Limits...
Quantification and purification of mangiferin from Chinese Mango (Mangifera indica L.) cultivars and its protective effect on human umbilical vein endothelial cells under H(2)O(2)-induced stress.

Luo F, Lv Q, Zhao Y, Hu G, Huang G, Zhang J, Sun C, Li X, Chen K - Int J Mol Sci (2012)

Bottom Line: In addition, it showed significant protective effects on human umbilical vein endothelial cells (HUVEC) under H(2)O(2)-induced stress.Cells treated with mangiferin resulted in significant enhanced cell survival under of H(2)O(2) stress.Therefore, mangiferin from mango fruit provides a promising perspective for the prevention of oxidative stress-associated diseases.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China; E-Mails: fenglei2012@zju.edu.cn (F.L.); lvqiang_85126@126.com (Q.L.); zhjk2010@zju.edu.cn (J.Z.); adesun2006@zju.edu.cn (C.S.); akun@zju.edu.cn (K.C.).

ABSTRACT
Mangiferin is a natural xanthonoid with various biological activities. Quantification of mangiferin in fruit peel, pulp, and seed kernel was carried out in 11 Chinese mango (Mangifera indica L.) cultivars. The highest mangiferin content was found in the peel of Lvpimang (LPM) fruit (7.49 mg/g DW). Efficient purification of mangiferin from mango fruit peel was then established for the first time by combination of macroporous HPD100 resin chromatography with optimized high-speed counter-current chromatography (HSCCC). Purified mangiferin was identified by both HPLC and LC-MS, and it showed higher DPPH(•) free-radical scavenging capacities and ferric reducing ability of plasma (FRAP) than by l-ascorbic acid (Vc) or Trolox. In addition, it showed significant protective effects on human umbilical vein endothelial cells (HUVEC) under H(2)O(2)-induced stress. Cells treated with mangiferin resulted in significant enhanced cell survival under of H(2)O(2) stress. Therefore, mangiferin from mango fruit provides a promising perspective for the prevention of oxidative stress-associated diseases.

Show MeSH

Related in: MedlinePlus

High performance liquid chromatography (HPLC) chromatogram of crude extract before (A) and after (B) treatment with HPD100 resin, the HSCCC purified product (C), and LC-MS chromatogram of the final purified mangiferin (D) (λ= 258 nm).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC3472743&req=5

f4-ijms-13-11260: High performance liquid chromatography (HPLC) chromatogram of crude extract before (A) and after (B) treatment with HPD100 resin, the HSCCC purified product (C), and LC-MS chromatogram of the final purified mangiferin (D) (λ= 258 nm).

Mentions: The effect of the two-step purification was shown in Figure 4, and the purities and recoveries of mangiferin in different procedures were summarized in Table 3. The purity of mangiferin in the crude extract of LPM peel was as low as 0.75%. According to the HPLC chromatograms, the majority of UV absorbing impurities were removed by HPD 100 resin chromatography (Figure 4A,B). After one-step HPD100 resin purification, the purity increased to 37.80%, which was 50.4-fold that of the crude extract. The mangiferin recovery was 85.15% (Table 3). In addition, HSCCC purification resulted in 74.3 mg mangiferin with 99.13% purity (Figure 4C) and the recovery rate was 77.94% from a 250 mg sample (Table 3). Further identification of the purified mangiferin was confirmed by HPLC-ESI-MS (Figure 4D), where the [M-H]− ion at m/z 421.0 indicating the molecular weight of mangiferin to be 422. Additionally, three C-glycoside xanthone typical product ions at m/z 403.0 [M-H2O]−, 331.0 [M-C3H6O3]−, 301.0 [M-C4H8O4]− were detected in LC-MS2 chromatogram, the fragmentation pattern of which is consistent with the proposed mechanistic pathway by Ma et al. [19]. Based on these data, the structure of the purified compound could be identified as mangiferin (C19H18O11). Therefore, both the HPLC and LC-MS data confirmed the purified compound to be mangiferin. These results demonstrated that HPD100 resin chromatography combined with optimized HSCCC resulted in efficient purification of mangiferin from LPM peel.


