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Quantification and purification of mangiferin from Chinese Mango (Mangifera indica L.) cultivars and its protective effect on human umbilical vein endothelial cells under H(2)O(2)-induced stress.

Luo F, Lv Q, Zhao Y, Hu G, Huang G, Zhang J, Sun C, Li X, Chen K - Int J Mol Sci (2012)

Bottom Line: In addition, it showed significant protective effects on human umbilical vein endothelial cells (HUVEC) under H(2)O(2)-induced stress.Cells treated with mangiferin resulted in significant enhanced cell survival under of H(2)O(2) stress.Therefore, mangiferin from mango fruit provides a promising perspective for the prevention of oxidative stress-associated diseases.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China; E-Mails: fenglei2012@zju.edu.cn (F.L.); lvqiang_85126@126.com (Q.L.); zhjk2010@zju.edu.cn (J.Z.); adesun2006@zju.edu.cn (C.S.); akun@zju.edu.cn (K.C.).

ABSTRACT
Mangiferin is a natural xanthonoid with various biological activities. Quantification of mangiferin in fruit peel, pulp, and seed kernel was carried out in 11 Chinese mango (Mangifera indica L.) cultivars. The highest mangiferin content was found in the peel of Lvpimang (LPM) fruit (7.49 mg/g DW). Efficient purification of mangiferin from mango fruit peel was then established for the first time by combination of macroporous HPD100 resin chromatography with optimized high-speed counter-current chromatography (HSCCC). Purified mangiferin was identified by both HPLC and LC-MS, and it showed higher DPPH(•) free-radical scavenging capacities and ferric reducing ability of plasma (FRAP) than by l-ascorbic acid (Vc) or Trolox. In addition, it showed significant protective effects on human umbilical vein endothelial cells (HUVEC) under H(2)O(2)-induced stress. Cells treated with mangiferin resulted in significant enhanced cell survival under of H(2)O(2) stress. Therefore, mangiferin from mango fruit provides a promising perspective for the prevention of oxidative stress-associated diseases.

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Structure of mangiferin.
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f1-ijms-13-11260: Structure of mangiferin.

Mentions: Among the various polyphenolic compounds found in the mango [6], mangiferin (C-2-β-d-glucopyranosyl-1,3,6,7-tetrahydroxyxanthone, also named C-glucosyl xanthone) is a distinct one (Figure 1). Mangiferin is a heat-stable molecule and a natural pharmacologically active phytochemical that was found to have various bioactivities, such as anti-inflammation [7], anti-diabetic [8], immunomodulatory [9], anti-tumor [10,11], and antioxidant [12,13]. It can promote endothelial cell migration during the angiogenesis and may therefore have promising prevention and therapeutic potentials on vascular diseases [14]. It bears a catechol moiety, which is important for its diverse biological activity.


Quantification and purification of mangiferin from Chinese Mango (Mangifera indica L.) cultivars and its protective effect on human umbilical vein endothelial cells under H(2)O(2)-induced stress.

Luo F, Lv Q, Zhao Y, Hu G, Huang G, Zhang J, Sun C, Li X, Chen K - Int J Mol Sci (2012)

Structure of mangiferin.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3472743&req=5

f1-ijms-13-11260: Structure of mangiferin.
Mentions: Among the various polyphenolic compounds found in the mango [6], mangiferin (C-2-β-d-glucopyranosyl-1,3,6,7-tetrahydroxyxanthone, also named C-glucosyl xanthone) is a distinct one (Figure 1). Mangiferin is a heat-stable molecule and a natural pharmacologically active phytochemical that was found to have various bioactivities, such as anti-inflammation [7], anti-diabetic [8], immunomodulatory [9], anti-tumor [10,11], and antioxidant [12,13]. It can promote endothelial cell migration during the angiogenesis and may therefore have promising prevention and therapeutic potentials on vascular diseases [14]. It bears a catechol moiety, which is important for its diverse biological activity.

Bottom Line: In addition, it showed significant protective effects on human umbilical vein endothelial cells (HUVEC) under H(2)O(2)-induced stress.Cells treated with mangiferin resulted in significant enhanced cell survival under of H(2)O(2) stress.Therefore, mangiferin from mango fruit provides a promising perspective for the prevention of oxidative stress-associated diseases.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China; E-Mails: fenglei2012@zju.edu.cn (F.L.); lvqiang_85126@126.com (Q.L.); zhjk2010@zju.edu.cn (J.Z.); adesun2006@zju.edu.cn (C.S.); akun@zju.edu.cn (K.C.).

ABSTRACT
Mangiferin is a natural xanthonoid with various biological activities. Quantification of mangiferin in fruit peel, pulp, and seed kernel was carried out in 11 Chinese mango (Mangifera indica L.) cultivars. The highest mangiferin content was found in the peel of Lvpimang (LPM) fruit (7.49 mg/g DW). Efficient purification of mangiferin from mango fruit peel was then established for the first time by combination of macroporous HPD100 resin chromatography with optimized high-speed counter-current chromatography (HSCCC). Purified mangiferin was identified by both HPLC and LC-MS, and it showed higher DPPH(•) free-radical scavenging capacities and ferric reducing ability of plasma (FRAP) than by l-ascorbic acid (Vc) or Trolox. In addition, it showed significant protective effects on human umbilical vein endothelial cells (HUVEC) under H(2)O(2)-induced stress. Cells treated with mangiferin resulted in significant enhanced cell survival under of H(2)O(2) stress. Therefore, mangiferin from mango fruit provides a promising perspective for the prevention of oxidative stress-associated diseases.

Show MeSH
Related in: MedlinePlus