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Optimization of ligninolytic enzyme activity and production rate with Ceriporiopsis subvermispora for application in bioremediation by varying submerged media composition and growth immobilization support.

Babič J, Likozar B, Pavko A - Int J Mol Sci (2012)

Bottom Line: Remazol Brilliant Blue R (RBBR) was decolorized faster and more efficiently than Copper(II)phthalocyanine (CuP) with BWCM (80% and 60%), since Lac played a crucial role.Decolorization of CuP was initially faster than that of RBBR, due to higher MnP activities in OLM.The extent of decolorization after 14 h was 60% for both dyes.

View Article: PubMed Central - PubMed

Affiliation: Chair of Chemical, Biochemical and Environmental Engineering, Faculty of Chemistry and Chemical Technology, University of Ljubljana, Aškerčeva 5, 1000 Ljubljana, Slovenia; E-Mail: janja.babic@fkkt.uni-lj.si.

ABSTRACT
Response surface methodology (central composite design of experiments) was employed to simultaneously optimize enzyme production and productivities of two ligninolytic enzymes produced by Ceriporiopsis subvermispora. Concentrations of glucose, ammonium tartrate and Polysorbate 80 were varied to establish the optimal composition of liquid media (OLM), where the highest experimentally obtained activities and productivities were 41 U L(-1) and 16 U L(-1) day(-1) for laccase (Lac), and 193 U L(-1) and 80 U L(-1) day(-1) for manganese peroxidase (MnP). Considering culture growth in OLM on various types of immobilization support, the best results were obtained with 1 cm beech wood cubes (BWCM). Enzyme activities in culture filtrate were 152 U L(-1) for Lac and 58 U L(-1) for MnP, since the chemical composition of this immobilization material induced higher Lac activity. Lower enzyme activities were obtained with polyurethane foam. Culture filtrates of OLM and BWCM were applied for dye decolorization. Remazol Brilliant Blue R (RBBR) was decolorized faster and more efficiently than Copper(II)phthalocyanine (CuP) with BWCM (80% and 60%), since Lac played a crucial role. Decolorization of CuP was initially faster than that of RBBR, due to higher MnP activities in OLM. The extent of decolorization after 14 h was 60% for both dyes.

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Estimated response surface for OFLac (the contribution of relative laccase (Lac) activity to OFLac (w) was set at (a–c) 0.25, (d–f) 0.50 and (g–i) 0.75), on varying the initial glucose (x1), ammonium tartrate (x2) and Polysorbate 80 (x3) concentrations for batch experiments at a temperature of 30 °C.
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f1-ijms-13-11365: Estimated response surface for OFLac (the contribution of relative laccase (Lac) activity to OFLac (w) was set at (a–c) 0.25, (d–f) 0.50 and (g–i) 0.75), on varying the initial glucose (x1), ammonium tartrate (x2) and Polysorbate 80 (x3) concentrations for batch experiments at a temperature of 30 °C.

Mentions: In order not to introduce too many variables to the design of experiments, as the three crucial independent variables, pertinent to enzyme production, two principal nutrients (carbon and nitrogen source—glucose (1) and ammonium tartrate (2), respectively) and Polysorbate 80 (3), a non-ionic surfactant and emulsifier, were chosen. In Figure 1, the influence of medium components specifically; glucose (x1), ammonium tartrate (x2) and Polysorbate 80 (x3), on the combined activity and productivity of Lac (OFLac) is presented. In Figure 1a–c the Lac activity has a lower contribution (0.25) to the overall objective function (Equation 1) than the Lac productivity (0.75), whereas its impact gradually increases through Figure 1d–f (w = 0.50) and 1g–i (w = 0.75).


Optimization of ligninolytic enzyme activity and production rate with Ceriporiopsis subvermispora for application in bioremediation by varying submerged media composition and growth immobilization support.

Babič J, Likozar B, Pavko A - Int J Mol Sci (2012)

Estimated response surface for OFLac (the contribution of relative laccase (Lac) activity to OFLac (w) was set at (a–c) 0.25, (d–f) 0.50 and (g–i) 0.75), on varying the initial glucose (x1), ammonium tartrate (x2) and Polysorbate 80 (x3) concentrations for batch experiments at a temperature of 30 °C.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3472751&req=5

f1-ijms-13-11365: Estimated response surface for OFLac (the contribution of relative laccase (Lac) activity to OFLac (w) was set at (a–c) 0.25, (d–f) 0.50 and (g–i) 0.75), on varying the initial glucose (x1), ammonium tartrate (x2) and Polysorbate 80 (x3) concentrations for batch experiments at a temperature of 30 °C.
Mentions: In order not to introduce too many variables to the design of experiments, as the three crucial independent variables, pertinent to enzyme production, two principal nutrients (carbon and nitrogen source—glucose (1) and ammonium tartrate (2), respectively) and Polysorbate 80 (3), a non-ionic surfactant and emulsifier, were chosen. In Figure 1, the influence of medium components specifically; glucose (x1), ammonium tartrate (x2) and Polysorbate 80 (x3), on the combined activity and productivity of Lac (OFLac) is presented. In Figure 1a–c the Lac activity has a lower contribution (0.25) to the overall objective function (Equation 1) than the Lac productivity (0.75), whereas its impact gradually increases through Figure 1d–f (w = 0.50) and 1g–i (w = 0.75).

Bottom Line: Remazol Brilliant Blue R (RBBR) was decolorized faster and more efficiently than Copper(II)phthalocyanine (CuP) with BWCM (80% and 60%), since Lac played a crucial role.Decolorization of CuP was initially faster than that of RBBR, due to higher MnP activities in OLM.The extent of decolorization after 14 h was 60% for both dyes.

View Article: PubMed Central - PubMed

Affiliation: Chair of Chemical, Biochemical and Environmental Engineering, Faculty of Chemistry and Chemical Technology, University of Ljubljana, Aškerčeva 5, 1000 Ljubljana, Slovenia; E-Mail: janja.babic@fkkt.uni-lj.si.

ABSTRACT
Response surface methodology (central composite design of experiments) was employed to simultaneously optimize enzyme production and productivities of two ligninolytic enzymes produced by Ceriporiopsis subvermispora. Concentrations of glucose, ammonium tartrate and Polysorbate 80 were varied to establish the optimal composition of liquid media (OLM), where the highest experimentally obtained activities and productivities were 41 U L(-1) and 16 U L(-1) day(-1) for laccase (Lac), and 193 U L(-1) and 80 U L(-1) day(-1) for manganese peroxidase (MnP). Considering culture growth in OLM on various types of immobilization support, the best results were obtained with 1 cm beech wood cubes (BWCM). Enzyme activities in culture filtrate were 152 U L(-1) for Lac and 58 U L(-1) for MnP, since the chemical composition of this immobilization material induced higher Lac activity. Lower enzyme activities were obtained with polyurethane foam. Culture filtrates of OLM and BWCM were applied for dye decolorization. Remazol Brilliant Blue R (RBBR) was decolorized faster and more efficiently than Copper(II)phthalocyanine (CuP) with BWCM (80% and 60%), since Lac played a crucial role. Decolorization of CuP was initially faster than that of RBBR, due to higher MnP activities in OLM. The extent of decolorization after 14 h was 60% for both dyes.

Show MeSH