Quantification and purification of mangiferin from Chinese Mango (Mangifera indica L.) cultivars and its protective effect on human umbilical vein endothelial cells under H(2)O(2)-induced stress.

Luo F, Lv Q, Zhao Y, Hu G, Huang G, Zhang J, Sun C, Li X, Chen K - Int J Mol Sci (2012)

High performance liquid chromatography (HPLC) chromatogram of crude extract before (A) and after (B) treatment with HPD100 resin, the HSCCC purified product (C), and LC-MS chromatogram of the final purified mangiferin (D) (λ= 258 nm).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3472743&req=5

f4-ijms-13-11260: High performance liquid chromatography (HPLC) chromatogram of crude extract before (A) and after (B) treatment with HPD100 resin, the HSCCC purified product (C), and LC-MS chromatogram of the final purified mangiferin (D) (λ= 258 nm).
Mentions: The effect of the two-step purification was shown in Figure 4, and the purities and recoveries of mangiferin in different procedures were summarized in Table 3. The purity of mangiferin in the crude extract of LPM peel was as low as 0.75%. According to the HPLC chromatograms, the majority of UV absorbing impurities were removed by HPD 100 resin chromatography (Figure 4A,B). After one-step HPD100 resin purification, the purity increased to 37.80%, which was 50.4-fold that of the crude extract. The mangiferin recovery was 85.15% (Table 3). In addition, HSCCC purification resulted in 74.3 mg mangiferin with 99.13% purity (Figure 4C) and the recovery rate was 77.94% from a 250 mg sample (Table 3). Further identification of the purified mangiferin was confirmed by HPLC-ESI-MS (Figure 4D), where the [M-H]− ion at m/z 421.0 indicating the molecular weight of mangiferin to be 422. Additionally, three C-glycoside xanthone typical product ions at m/z 403.0 [M-H2O]−, 331.0 [M-C3H6O3]−, 301.0 [M-C4H8O4]− were detected in LC-MS2 chromatogram, the fragmentation pattern of which is consistent with the proposed mechanistic pathway by Ma et al. [19]. Based on these data, the structure of the purified compound could be identified as mangiferin (C19H18O11). Therefore, both the HPLC and LC-MS data confirmed the purified compound to be mangiferin. These results demonstrated that HPD100 resin chromatography combined with optimized HSCCC resulted in efficient purification of mangiferin from LPM peel.

Bottom Line: In addition, it showed significant protective effects on human umbilical vein endothelial cells (HUVEC) under H(2)O(2)-induced stress.Cells treated with mangiferin resulted in significant enhanced cell survival under of H(2)O(2) stress.Therefore, mangiferin from mango fruit provides a promising perspective for the prevention of oxidative stress-associated diseases.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China; E-Mails: fenglei2012@zju.edu.cn (F.L.); lvqiang_85126@126.com (Q.L.); zhjk2010@zju.edu.cn (J.Z.); adesun2006@zju.edu.cn (C.S.); akun@zju.edu.cn (K.C.).

ABSTRACT
Mangiferin is a natural xanthonoid with various biological activities. Quantification of mangiferin in fruit peel, pulp, and seed kernel was carried out in 11 Chinese mango (Mangifera indica L.) cultivars. The highest mangiferin content was found in the peel of Lvpimang (LPM) fruit (7.49 mg/g DW). Efficient purification of mangiferin from mango fruit peel was then established for the first time by combination of macroporous HPD100 resin chromatography with optimized high-speed counter-current chromatography (HSCCC). Purified mangiferin was identified by both HPLC and LC-MS, and it showed higher DPPH(•) free-radical scavenging capacities and ferric reducing ability of plasma (FRAP) than by l-ascorbic acid (Vc) or Trolox. In addition, it showed significant protective effects on human umbilical vein endothelial cells (HUVEC) under H(2)O(2)-induced stress. Cells treated with mangiferin resulted in significant enhanced cell survival under of H(2)O(2) stress. Therefore, mangiferin from mango fruit provides a promising perspective for the prevention of oxidative stress-associated diseases.

Show MeSH
Related in: MedlinePlus