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Determination and analysis of ustiloxins A and B by LC-ESI-MS and HPLC in false smut balls of rice.

Shan T, Sun W, Liu H, Gao S, Lu S, Wang M, Sun W, Chen Z, Wang S, Zhou L - Int J Mol Sci (2012)

Bottom Line: Ustiloxins are cyclopeptide mycotoxins produced by the pathogenic fungus Villosiclava virens of rice false smut.Ustiloxins A and B as two main mycotoxins were determined conveniently by LC-ESI-MS in the water extract from rice false smut balls which were mostly composed of the chlamydospores and mycelia of the pathogen.This is the first report on the determination and analysis of ustiloxins A and B simultaneously by LC-ESI-MS and HPLC in false smut balls of rice.

View Article: PubMed Central - PubMed

Affiliation: College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China; E-Mails: shan5400388@163.com (T.S.); sunweibo.1001@163.com (W.S.); hardyliuhao@163.com (H.L.); gaoshankonglong@163.com (S.G.); shiqionglu@126.com (S.L.); wangma@cau.edu.cn (M.W.); wxs@cau.edu.cn (W.S.).

ABSTRACT
Ustiloxins are cyclopeptide mycotoxins produced by the pathogenic fungus Villosiclava virens of rice false smut. Ustiloxins A and B as two main mycotoxins were determined conveniently by LC-ESI-MS in the water extract from rice false smut balls which were mostly composed of the chlamydospores and mycelia of the pathogen. Both ustiloxins A and B in the water extract were also quantitatively analyzed by HPLC. This is the first report on the determination and analysis of ustiloxins A and B simultaneously by LC-ESI-MS and HPLC in false smut balls of rice.

Show MeSH
HPLC-UV analysis of ustiloxins A and B in rice false smut balls. (A) is the HPLC profile of the water extract; (B) is the HPLC profile of the reference ustiloxins A and B. Figures 4C and 4D are the UV absorption spectra of ustiloxins A and B, respectively.
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f4-ijms-13-11275: HPLC-UV analysis of ustiloxins A and B in rice false smut balls. (A) is the HPLC profile of the water extract; (B) is the HPLC profile of the reference ustiloxins A and B. Figures 4C and 4D are the UV absorption spectra of ustiloxins A and B, respectively.

Mentions: A rapid HPLC method was developed for analysis of ustiloxins A and B in rice false smut balls. Figure 4A,B show the HPLC-UV profiles of the water extract and reference ustiloxins A and B, respectively. Both ustiloxins A and B in rice false smut balls were identified by comparison of their retention times with the reference ustiloxins as well as their UV absorption spectra (Figure 4C for ustiloxin A, and Figure 4D for ustiloxin B). HPLC analysis was complete in 20 min.


Determination and analysis of ustiloxins A and B by LC-ESI-MS and HPLC in false smut balls of rice.

Shan T, Sun W, Liu H, Gao S, Lu S, Wang M, Sun W, Chen Z, Wang S, Zhou L - Int J Mol Sci (2012)

HPLC-UV analysis of ustiloxins A and B in rice false smut balls. (A) is the HPLC profile of the water extract; (B) is the HPLC profile of the reference ustiloxins A and B. Figures 4C and 4D are the UV absorption spectra of ustiloxins A and B, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3472744&req=5

f4-ijms-13-11275: HPLC-UV analysis of ustiloxins A and B in rice false smut balls. (A) is the HPLC profile of the water extract; (B) is the HPLC profile of the reference ustiloxins A and B. Figures 4C and 4D are the UV absorption spectra of ustiloxins A and B, respectively.
Mentions: A rapid HPLC method was developed for analysis of ustiloxins A and B in rice false smut balls. Figure 4A,B show the HPLC-UV profiles of the water extract and reference ustiloxins A and B, respectively. Both ustiloxins A and B in rice false smut balls were identified by comparison of their retention times with the reference ustiloxins as well as their UV absorption spectra (Figure 4C for ustiloxin A, and Figure 4D for ustiloxin B). HPLC analysis was complete in 20 min.

Bottom Line: Ustiloxins are cyclopeptide mycotoxins produced by the pathogenic fungus Villosiclava virens of rice false smut.Ustiloxins A and B as two main mycotoxins were determined conveniently by LC-ESI-MS in the water extract from rice false smut balls which were mostly composed of the chlamydospores and mycelia of the pathogen.This is the first report on the determination and analysis of ustiloxins A and B simultaneously by LC-ESI-MS and HPLC in false smut balls of rice.

View Article: PubMed Central - PubMed

Affiliation: College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China; E-Mails: shan5400388@163.com (T.S.); sunweibo.1001@163.com (W.S.); hardyliuhao@163.com (H.L.); gaoshankonglong@163.com (S.G.); shiqionglu@126.com (S.L.); wangma@cau.edu.cn (M.W.); wxs@cau.edu.cn (W.S.).

ABSTRACT
Ustiloxins are cyclopeptide mycotoxins produced by the pathogenic fungus Villosiclava virens of rice false smut. Ustiloxins A and B as two main mycotoxins were determined conveniently by LC-ESI-MS in the water extract from rice false smut balls which were mostly composed of the chlamydospores and mycelia of the pathogen. Both ustiloxins A and B in the water extract were also quantitatively analyzed by HPLC. This is the first report on the determination and analysis of ustiloxins A and B simultaneously by LC-ESI-MS and HPLC in false smut balls of rice.

Show MeSH