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Quantification and purification of mangiferin from Chinese Mango (Mangifera indica L.) cultivars and its protective effect on human umbilical vein endothelial cells under H(2)O(2)-induced stress.

Luo F, Lv Q, Zhao Y, Hu G, Huang G, Zhang J, Sun C, Li X, Chen K - Int J Mol Sci (2012)

Bottom Line: In addition, it showed significant protective effects on human umbilical vein endothelial cells (HUVEC) under H(2)O(2)-induced stress.Cells treated with mangiferin resulted in significant enhanced cell survival under of H(2)O(2) stress.Therefore, mangiferin from mango fruit provides a promising perspective for the prevention of oxidative stress-associated diseases.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China; E-Mails: fenglei2012@zju.edu.cn (F.L.); lvqiang_85126@126.com (Q.L.); zhjk2010@zju.edu.cn (J.Z.); adesun2006@zju.edu.cn (C.S.); akun@zju.edu.cn (K.C.).

ABSTRACT
Mangiferin is a natural xanthonoid with various biological activities. Quantification of mangiferin in fruit peel, pulp, and seed kernel was carried out in 11 Chinese mango (Mangifera indica L.) cultivars. The highest mangiferin content was found in the peel of Lvpimang (LPM) fruit (7.49 mg/g DW). Efficient purification of mangiferin from mango fruit peel was then established for the first time by combination of macroporous HPD100 resin chromatography with optimized high-speed counter-current chromatography (HSCCC). Purified mangiferin was identified by both HPLC and LC-MS, and it showed higher DPPH(•) free-radical scavenging capacities and ferric reducing ability of plasma (FRAP) than by l-ascorbic acid (Vc) or Trolox. In addition, it showed significant protective effects on human umbilical vein endothelial cells (HUVEC) under H(2)O(2)-induced stress. Cells treated with mangiferin resulted in significant enhanced cell survival under of H(2)O(2) stress. Therefore, mangiferin from mango fruit provides a promising perspective for the prevention of oxidative stress-associated diseases.

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Related in: MedlinePlus

Protective effect of purified mangiferin on human umbilical vein endothelial cells (HUVEC) under H2O2-induced stress for 24 h. The rates of cell survival are shown as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, compared to H2O2 treatment only (as each control) respectively.
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f6-ijms-13-11260: Protective effect of purified mangiferin on human umbilical vein endothelial cells (HUVEC) under H2O2-induced stress for 24 h. The rates of cell survival are shown as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, compared to H2O2 treatment only (as each control) respectively.

Mentions: Intracellular balance of oxidant and antioxidant mechanisms determines endothelial function. Increasing oxidative stress can cause significant cell toxicity or even apoptosis, impair endothelial functions, and may increase the risk of chronic vascular disease such as atherosclerosis, cardiovascular injury, diabetic angiopathies, etc. [21,22]. In the present study, by using the model system HUVEC, the survival rates of cells exposed to varying concentrations of H2O2 with or without exogenous antioxidant mangiferin were evaluated. Results showed that H2O2 treatment of HUVEC caused significant decrease of cell survival. Compared with the control (0 mmol/L of H2O2), the cell survival rates were only 82.45 ± 5.47%, 48.87 ± 7.27%, and 14.14 ± 0.25% after 0.0625, 0.125, or 0.25 mmol/L of H2O2 treatment, respectively (Figure 6). Mangiferin at concentration of 10 μg/mL showed significant protective effect on HUVEC with the survival rates of 105.29 ± 0.93% under 0.0625 mmol/L of H2O2 (p < 0.01), 82.70 ± 1.08% under 0.125 mmol/L of H2O2 treatment (p < 0.01), 17.00 ± 1.08% under 0.25 mmol/L of H2O2 treatment (p < 0.05), when compared with their own controls (H2O2 treatment only), respectively. At a concentration of 20 μg/mL, mangiferin treatment resulted in the survival rates of 118.89 ± 5.49%, 83.83 ± 3.30%, and 19.23 ± 1.67% under 0.0625, 0.125, or 0.25 mmol/L of H2O2 treatment, respectively, which were all significant higher than their own controls (p < 0.01) (Figure 6).


