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Phenolic compounds from Halimodendron halodendron (Pall.) voss and their antimicrobial and antioxidant activities.

Wang J, Lou J, Luo C, Zhou L, Wang M, Wang L - Int J Mol Sci (2012)

Bottom Line: All these phenolic compounds showed significant antimicrobial activity with a broad spectrum as well as antioxidant activity based on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and β-carotene-linoleic acid bleaching assays.In general, the flavonol aglycones with relatively low polarity exhibited stronger activities than the glycosides.The results suggest the potential of this plant as a source of functional food ingredients and provide support data for its utilization as forage as well.

View Article: PubMed Central - PubMed

Affiliation: College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China; E-Mails: nxwjh8121@sina.com (J.W.); azaazafighting88@163.com (J.L.); luochaobest@163.com (C. L.).

ABSTRACT
Halimodendron halodendron has been used as forage in northwestern China for a long time. Its young leaves and flowers are edible and favored by indigenous people. In this study, eleven phenolic compounds were bioassay-guided and isolated from the aerial parts of H. halodendron for the first time. They were identified by means of physicochemical and spectrometric analysis as quercetin (1), 3,5,7,8,4'-pentahydroxy-3'-methoxy flavone (2), 3-O-methylquercetin (3), 3,3'-di-O-methylquercetin (4), 3,3'-di-O-methylquercetin-7-O-β-d-glucopyranoside (5), isorhamentin-3-O-β-d-rutinoside (6), 8-O-methylretusin (7), 8-O-methylretusin-7-O-β-d-glucopyranoside (8), salicylic acid (9), p-hydroxybenzoic acid (ferulic acid) (10), and 4-hydroxy-3-methoxy cinnamic acid (11). They were sorted as flavonols (1-6), soflavones (7 and 8), and phenolic acids (9-11). Among the compounds, flanools 1-4 revealed a strong antibacterial activity with minimum inhibitory concentration (MIC) values of 50-150 μg/mL, and median inhibitory concentration (IC(50)) values of 26.8-125.1 μg/mL. The two isoflavones (7 and 8) showed moderate inhibitory activity on the test bacteria. Three phenolic acids (9, 10 and 11) showed strong antibacterial activity with IC(50) values of 28.1-149.7 μg/mL. Antifungal activities of the compounds were similar to their antibacterial activities. All these phenolic compounds showed significant antimicrobial activity with a broad spectrum as well as antioxidant activity based on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and β-carotene-linoleic acid bleaching assays. In general, the flavonol aglycones with relatively low polarity exhibited stronger activities than the glycosides. The results suggest the potential of this plant as a source of functional food ingredients and provide support data for its utilization as forage as well.

Show MeSH
Minimum inhibitory concentration (MIC) (A) and median inhibitory concentration (IC50) (B) of the phenolic compounds from H. halodendron on the test fungi. The positive controls (CK+) for Candida albicans and Magnaporthe oryzae were amphotericin B and carbendazim, respectively.
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f3-ijms-13-11349: Minimum inhibitory concentration (MIC) (A) and median inhibitory concentration (IC50) (B) of the phenolic compounds from H. halodendron on the test fungi. The positive controls (CK+) for Candida albicans and Magnaporthe oryzae were amphotericin B and carbendazim, respectively.

Mentions: The antifungal activity of the phenolic compounds with their MIC and IC50 values shown in Figure 3 was similar to the antibacterial activity. Compounds 1, 3, 4 and 7 had the highest inhibitory activities on M. oryzae spore germination with the lowest IC50 values of 35.80–67.90 μg/mL. Glycosides 5, 6 and 8 only exhibited weak activity with relatively high IC50 values of 182.9–264.7 μg/mL. The three phenolic acids showed moderate inhibitory activity with IC50 values of 99.1–167.5 μg/mL. All tested phenolic compounds exhibited moderate antifungal activity on Candida albicans, most of which were even stronger than the positive control amphotericin B, except for Compounds 5 and 6 showing no activity. The results suggest that the fungus Candida albicans is an amphotericin-resistant strain and these phenolic compounds are potential antifungal agents against amphotericin-resistant C. albicans.


