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Optimization of ligninolytic enzyme activity and production rate with Ceriporiopsis subvermispora for application in bioremediation by varying submerged media composition and growth immobilization support.

Babič J, Likozar B, Pavko A - Int J Mol Sci (2012)

Bottom Line: Remazol Brilliant Blue R (RBBR) was decolorized faster and more efficiently than Copper(II)phthalocyanine (CuP) with BWCM (80% and 60%), since Lac played a crucial role.Decolorization of CuP was initially faster than that of RBBR, due to higher MnP activities in OLM.The extent of decolorization after 14 h was 60% for both dyes.

View Article: PubMed Central - PubMed

Affiliation: Chair of Chemical, Biochemical and Environmental Engineering, Faculty of Chemistry and Chemical Technology, University of Ljubljana, Aškerčeva 5, 1000 Ljubljana, Slovenia; E-Mail: janja.babic@fkkt.uni-lj.si.

ABSTRACT
Response surface methodology (central composite design of experiments) was employed to simultaneously optimize enzyme production and productivities of two ligninolytic enzymes produced by Ceriporiopsis subvermispora. Concentrations of glucose, ammonium tartrate and Polysorbate 80 were varied to establish the optimal composition of liquid media (OLM), where the highest experimentally obtained activities and productivities were 41 U L(-1) and 16 U L(-1) day(-1) for laccase (Lac), and 193 U L(-1) and 80 U L(-1) day(-1) for manganese peroxidase (MnP). Considering culture growth in OLM on various types of immobilization support, the best results were obtained with 1 cm beech wood cubes (BWCM). Enzyme activities in culture filtrate were 152 U L(-1) for Lac and 58 U L(-1) for MnP, since the chemical composition of this immobilization material induced higher Lac activity. Lower enzyme activities were obtained with polyurethane foam. Culture filtrates of OLM and BWCM were applied for dye decolorization. Remazol Brilliant Blue R (RBBR) was decolorized faster and more efficiently than Copper(II)phthalocyanine (CuP) with BWCM (80% and 60%), since Lac played a crucial role. Decolorization of CuP was initially faster than that of RBBR, due to higher MnP activities in OLM. The extent of decolorization after 14 h was 60% for both dyes.

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Schematic diagram of the RSM procedure.
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f10-ijms-13-11365: Schematic diagram of the RSM procedure.

Mentions: To optimize the medium composition with regard to Lac and MnP activity and their productivity, surface response methodology for enzyme activity and productivity was utilized. Central composite design (CCD) (orthogonal) was used applying full factorial, the inscribed type of design, and no limitations of the maximal block size. Although CCD is a really useful approach for the optimization of enzyme production processes it cannot always be successfully applied using fungi; to overcome this possible problem we repeated in triplicate the experiment of the CCD. Modelling, sensitivity analysis and optimization was performed with Matlab R2011a software. The coded values in the central composite design matrix were transformed into real values using minimal and maximal concentration values of 1.0 g L−1 and 10.0 g L−1 for glucose, 0.1 g L−1 and 2.0 g L−1 for ammonium tartrate, and 0.1 g L−1 and 1.0 g L−1 for Polysorbate 80, the real values being presented in Table 1. A schematic diagram of the RSM procedure is laid out in Figure 10, its main steps being response surface design, experiment, modelling, sensitivity analysis, optimization and verification [8–14].


Optimization of ligninolytic enzyme activity and production rate with Ceriporiopsis subvermispora for application in bioremediation by varying submerged media composition and growth immobilization support.

Babič J, Likozar B, Pavko A - Int J Mol Sci (2012)

Schematic diagram of the RSM procedure.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3472751&req=5

f10-ijms-13-11365: Schematic diagram of the RSM procedure.
Mentions: To optimize the medium composition with regard to Lac and MnP activity and their productivity, surface response methodology for enzyme activity and productivity was utilized. Central composite design (CCD) (orthogonal) was used applying full factorial, the inscribed type of design, and no limitations of the maximal block size. Although CCD is a really useful approach for the optimization of enzyme production processes it cannot always be successfully applied using fungi; to overcome this possible problem we repeated in triplicate the experiment of the CCD. Modelling, sensitivity analysis and optimization was performed with Matlab R2011a software. The coded values in the central composite design matrix were transformed into real values using minimal and maximal concentration values of 1.0 g L−1 and 10.0 g L−1 for glucose, 0.1 g L−1 and 2.0 g L−1 for ammonium tartrate, and 0.1 g L−1 and 1.0 g L−1 for Polysorbate 80, the real values being presented in Table 1. A schematic diagram of the RSM procedure is laid out in Figure 10, its main steps being response surface design, experiment, modelling, sensitivity analysis, optimization and verification [8–14].

Bottom Line: Remazol Brilliant Blue R (RBBR) was decolorized faster and more efficiently than Copper(II)phthalocyanine (CuP) with BWCM (80% and 60%), since Lac played a crucial role.Decolorization of CuP was initially faster than that of RBBR, due to higher MnP activities in OLM.The extent of decolorization after 14 h was 60% for both dyes.

View Article: PubMed Central - PubMed

Affiliation: Chair of Chemical, Biochemical and Environmental Engineering, Faculty of Chemistry and Chemical Technology, University of Ljubljana, Aškerčeva 5, 1000 Ljubljana, Slovenia; E-Mail: janja.babic@fkkt.uni-lj.si.

ABSTRACT
Response surface methodology (central composite design of experiments) was employed to simultaneously optimize enzyme production and productivities of two ligninolytic enzymes produced by Ceriporiopsis subvermispora. Concentrations of glucose, ammonium tartrate and Polysorbate 80 were varied to establish the optimal composition of liquid media (OLM), where the highest experimentally obtained activities and productivities were 41 U L(-1) and 16 U L(-1) day(-1) for laccase (Lac), and 193 U L(-1) and 80 U L(-1) day(-1) for manganese peroxidase (MnP). Considering culture growth in OLM on various types of immobilization support, the best results were obtained with 1 cm beech wood cubes (BWCM). Enzyme activities in culture filtrate were 152 U L(-1) for Lac and 58 U L(-1) for MnP, since the chemical composition of this immobilization material induced higher Lac activity. Lower enzyme activities were obtained with polyurethane foam. Culture filtrates of OLM and BWCM were applied for dye decolorization. Remazol Brilliant Blue R (RBBR) was decolorized faster and more efficiently than Copper(II)phthalocyanine (CuP) with BWCM (80% and 60%), since Lac played a crucial role. Decolorization of CuP was initially faster than that of RBBR, due to higher MnP activities in OLM. The extent of decolorization after 14 h was 60% for both dyes.

Show MeSH