Quantification and purification of mangiferin from Chinese Mango (Mangifera indica L.) cultivars and its protective effect on human umbilical vein endothelial cells under H(2)O(2)-induced stress.

Luo F, Lv Q, Zhao Y, Hu G, Huang G, Zhang J, Sun C, Li X, Chen K - Int J Mol Sci (2012)

Protective effect of purified mangiferin on human umbilical vein endothelial cells (HUVEC) under H2O2-induced stress for 24 h. The rates of cell survival are shown as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, compared to H2O2 treatment only (as each control) respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3472743&req=5

f6-ijms-13-11260: Protective effect of purified mangiferin on human umbilical vein endothelial cells (HUVEC) under H2O2-induced stress for 24 h. The rates of cell survival are shown as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, compared to H2O2 treatment only (as each control) respectively.
Mentions: Intracellular balance of oxidant and antioxidant mechanisms determines endothelial function. Increasing oxidative stress can cause significant cell toxicity or even apoptosis, impair endothelial functions, and may increase the risk of chronic vascular disease such as atherosclerosis, cardiovascular injury, diabetic angiopathies, etc. [21,22]. In the present study, by using the model system HUVEC, the survival rates of cells exposed to varying concentrations of H2O2 with or without exogenous antioxidant mangiferin were evaluated. Results showed that H2O2 treatment of HUVEC caused significant decrease of cell survival. Compared with the control (0 mmol/L of H2O2), the cell survival rates were only 82.45 ± 5.47%, 48.87 ± 7.27%, and 14.14 ± 0.25% after 0.0625, 0.125, or 0.25 mmol/L of H2O2 treatment, respectively (Figure 6). Mangiferin at concentration of 10 μg/mL showed significant protective effect on HUVEC with the survival rates of 105.29 ± 0.93% under 0.0625 mmol/L of H2O2 (p < 0.01), 82.70 ± 1.08% under 0.125 mmol/L of H2O2 treatment (p < 0.01), 17.00 ± 1.08% under 0.25 mmol/L of H2O2 treatment (p < 0.05), when compared with their own controls (H2O2 treatment only), respectively. At a concentration of 20 μg/mL, mangiferin treatment resulted in the survival rates of 118.89 ± 5.49%, 83.83 ± 3.30%, and 19.23 ± 1.67% under 0.0625, 0.125, or 0.25 mmol/L of H2O2 treatment, respectively, which were all significant higher than their own controls (p < 0.01) (Figure 6).

Bottom Line: In addition, it showed significant protective effects on human umbilical vein endothelial cells (HUVEC) under H(2)O(2)-induced stress.Cells treated with mangiferin resulted in significant enhanced cell survival under of H(2)O(2) stress.Therefore, mangiferin from mango fruit provides a promising perspective for the prevention of oxidative stress-associated diseases.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China; E-Mails: fenglei2012@zju.edu.cn (F.L.); lvqiang_85126@126.com (Q.L.); zhjk2010@zju.edu.cn (J.Z.); adesun2006@zju.edu.cn (C.S.); akun@zju.edu.cn (K.C.).

ABSTRACT
Mangiferin is a natural xanthonoid with various biological activities. Quantification of mangiferin in fruit peel, pulp, and seed kernel was carried out in 11 Chinese mango (Mangifera indica L.) cultivars. The highest mangiferin content was found in the peel of Lvpimang (LPM) fruit (7.49 mg/g DW). Efficient purification of mangiferin from mango fruit peel was then established for the first time by combination of macroporous HPD100 resin chromatography with optimized high-speed counter-current chromatography (HSCCC). Purified mangiferin was identified by both HPLC and LC-MS, and it showed higher DPPH(•) free-radical scavenging capacities and ferric reducing ability of plasma (FRAP) than by l-ascorbic acid (Vc) or Trolox. In addition, it showed significant protective effects on human umbilical vein endothelial cells (HUVEC) under H(2)O(2)-induced stress. Cells treated with mangiferin resulted in significant enhanced cell survival under of H(2)O(2) stress. Therefore, mangiferin from mango fruit provides a promising perspective for the prevention of oxidative stress-associated diseases.

Show MeSH
Related in: MedlinePlus