Phenolic compounds from Halimodendron halodendron (Pall.) voss and their antimicrobial and antioxidant activities.

Wang J, Lou J, Luo C, Zhou L, Wang M, Wang L - Int J Mol Sci (2012)

Minimum inhibitory concentration (MIC) (A) and median inhibitory concentration (IC50) (B) of the phenolic compounds from H. halodendron on the test fungi. The positive controls (CK+) for Candida albicans and Magnaporthe oryzae were amphotericin B and carbendazim, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3472750&req=5

f3-ijms-13-11349: Minimum inhibitory concentration (MIC) (A) and median inhibitory concentration (IC50) (B) of the phenolic compounds from H. halodendron on the test fungi. The positive controls (CK+) for Candida albicans and Magnaporthe oryzae were amphotericin B and carbendazim, respectively.
Mentions: The antifungal activity of the phenolic compounds with their MIC and IC50 values shown in Figure 3 was similar to the antibacterial activity. Compounds 1, 3, 4 and 7 had the highest inhibitory activities on M. oryzae spore germination with the lowest IC50 values of 35.80–67.90 μg/mL. Glycosides 5, 6 and 8 only exhibited weak activity with relatively high IC50 values of 182.9–264.7 μg/mL. The three phenolic acids showed moderate inhibitory activity with IC50 values of 99.1–167.5 μg/mL. All tested phenolic compounds exhibited moderate antifungal activity on Candida albicans, most of which were even stronger than the positive control amphotericin B, except for Compounds 5 and 6 showing no activity. The results suggest that the fungus Candida albicans is an amphotericin-resistant strain and these phenolic compounds are potential antifungal agents against amphotericin-resistant C. albicans.

Bottom Line: All these phenolic compounds showed significant antimicrobial activity with a broad spectrum as well as antioxidant activity based on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and β-carotene-linoleic acid bleaching assays.In general, the flavonol aglycones with relatively low polarity exhibited stronger activities than the glycosides.The results suggest the potential of this plant as a source of functional food ingredients and provide support data for its utilization as forage as well.

View Article: PubMed Central - PubMed

Affiliation: College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China; E-Mails: nxwjh8121@sina.com (J.W.); azaazafighting88@163.com (J.L.); luochaobest@163.com (C. L.).

ABSTRACT
Halimodendron halodendron has been used as forage in northwestern China for a long time. Its young leaves and flowers are edible and favored by indigenous people. In this study, eleven phenolic compounds were bioassay-guided and isolated from the aerial parts of H. halodendron for the first time. They were identified by means of physicochemical and spectrometric analysis as quercetin (1), 3,5,7,8,4'-pentahydroxy-3'-methoxy flavone (2), 3-O-methylquercetin (3), 3,3'-di-O-methylquercetin (4), 3,3'-di-O-methylquercetin-7-O-β-d-glucopyranoside (5), isorhamentin-3-O-β-d-rutinoside (6), 8-O-methylretusin (7), 8-O-methylretusin-7-O-β-d-glucopyranoside (8), salicylic acid (9), p-hydroxybenzoic acid (ferulic acid) (10), and 4-hydroxy-3-methoxy cinnamic acid (11). They were sorted as flavonols (1-6), soflavones (7 and 8), and phenolic acids (9-11). Among the compounds, flanools 1-4 revealed a strong antibacterial activity with minimum inhibitory concentration (MIC) values of 50-150 μg/mL, and median inhibitory concentration (IC(50)) values of 26.8-125.1 μg/mL. The two isoflavones (7 and 8) showed moderate inhibitory activity on the test bacteria. Three phenolic acids (9, 10 and 11) showed strong antibacterial activity with IC(50) values of 28.1-149.7 μg/mL. Antifungal activities of the compounds were similar to their antibacterial activities. All these phenolic compounds showed significant antimicrobial activity with a broad spectrum as well as antioxidant activity based on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and β-carotene-linoleic acid bleaching assays. In general, the flavonol aglycones with relatively low polarity exhibited stronger activities than the glycosides. The results suggest the potential of this plant as a source of functional food ingredients and provide support data for its utilization as forage as well.

Show MeSH