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Tissue microarray-based evaluation of Chromatin Assembly Factor-1 (CAF-1)/p60 as tumour prognostic marker.

Mascolo M, Ilardi G, Merolla F, Russo D, Vecchione ML, de Rosa G, Staibano S - Int J Mol Sci (2012)

Bottom Line: We also analysed, for the first time, 30 larynx and 30 skin squamous cell carcinomas.Notably, a high degree of agreement was found between the CAF-1 p60 assessment on TMAs and on routine tissue sections.Our findings confirm the prognostic role of CAF-1 p60 and indicate TMA as a really advantageous method for CAF-1 p60 immunohistochemical screening, allowing savings on both tissue quantity and operator-time.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomorphological and Functional Sciences, Pathology Section, University of Naples "Federico II", Naples 80138, Italy; E-Mails: mmascol@gmail.com (M.M.); gennaro.ilardi@unina.it (G.I.); francesco.merolla@unina.it (F.M.); danielarusso83@yahoo.it (D.R.); marysilvio@alice.it (M.L.V.); gaderosa@unina.it (G.R.).

ABSTRACT
In this study we aimed to confirm the emerging role of Chromatin Assembly Factor 1 (CAF-1 p60) as a new proliferation and prognostic marker for cancer and to test the usefulness of the tissue microarray technique (TMA) for CAF-1 p60 rapid screening in several human malignancies. CAF-1 is a histone chaperone, regulating chromatin dynamics during DNA replication and repair in eukaryotics. TMA is a powerful high-throughput methodology in the study of cancer, allowing simultaneous assessment of different biomarkers within large numbers of tissue specimens. We generated TMA taking 3 mm diameter-core biopsies from oral squamous cell carcinoma, prostate cancer, salivary gland tumours and skin melanoma specimens, which had been previously tested for CAF-1 p60 on routine tissue sections. We also analysed, for the first time, 30 larynx and 30 skin squamous cell carcinomas. CAF-1 p60 resulted over-expressed in both the tissue sections and the TMA specimens, with the highest levels of expression in tumours which were more aggressive and metastasizing. Notably, a high degree of agreement was found between the CAF-1 p60 assessment on TMAs and on routine tissue sections. Our findings confirm the prognostic role of CAF-1 p60 and indicate TMA as a really advantageous method for CAF-1 p60 immunohistochemical screening, allowing savings on both tissue quantity and operator-time.

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Related in: MedlinePlus

Kaplan-Meier plots showing disease free survival for OSCC, PC, SGT, SM, LSCC AND SSCC patients grouped by the level of expression of CAF-1 p60. Tumor samples were stratified in three categories (+, ++, and +++) based on intensity of CAF-1 p60 immunostaining. The comparison between survival curves and p value was determined by a two-sided log-rank test. (a) Oral squamous cell carcinoma (OSCC); (b) prostate cancer (PC); (c) salivary gland tumour (SGT); (d) skin melanoma (SM); (e) laryngeal squamous cell carcinoma (LSCC); (f) skin squamous cell carcinoma (SSCC).
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f4-ijms-13-11044: Kaplan-Meier plots showing disease free survival for OSCC, PC, SGT, SM, LSCC AND SSCC patients grouped by the level of expression of CAF-1 p60. Tumor samples were stratified in three categories (+, ++, and +++) based on intensity of CAF-1 p60 immunostaining. The comparison between survival curves and p value was determined by a two-sided log-rank test. (a) Oral squamous cell carcinoma (OSCC); (b) prostate cancer (PC); (c) salivary gland tumour (SGT); (d) skin melanoma (SM); (e) laryngeal squamous cell carcinoma (LSCC); (f) skin squamous cell carcinoma (SSCC).

Mentions: Irrespective of the tumour histogenesis, CAF-1 p60 showed high levels of expression in all evaluated tumours (OSCC, PC, SM, SGTs and LSCC and SSCC) with the highest values in cases showing adverse events during the follow up (relapse, lymph node and/or distant metastasis, death from disease). Correlation between CAF-1 p60 expression and adverse events at follow-up proved to be statistically significant, as shown by Kaplan-Meier curves in Figure 4.


