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Determination and analysis of ustiloxins A and B by LC-ESI-MS and HPLC in false smut balls of rice.

Shan T, Sun W, Liu H, Gao S, Lu S, Wang M, Sun W, Chen Z, Wang S, Zhou L - Int J Mol Sci (2012)

Bottom Line: Ustiloxins are cyclopeptide mycotoxins produced by the pathogenic fungus Villosiclava virens of rice false smut.Ustiloxins A and B as two main mycotoxins were determined conveniently by LC-ESI-MS in the water extract from rice false smut balls which were mostly composed of the chlamydospores and mycelia of the pathogen.This is the first report on the determination and analysis of ustiloxins A and B simultaneously by LC-ESI-MS and HPLC in false smut balls of rice.

View Article: PubMed Central - PubMed

Affiliation: College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China; E-Mails: shan5400388@163.com (T.S.); sunweibo.1001@163.com (W.S.); hardyliuhao@163.com (H.L.); gaoshankonglong@163.com (S.G.); shiqionglu@126.com (S.L.); wangma@cau.edu.cn (M.W.); wxs@cau.edu.cn (W.S.).

ABSTRACT
Ustiloxins are cyclopeptide mycotoxins produced by the pathogenic fungus Villosiclava virens of rice false smut. Ustiloxins A and B as two main mycotoxins were determined conveniently by LC-ESI-MS in the water extract from rice false smut balls which were mostly composed of the chlamydospores and mycelia of the pathogen. Both ustiloxins A and B in the water extract were also quantitatively analyzed by HPLC. This is the first report on the determination and analysis of ustiloxins A and B simultaneously by LC-ESI-MS and HPLC in false smut balls of rice.

Show MeSH
Determination of the ustiloxins A and B by LC-ESI-MS. (A) and (B) show the spectra of HPLC-UV and total ion chromatograms, respectively. (C) and (D) show the ESI-MS spectra of ustiloxins B (peak 1) and A (peak 2), respectively, appearing in Figures 1A,B.
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f3-ijms-13-11275: Determination of the ustiloxins A and B by LC-ESI-MS. (A) and (B) show the spectra of HPLC-UV and total ion chromatograms, respectively. (C) and (D) show the ESI-MS spectra of ustiloxins B (peak 1) and A (peak 2), respectively, appearing in Figures 1A,B.

Mentions: With the same conditions as used in Figure 1, both the purified ustiloxins A and B were mixed for LC-ESI-MS analysis. Figure 3 shows the determination of ustiloxins A and B by LC-ESI-MS. At 646.3 m/z (Figure 3C) was the peak of the [M + H]+ ion of ustiloxin B that corresponds to peak 1, and at 674.3 m/z (Figure 3D) was the peak of the [M + H]+ ion of ustiloxin A that corresponds to peak 2 in Figure 3A,B. The retention times of ustiloxin B (peak 1) and ustiloxin A (peak 2) in Figure 3A,B were 8.3 min and 15.3 min, respectively, which were almost the same as peaks 1 and 3 in Figures 1A,B. The results confirmed that ustiloxins A and B as the two main ustiloxins in rice false smut balls can be determined by an LC-ESI-MS method.


Determination and analysis of ustiloxins A and B by LC-ESI-MS and HPLC in false smut balls of rice.

Shan T, Sun W, Liu H, Gao S, Lu S, Wang M, Sun W, Chen Z, Wang S, Zhou L - Int J Mol Sci (2012)

Determination of the ustiloxins A and B by LC-ESI-MS. (A) and (B) show the spectra of HPLC-UV and total ion chromatograms, respectively. (C) and (D) show the ESI-MS spectra of ustiloxins B (peak 1) and A (peak 2), respectively, appearing in Figures 1A,B.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3472744&req=5

f3-ijms-13-11275: Determination of the ustiloxins A and B by LC-ESI-MS. (A) and (B) show the spectra of HPLC-UV and total ion chromatograms, respectively. (C) and (D) show the ESI-MS spectra of ustiloxins B (peak 1) and A (peak 2), respectively, appearing in Figures 1A,B.
Mentions: With the same conditions as used in Figure 1, both the purified ustiloxins A and B were mixed for LC-ESI-MS analysis. Figure 3 shows the determination of ustiloxins A and B by LC-ESI-MS. At 646.3 m/z (Figure 3C) was the peak of the [M + H]+ ion of ustiloxin B that corresponds to peak 1, and at 674.3 m/z (Figure 3D) was the peak of the [M + H]+ ion of ustiloxin A that corresponds to peak 2 in Figure 3A,B. The retention times of ustiloxin B (peak 1) and ustiloxin A (peak 2) in Figure 3A,B were 8.3 min and 15.3 min, respectively, which were almost the same as peaks 1 and 3 in Figures 1A,B. The results confirmed that ustiloxins A and B as the two main ustiloxins in rice false smut balls can be determined by an LC-ESI-MS method.

Bottom Line: Ustiloxins are cyclopeptide mycotoxins produced by the pathogenic fungus Villosiclava virens of rice false smut.Ustiloxins A and B as two main mycotoxins were determined conveniently by LC-ESI-MS in the water extract from rice false smut balls which were mostly composed of the chlamydospores and mycelia of the pathogen.This is the first report on the determination and analysis of ustiloxins A and B simultaneously by LC-ESI-MS and HPLC in false smut balls of rice.

View Article: PubMed Central - PubMed

Affiliation: College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China; E-Mails: shan5400388@163.com (T.S.); sunweibo.1001@163.com (W.S.); hardyliuhao@163.com (H.L.); gaoshankonglong@163.com (S.G.); shiqionglu@126.com (S.L.); wangma@cau.edu.cn (M.W.); wxs@cau.edu.cn (W.S.).

ABSTRACT
Ustiloxins are cyclopeptide mycotoxins produced by the pathogenic fungus Villosiclava virens of rice false smut. Ustiloxins A and B as two main mycotoxins were determined conveniently by LC-ESI-MS in the water extract from rice false smut balls which were mostly composed of the chlamydospores and mycelia of the pathogen. Both ustiloxins A and B in the water extract were also quantitatively analyzed by HPLC. This is the first report on the determination and analysis of ustiloxins A and B simultaneously by LC-ESI-MS and HPLC in false smut balls of rice.

Show MeSH