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Determination and analysis of ustiloxins A and B by LC-ESI-MS and HPLC in false smut balls of rice.

Shan T, Sun W, Liu H, Gao S, Lu S, Wang M, Sun W, Chen Z, Wang S, Zhou L - Int J Mol Sci (2012)

Bottom Line: Ustiloxins are cyclopeptide mycotoxins produced by the pathogenic fungus Villosiclava virens of rice false smut.Ustiloxins A and B as two main mycotoxins were determined conveniently by LC-ESI-MS in the water extract from rice false smut balls which were mostly composed of the chlamydospores and mycelia of the pathogen.This is the first report on the determination and analysis of ustiloxins A and B simultaneously by LC-ESI-MS and HPLC in false smut balls of rice.

View Article: PubMed Central - PubMed

Affiliation: College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China; E-Mails: shan5400388@163.com (T.S.); sunweibo.1001@163.com (W.S.); hardyliuhao@163.com (H.L.); gaoshankonglong@163.com (S.G.); shiqionglu@126.com (S.L.); wangma@cau.edu.cn (M.W.); wxs@cau.edu.cn (W.S.).

ABSTRACT
Ustiloxins are cyclopeptide mycotoxins produced by the pathogenic fungus Villosiclava virens of rice false smut. Ustiloxins A and B as two main mycotoxins were determined conveniently by LC-ESI-MS in the water extract from rice false smut balls which were mostly composed of the chlamydospores and mycelia of the pathogen. Both ustiloxins A and B in the water extract were also quantitatively analyzed by HPLC. This is the first report on the determination and analysis of ustiloxins A and B simultaneously by LC-ESI-MS and HPLC in false smut balls of rice.

Show MeSH
Chemical structures of the ustiloxins A (1) and B (2).
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Related In: Results  -  Collection

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f2-ijms-13-11275: Chemical structures of the ustiloxins A (1) and B (2).

Mentions: After repeated column chromatographic purification on macroporous adsorption resin HP-20, dialysis, GH gel ODS-AQ, Sephadex LH-20, and Sephadex Q15, the water extract of rice false smut balls afforded two compounds (1 and 2). After comparing their physicochemical and spectrometric data with those reported in the literature, they were identified as ustiloxins A (1) [10], and B (2) [1,11], respectively, whose structures are shown in Figure 2.


Determination and analysis of ustiloxins A and B by LC-ESI-MS and HPLC in false smut balls of rice.

Shan T, Sun W, Liu H, Gao S, Lu S, Wang M, Sun W, Chen Z, Wang S, Zhou L - Int J Mol Sci (2012)

Chemical structures of the ustiloxins A (1) and B (2).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3472744&req=5

f2-ijms-13-11275: Chemical structures of the ustiloxins A (1) and B (2).
Mentions: After repeated column chromatographic purification on macroporous adsorption resin HP-20, dialysis, GH gel ODS-AQ, Sephadex LH-20, and Sephadex Q15, the water extract of rice false smut balls afforded two compounds (1 and 2). After comparing their physicochemical and spectrometric data with those reported in the literature, they were identified as ustiloxins A (1) [10], and B (2) [1,11], respectively, whose structures are shown in Figure 2.

Bottom Line: Ustiloxins are cyclopeptide mycotoxins produced by the pathogenic fungus Villosiclava virens of rice false smut.Ustiloxins A and B as two main mycotoxins were determined conveniently by LC-ESI-MS in the water extract from rice false smut balls which were mostly composed of the chlamydospores and mycelia of the pathogen.This is the first report on the determination and analysis of ustiloxins A and B simultaneously by LC-ESI-MS and HPLC in false smut balls of rice.

View Article: PubMed Central - PubMed

Affiliation: College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China; E-Mails: shan5400388@163.com (T.S.); sunweibo.1001@163.com (W.S.); hardyliuhao@163.com (H.L.); gaoshankonglong@163.com (S.G.); shiqionglu@126.com (S.L.); wangma@cau.edu.cn (M.W.); wxs@cau.edu.cn (W.S.).

ABSTRACT
Ustiloxins are cyclopeptide mycotoxins produced by the pathogenic fungus Villosiclava virens of rice false smut. Ustiloxins A and B as two main mycotoxins were determined conveniently by LC-ESI-MS in the water extract from rice false smut balls which were mostly composed of the chlamydospores and mycelia of the pathogen. Both ustiloxins A and B in the water extract were also quantitatively analyzed by HPLC. This is the first report on the determination and analysis of ustiloxins A and B simultaneously by LC-ESI-MS and HPLC in false smut balls of rice.

Show MeSH