Tissue microarray-based evaluation of Chromatin Assembly Factor-1 (CAF-1)/p60 as tumour prognostic marker.

Mascolo M, Ilardi G, Merolla F, Russo D, Vecchione ML, de Rosa G, Staibano S - Int J Mol Sci (2012)

Kaplan-Meier plots showing disease free survival for OSCC, PC, SGT, SM, LSCC AND SSCC patients grouped by the level of expression of CAF-1 p60. Tumor samples were stratified in three categories (+, ++, and +++) based on intensity of CAF-1 p60 immunostaining. The comparison between survival curves and p value was determined by a two-sided log-rank test. (a) Oral squamous cell carcinoma (OSCC); (b) prostate cancer (PC); (c) salivary gland tumour (SGT); (d) skin melanoma (SM); (e) laryngeal squamous cell carcinoma (LSCC); (f) skin squamous cell carcinoma (SSCC).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3472729&req=5

f4-ijms-13-11044: Kaplan-Meier plots showing disease free survival for OSCC, PC, SGT, SM, LSCC AND SSCC patients grouped by the level of expression of CAF-1 p60. Tumor samples were stratified in three categories (+, ++, and +++) based on intensity of CAF-1 p60 immunostaining. The comparison between survival curves and p value was determined by a two-sided log-rank test. (a) Oral squamous cell carcinoma (OSCC); (b) prostate cancer (PC); (c) salivary gland tumour (SGT); (d) skin melanoma (SM); (e) laryngeal squamous cell carcinoma (LSCC); (f) skin squamous cell carcinoma (SSCC).
Mentions: Irrespective of the tumour histogenesis, CAF-1 p60 showed high levels of expression in all evaluated tumours (OSCC, PC, SM, SGTs and LSCC and SSCC) with the highest values in cases showing adverse events during the follow up (relapse, lymph node and/or distant metastasis, death from disease). Correlation between CAF-1 p60 expression and adverse events at follow-up proved to be statistically significant, as shown by Kaplan-Meier curves in Figure 4.

Bottom Line: We also analysed, for the first time, 30 larynx and 30 skin squamous cell carcinomas.Notably, a high degree of agreement was found between the CAF-1 p60 assessment on TMAs and on routine tissue sections.Our findings confirm the prognostic role of CAF-1 p60 and indicate TMA as a really advantageous method for CAF-1 p60 immunohistochemical screening, allowing savings on both tissue quantity and operator-time.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomorphological and Functional Sciences, Pathology Section, University of Naples "Federico II", Naples 80138, Italy; E-Mails: mmascol@gmail.com (M.M.); gennaro.ilardi@unina.it (G.I.); francesco.merolla@unina.it (F.M.); danielarusso83@yahoo.it (D.R.); marysilvio@alice.it (M.L.V.); gaderosa@unina.it (G.R.).

ABSTRACT
In this study we aimed to confirm the emerging role of Chromatin Assembly Factor 1 (CAF-1 p60) as a new proliferation and prognostic marker for cancer and to test the usefulness of the tissue microarray technique (TMA) for CAF-1 p60 rapid screening in several human malignancies. CAF-1 is a histone chaperone, regulating chromatin dynamics during DNA replication and repair in eukaryotics. TMA is a powerful high-throughput methodology in the study of cancer, allowing simultaneous assessment of different biomarkers within large numbers of tissue specimens. We generated TMA taking 3 mm diameter-core biopsies from oral squamous cell carcinoma, prostate cancer, salivary gland tumours and skin melanoma specimens, which had been previously tested for CAF-1 p60 on routine tissue sections. We also analysed, for the first time, 30 larynx and 30 skin squamous cell carcinomas. CAF-1 p60 resulted over-expressed in both the tissue sections and the TMA specimens, with the highest levels of expression in tumours which were more aggressive and metastasizing. Notably, a high degree of agreement was found between the CAF-1 p60 assessment on TMAs and on routine tissue sections. Our findings confirm the prognostic role of CAF-1 p60 and indicate TMA as a really advantageous method for CAF-1 p60 immunohistochemical screening, allowing savings on both tissue quantity and operator-time.

Show MeSH
Related in: MedlinePlus