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Quantification and purification of mangiferin from Chinese Mango (Mangifera indica L.) cultivars and its protective effect on human umbilical vein endothelial cells under H(2)O(2)-induced stress.

Luo F, Lv Q, Zhao Y, Hu G, Huang G, Zhang J, Sun C, Li X, Chen K - Int J Mol Sci (2012)

Bottom Line: In addition, it showed significant protective effects on human umbilical vein endothelial cells (HUVEC) under H(2)O(2)-induced stress.Cells treated with mangiferin resulted in significant enhanced cell survival under of H(2)O(2) stress.Therefore, mangiferin from mango fruit provides a promising perspective for the prevention of oxidative stress-associated diseases.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China; E-Mails: fenglei2012@zju.edu.cn (F.L.); lvqiang_85126@126.com (Q.L.); zhjk2010@zju.edu.cn (J.Z.); adesun2006@zju.edu.cn (C.S.); akun@zju.edu.cn (K.C.).

ABSTRACT
Mangiferin is a natural xanthonoid with various biological activities. Quantification of mangiferin in fruit peel, pulp, and seed kernel was carried out in 11 Chinese mango (Mangifera indica L.) cultivars. The highest mangiferin content was found in the peel of Lvpimang (LPM) fruit (7.49 mg/g DW). Efficient purification of mangiferin from mango fruit peel was then established for the first time by combination of macroporous HPD100 resin chromatography with optimized high-speed counter-current chromatography (HSCCC). Purified mangiferin was identified by both HPLC and LC-MS, and it showed higher DPPH(•) free-radical scavenging capacities and ferric reducing ability of plasma (FRAP) than by l-ascorbic acid (Vc) or Trolox. In addition, it showed significant protective effects on human umbilical vein endothelial cells (HUVEC) under H(2)O(2)-induced stress. Cells treated with mangiferin resulted in significant enhanced cell survival under of H(2)O(2) stress. Therefore, mangiferin from mango fruit provides a promising perspective for the prevention of oxidative stress-associated diseases.

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Related in: MedlinePlus

High-speed counter-current chromatography (HSCCC) chromatogram of separation of mangiferin from resin-refined sample (A: Single injection mode; B: Continuous injection mode). Two-phase solvent system: ethyl acetate–n-butanol–water (4:1:5, v/v/v); stationary phase: upper phase; mobile phase: lower phase; flow rate: 2.0 mL/min; revolution speed: 800 rpm; detection wavelength: 258 nm.
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f3-ijms-13-11260: High-speed counter-current chromatography (HSCCC) chromatogram of separation of mangiferin from resin-refined sample (A: Single injection mode; B: Continuous injection mode). Two-phase solvent system: ethyl acetate–n-butanol–water (4:1:5, v/v/v); stationary phase: upper phase; mobile phase: lower phase; flow rate: 2.0 mL/min; revolution speed: 800 rpm; detection wavelength: 258 nm.

Mentions: For single injection mode, effluents in tubes from #6 to #46 were proved by HPLC analysis to contain pure mangiferin and they were hence combined (Figure 3A). For continuous injection mode, effluents in tube #10-50, tube #77-117, tube #142-182, and tube #209-249 were proved to contain pure mangiferin and they were hence combined (Figure 3B).


Quantification and purification of mangiferin from Chinese Mango (Mangifera indica L.) cultivars and its protective effect on human umbilical vein endothelial cells under H(2)O(2)-induced stress.

Luo F, Lv Q, Zhao Y, Hu G, Huang G, Zhang J, Sun C, Li X, Chen K - Int J Mol Sci (2012)

High-speed counter-current chromatography (HSCCC) chromatogram of separation of mangiferin from resin-refined sample (A: Single injection mode; B: Continuous injection mode). Two-phase solvent system: ethyl acetate–n-butanol–water (4:1:5, v/v/v); stationary phase: upper phase; mobile phase: lower phase; flow rate: 2.0 mL/min; revolution speed: 800 rpm; detection wavelength: 258 nm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3472743&req=5

f3-ijms-13-11260: High-speed counter-current chromatography (HSCCC) chromatogram of separation of mangiferin from resin-refined sample (A: Single injection mode; B: Continuous injection mode). Two-phase solvent system: ethyl acetate–n-butanol–water (4:1:5, v/v/v); stationary phase: upper phase; mobile phase: lower phase; flow rate: 2.0 mL/min; revolution speed: 800 rpm; detection wavelength: 258 nm.
Mentions: For single injection mode, effluents in tubes from #6 to #46 were proved by HPLC analysis to contain pure mangiferin and they were hence combined (Figure 3A). For continuous injection mode, effluents in tube #10-50, tube #77-117, tube #142-182, and tube #209-249 were proved to contain pure mangiferin and they were hence combined (Figure 3B).

Bottom Line: In addition, it showed significant protective effects on human umbilical vein endothelial cells (HUVEC) under H(2)O(2)-induced stress.Cells treated with mangiferin resulted in significant enhanced cell survival under of H(2)O(2) stress.Therefore, mangiferin from mango fruit provides a promising perspective for the prevention of oxidative stress-associated diseases.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China; E-Mails: fenglei2012@zju.edu.cn (F.L.); lvqiang_85126@126.com (Q.L.); zhjk2010@zju.edu.cn (J.Z.); adesun2006@zju.edu.cn (C.S.); akun@zju.edu.cn (K.C.).

ABSTRACT
Mangiferin is a natural xanthonoid with various biological activities. Quantification of mangiferin in fruit peel, pulp, and seed kernel was carried out in 11 Chinese mango (Mangifera indica L.) cultivars. The highest mangiferin content was found in the peel of Lvpimang (LPM) fruit (7.49 mg/g DW). Efficient purification of mangiferin from mango fruit peel was then established for the first time by combination of macroporous HPD100 resin chromatography with optimized high-speed counter-current chromatography (HSCCC). Purified mangiferin was identified by both HPLC and LC-MS, and it showed higher DPPH(•) free-radical scavenging capacities and ferric reducing ability of plasma (FRAP) than by l-ascorbic acid (Vc) or Trolox. In addition, it showed significant protective effects on human umbilical vein endothelial cells (HUVEC) under H(2)O(2)-induced stress. Cells treated with mangiferin resulted in significant enhanced cell survival under of H(2)O(2) stress. Therefore, mangiferin from mango fruit provides a promising perspective for the prevention of oxidative stress-associated diseases.

Show MeSH
Related in: MedlinePlus