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Optimization of ligninolytic enzyme activity and production rate with Ceriporiopsis subvermispora for application in bioremediation by varying submerged media composition and growth immobilization support.

Babič J, Likozar B, Pavko A - Int J Mol Sci (2012)

Bottom Line: Remazol Brilliant Blue R (RBBR) was decolorized faster and more efficiently than Copper(II)phthalocyanine (CuP) with BWCM (80% and 60%), since Lac played a crucial role.Decolorization of CuP was initially faster than that of RBBR, due to higher MnP activities in OLM.The extent of decolorization after 14 h was 60% for both dyes.

View Article: PubMed Central - PubMed

Affiliation: Chair of Chemical, Biochemical and Environmental Engineering, Faculty of Chemistry and Chemical Technology, University of Ljubljana, Aškerčeva 5, 1000 Ljubljana, Slovenia; E-Mail: janja.babic@fkkt.uni-lj.si.

ABSTRACT
Response surface methodology (central composite design of experiments) was employed to simultaneously optimize enzyme production and productivities of two ligninolytic enzymes produced by Ceriporiopsis subvermispora. Concentrations of glucose, ammonium tartrate and Polysorbate 80 were varied to establish the optimal composition of liquid media (OLM), where the highest experimentally obtained activities and productivities were 41 U L(-1) and 16 U L(-1) day(-1) for laccase (Lac), and 193 U L(-1) and 80 U L(-1) day(-1) for manganese peroxidase (MnP). Considering culture growth in OLM on various types of immobilization support, the best results were obtained with 1 cm beech wood cubes (BWCM). Enzyme activities in culture filtrate were 152 U L(-1) for Lac and 58 U L(-1) for MnP, since the chemical composition of this immobilization material induced higher Lac activity. Lower enzyme activities were obtained with polyurethane foam. Culture filtrates of OLM and BWCM were applied for dye decolorization. Remazol Brilliant Blue R (RBBR) was decolorized faster and more efficiently than Copper(II)phthalocyanine (CuP) with BWCM (80% and 60%), since Lac played a crucial role. Decolorization of CuP was initially faster than that of RBBR, due to higher MnP activities in OLM. The extent of decolorization after 14 h was 60% for both dyes.

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(a) Standardized Pareto chart for the maximal Lac activity, [ELac]; and (b) its maximal production rate, d[ELac]/dt for batch experiments at a temperature of 30 °C; x1 is the initial glucose concentration; x2 is the initial ammonium tartrate concentration and x3 is the initial Polysorbate 80 concentration. Dotted line represents the 95% confidence interval margin.
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f3-ijms-13-11365: (a) Standardized Pareto chart for the maximal Lac activity, [ELac]; and (b) its maximal production rate, d[ELac]/dt for batch experiments at a temperature of 30 °C; x1 is the initial glucose concentration; x2 is the initial ammonium tartrate concentration and x3 is the initial Polysorbate 80 concentration. Dotted line represents the 95% confidence interval margin.

Mentions: Subsequently, sensitivity analysis was performed on the quadratic model utilized and its corresponding parameters (the parameters in Equation 2 and Table 2), estimated by linear regression. Cohen’s f2 was used to measure the effect size of individual contributions in the model (x1, the initial glucose, x2, ammonium tartrate, and x3, Polysorbate 80 concentration, y1, y2, y3, and y3 dependent variables), while the F-test was used as a statistical test to determine which effects were within the 95% confidence interval margin (dotted line in Figure 3).


Optimization of ligninolytic enzyme activity and production rate with Ceriporiopsis subvermispora for application in bioremediation by varying submerged media composition and growth immobilization support.

Babič J, Likozar B, Pavko A - Int J Mol Sci (2012)

(a) Standardized Pareto chart for the maximal Lac activity, [ELac]; and (b) its maximal production rate, d[ELac]/dt for batch experiments at a temperature of 30 °C; x1 is the initial glucose concentration; x2 is the initial ammonium tartrate concentration and x3 is the initial Polysorbate 80 concentration. Dotted line represents the 95% confidence interval margin.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3472751&req=5

f3-ijms-13-11365: (a) Standardized Pareto chart for the maximal Lac activity, [ELac]; and (b) its maximal production rate, d[ELac]/dt for batch experiments at a temperature of 30 °C; x1 is the initial glucose concentration; x2 is the initial ammonium tartrate concentration and x3 is the initial Polysorbate 80 concentration. Dotted line represents the 95% confidence interval margin.
Mentions: Subsequently, sensitivity analysis was performed on the quadratic model utilized and its corresponding parameters (the parameters in Equation 2 and Table 2), estimated by linear regression. Cohen’s f2 was used to measure the effect size of individual contributions in the model (x1, the initial glucose, x2, ammonium tartrate, and x3, Polysorbate 80 concentration, y1, y2, y3, and y3 dependent variables), while the F-test was used as a statistical test to determine which effects were within the 95% confidence interval margin (dotted line in Figure 3).

Bottom Line: Remazol Brilliant Blue R (RBBR) was decolorized faster and more efficiently than Copper(II)phthalocyanine (CuP) with BWCM (80% and 60%), since Lac played a crucial role.Decolorization of CuP was initially faster than that of RBBR, due to higher MnP activities in OLM.The extent of decolorization after 14 h was 60% for both dyes.

View Article: PubMed Central - PubMed

Affiliation: Chair of Chemical, Biochemical and Environmental Engineering, Faculty of Chemistry and Chemical Technology, University of Ljubljana, Aškerčeva 5, 1000 Ljubljana, Slovenia; E-Mail: janja.babic@fkkt.uni-lj.si.

ABSTRACT
Response surface methodology (central composite design of experiments) was employed to simultaneously optimize enzyme production and productivities of two ligninolytic enzymes produced by Ceriporiopsis subvermispora. Concentrations of glucose, ammonium tartrate and Polysorbate 80 were varied to establish the optimal composition of liquid media (OLM), where the highest experimentally obtained activities and productivities were 41 U L(-1) and 16 U L(-1) day(-1) for laccase (Lac), and 193 U L(-1) and 80 U L(-1) day(-1) for manganese peroxidase (MnP). Considering culture growth in OLM on various types of immobilization support, the best results were obtained with 1 cm beech wood cubes (BWCM). Enzyme activities in culture filtrate were 152 U L(-1) for Lac and 58 U L(-1) for MnP, since the chemical composition of this immobilization material induced higher Lac activity. Lower enzyme activities were obtained with polyurethane foam. Culture filtrates of OLM and BWCM were applied for dye decolorization. Remazol Brilliant Blue R (RBBR) was decolorized faster and more efficiently than Copper(II)phthalocyanine (CuP) with BWCM (80% and 60%), since Lac played a crucial role. Decolorization of CuP was initially faster than that of RBBR, due to higher MnP activities in OLM. The extent of decolorization after 14 h was 60% for both dyes.

Show MeSH