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Nucleotide excision repair in cellular chromatin: studies with yeast from nucleotide to gene to genome.

Waters R, Evans K, Bennett M, Yu S, Reed S - Int J Mol Sci (2012)

Bottom Line: Here we review our development of, and results with, high resolution studies on global genome nucleotide excision repair (GGNER) in Saccharomyces cerevisiae.We consider results employing primarily MFA2 as a model gene, but also those with URA3 located at subtelomeric sequences.In the latter case we also see a role for acetylation at histone H4.

View Article: PubMed Central - PubMed

Affiliation: Institute of Cancer and Genetics, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, UK; E-Mails: evansKE3@cardiff.ac.uk (K.E.); bennettMR1@cardiff.ac.uk (M.B.); yuS@cardiff.ac.uk (S.Y.); reedSH1@cardiff.ac.uk (S.R.).

ABSTRACT
Here we review our development of, and results with, high resolution studies on global genome nucleotide excision repair (GGNER) in Saccharomyces cerevisiae. We have focused on how GGNER relates to histone acetylation for its functioning and we have identified the histone acetyl tranferase Gcn5 and acetylation at lysines 9/14 of histone H3 as a major factor in enabling efficient repair. We consider results employing primarily MFA2 as a model gene, but also those with URA3 located at subtelomeric sequences. In the latter case we also see a role for acetylation at histone H4. We then go on to outline the development of a high resolution genome-wide approach that enables one to examine correlations between histone modifications and the nucleotide excision repair (NER) of UV-induced cyclobutane pyrimidine dimers throughout entire genomes. This is an approach that will enable rapid advances in understanding the complexities of how compacted chromatin in chromosomes is processed to access DNA damage and then returned to its pre-damaged status to maintain epigenetic codes.

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Related in: MedlinePlus

Relative plots pre- and post- UV of histone H3 acetylation at lysines 9/14 (left hand graph) and histone H4 (right hand graph) in the 2 nucleosomes of the MFA2 regulatory region [16]. Data are in unirradiated a-cells where MFA2 is transcriptionally active, and in α cells where it is transcriptionally repressed and where cells were either unirradiated or given 150 J/m2 and then incubated for various times after UV.
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f6-ijms-13-11141: Relative plots pre- and post- UV of histone H3 acetylation at lysines 9/14 (left hand graph) and histone H4 (right hand graph) in the 2 nucleosomes of the MFA2 regulatory region [16]. Data are in unirradiated a-cells where MFA2 is transcriptionally active, and in α cells where it is transcriptionally repressed and where cells were either unirradiated or given 150 J/m2 and then incubated for various times after UV.

Mentions: In α mating type cells possessing the HAT Gcn5 there was a UV-induced increase in histone H3 acetylation at lysines 9/14 for the two nucleosomes in the MFA2 regulatory region of the transcriptionally repressed gene; most of this was mediated by Gcn5 (Figure 6). We have not examined whether this acetylation extends to the nucleosomes in the coding sequence of the gene. At the nucleosomes within the MFA2 regulatory region there is little increase in acetylation at histone H4 [16].


Nucleotide excision repair in cellular chromatin: studies with yeast from nucleotide to gene to genome.

Waters R, Evans K, Bennett M, Yu S, Reed S - Int J Mol Sci (2012)

Relative plots pre- and post- UV of histone H3 acetylation at lysines 9/14 (left hand graph) and histone H4 (right hand graph) in the 2 nucleosomes of the MFA2 regulatory region [16]. Data are in unirradiated a-cells where MFA2 is transcriptionally active, and in α cells where it is transcriptionally repressed and where cells were either unirradiated or given 150 J/m2 and then incubated for various times after UV.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3472735&req=5

f6-ijms-13-11141: Relative plots pre- and post- UV of histone H3 acetylation at lysines 9/14 (left hand graph) and histone H4 (right hand graph) in the 2 nucleosomes of the MFA2 regulatory region [16]. Data are in unirradiated a-cells where MFA2 is transcriptionally active, and in α cells where it is transcriptionally repressed and where cells were either unirradiated or given 150 J/m2 and then incubated for various times after UV.
Mentions: In α mating type cells possessing the HAT Gcn5 there was a UV-induced increase in histone H3 acetylation at lysines 9/14 for the two nucleosomes in the MFA2 regulatory region of the transcriptionally repressed gene; most of this was mediated by Gcn5 (Figure 6). We have not examined whether this acetylation extends to the nucleosomes in the coding sequence of the gene. At the nucleosomes within the MFA2 regulatory region there is little increase in acetylation at histone H4 [16].

Bottom Line: Here we review our development of, and results with, high resolution studies on global genome nucleotide excision repair (GGNER) in Saccharomyces cerevisiae.We consider results employing primarily MFA2 as a model gene, but also those with URA3 located at subtelomeric sequences.In the latter case we also see a role for acetylation at histone H4.

View Article: PubMed Central - PubMed

Affiliation: Institute of Cancer and Genetics, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, UK; E-Mails: evansKE3@cardiff.ac.uk (K.E.); bennettMR1@cardiff.ac.uk (M.B.); yuS@cardiff.ac.uk (S.Y.); reedSH1@cardiff.ac.uk (S.R.).

ABSTRACT
Here we review our development of, and results with, high resolution studies on global genome nucleotide excision repair (GGNER) in Saccharomyces cerevisiae. We have focused on how GGNER relates to histone acetylation for its functioning and we have identified the histone acetyl tranferase Gcn5 and acetylation at lysines 9/14 of histone H3 as a major factor in enabling efficient repair. We consider results employing primarily MFA2 as a model gene, but also those with URA3 located at subtelomeric sequences. In the latter case we also see a role for acetylation at histone H4. We then go on to outline the development of a high resolution genome-wide approach that enables one to examine correlations between histone modifications and the nucleotide excision repair (NER) of UV-induced cyclobutane pyrimidine dimers throughout entire genomes. This is an approach that will enable rapid advances in understanding the complexities of how compacted chromatin in chromosomes is processed to access DNA damage and then returned to its pre-damaged status to maintain epigenetic codes.

Show MeSH
Related in: MedlinePlus