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Quantification and purification of mangiferin from Chinese Mango (Mangifera indica L.) cultivars and its protective effect on human umbilical vein endothelial cells under H(2)O(2)-induced stress.

Luo F, Lv Q, Zhao Y, Hu G, Huang G, Zhang J, Sun C, Li X, Chen K - Int J Mol Sci (2012)

Bottom Line: In addition, it showed significant protective effects on human umbilical vein endothelial cells (HUVEC) under H(2)O(2)-induced stress.Cells treated with mangiferin resulted in significant enhanced cell survival under of H(2)O(2) stress.Therefore, mangiferin from mango fruit provides a promising perspective for the prevention of oxidative stress-associated diseases.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China; E-Mails: fenglei2012@zju.edu.cn (F.L.); lvqiang_85126@126.com (Q.L.); zhjk2010@zju.edu.cn (J.Z.); adesun2006@zju.edu.cn (C.S.); akun@zju.edu.cn (K.C.).

ABSTRACT
Mangiferin is a natural xanthonoid with various biological activities. Quantification of mangiferin in fruit peel, pulp, and seed kernel was carried out in 11 Chinese mango (Mangifera indica L.) cultivars. The highest mangiferin content was found in the peel of Lvpimang (LPM) fruit (7.49 mg/g DW). Efficient purification of mangiferin from mango fruit peel was then established for the first time by combination of macroporous HPD100 resin chromatography with optimized high-speed counter-current chromatography (HSCCC). Purified mangiferin was identified by both HPLC and LC-MS, and it showed higher DPPH(•) free-radical scavenging capacities and ferric reducing ability of plasma (FRAP) than by l-ascorbic acid (Vc) or Trolox. In addition, it showed significant protective effects on human umbilical vein endothelial cells (HUVEC) under H(2)O(2)-induced stress. Cells treated with mangiferin resulted in significant enhanced cell survival under of H(2)O(2) stress. Therefore, mangiferin from mango fruit provides a promising perspective for the prevention of oxidative stress-associated diseases.

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Related in: MedlinePlus

Antioxidant activity of mangiferin assayed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) (A) and ferric reducing ability of plasma (FRAP) (B).
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f5-ijms-13-11260: Antioxidant activity of mangiferin assayed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) (A) and ferric reducing ability of plasma (FRAP) (B).

Mentions: Oxidative stress plays an important role in the pathogenesis of many chronic diseases such as atherosclerosis and cardiovascular disease and it is well established that dietary polyphenolics compounds play significant roles in the prevention of these stress-associated diseases [20]. In the present study, the purified mangiferin was analyzed for their DPPH• scavenging activity and reducing power by FRAP. At all the concentrations tested, mangiferin showed higher radical scavenging capacity than that of l-ascorbic acid (Vc) or Trolox, and it showed dose-responsive effect in both antioxidant assays. It showed 68.03 ± 0.21% DPPH• scavenging rate at the concentration of 10 μmol/L (Figure 5A). In FRAP assay, the reducing power enhanced with the increased concentrations of mangiferin and it was significantly higher than that of Vc or Trolox at the concentration of 50 μmol/L (Figure 5B).


Quantification and purification of mangiferin from Chinese Mango (Mangifera indica L.) cultivars and its protective effect on human umbilical vein endothelial cells under H(2)O(2)-induced stress.

Luo F, Lv Q, Zhao Y, Hu G, Huang G, Zhang J, Sun C, Li X, Chen K - Int J Mol Sci (2012)

Antioxidant activity of mangiferin assayed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) (A) and ferric reducing ability of plasma (FRAP) (B).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3472743&req=5

f5-ijms-13-11260: Antioxidant activity of mangiferin assayed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) (A) and ferric reducing ability of plasma (FRAP) (B).
Mentions: Oxidative stress plays an important role in the pathogenesis of many chronic diseases such as atherosclerosis and cardiovascular disease and it is well established that dietary polyphenolics compounds play significant roles in the prevention of these stress-associated diseases [20]. In the present study, the purified mangiferin was analyzed for their DPPH• scavenging activity and reducing power by FRAP. At all the concentrations tested, mangiferin showed higher radical scavenging capacity than that of l-ascorbic acid (Vc) or Trolox, and it showed dose-responsive effect in both antioxidant assays. It showed 68.03 ± 0.21% DPPH• scavenging rate at the concentration of 10 μmol/L (Figure 5A). In FRAP assay, the reducing power enhanced with the increased concentrations of mangiferin and it was significantly higher than that of Vc or Trolox at the concentration of 50 μmol/L (Figure 5B).

Bottom Line: In addition, it showed significant protective effects on human umbilical vein endothelial cells (HUVEC) under H(2)O(2)-induced stress.Cells treated with mangiferin resulted in significant enhanced cell survival under of H(2)O(2) stress.Therefore, mangiferin from mango fruit provides a promising perspective for the prevention of oxidative stress-associated diseases.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China; E-Mails: fenglei2012@zju.edu.cn (F.L.); lvqiang_85126@126.com (Q.L.); zhjk2010@zju.edu.cn (J.Z.); adesun2006@zju.edu.cn (C.S.); akun@zju.edu.cn (K.C.).

ABSTRACT
Mangiferin is a natural xanthonoid with various biological activities. Quantification of mangiferin in fruit peel, pulp, and seed kernel was carried out in 11 Chinese mango (Mangifera indica L.) cultivars. The highest mangiferin content was found in the peel of Lvpimang (LPM) fruit (7.49 mg/g DW). Efficient purification of mangiferin from mango fruit peel was then established for the first time by combination of macroporous HPD100 resin chromatography with optimized high-speed counter-current chromatography (HSCCC). Purified mangiferin was identified by both HPLC and LC-MS, and it showed higher DPPH(•) free-radical scavenging capacities and ferric reducing ability of plasma (FRAP) than by l-ascorbic acid (Vc) or Trolox. In addition, it showed significant protective effects on human umbilical vein endothelial cells (HUVEC) under H(2)O(2)-induced stress. Cells treated with mangiferin resulted in significant enhanced cell survival under of H(2)O(2) stress. Therefore, mangiferin from mango fruit provides a promising perspective for the prevention of oxidative stress-associated diseases.

Show MeSH
Related in: MedlinePlus