolorization. Remazol Brilliant Blue R (RBBR) was decolorized faster and more efficiently than Copper(II)phthalocyanine (CuP) with BWCM (80% and 60%), since Lac played a crucial role. Decolorization of CuP was initially faster than that of RBBR, due to higher MnP activities in OLM. The extent of decolorization after 14 h was 60% for both dyes.

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