Limits...
Ultrasound-assisted extraction of carnosic acid and rosmarinic acid using ionic liquid solution from Rosmarinus officinalis.

Zu G, Zhang R, Yang L, Ma C, Zu Y, Wang W, Zhao C - Int J Mol Sci (2012)

Bottom Line: Ionic liquid based, ultrasound-assisted extraction was successfully applied to the extraction of phenolcarboxylic acids, carnosic acid and rosmarinic acid, from Rosmarinus officinalis.Ultrasound extraction parameters, including soaking time, solid-liquid ratio, ultrasound power and time, and the number of extraction cycles, were discussed by single factor experiments and the main influence factors were optimized by response surface methodology.The proposed approach was demonstrated as having higher efficiency, shorter extraction time and as a new alternative for the extraction of carnosic acid and rosmarinic acid from R. officinalis compared with traditional reference extraction methods.

View Article: PubMed Central - PubMed

Affiliation: State Engineering Laboratory for Bioresource Eco-Utilization, Northeast Forestry University, Harbin 150040, China; E-Mails: zygenefu@163.com (G.Z.); zhangrongrui1986@126.com (R.Z.); mchmchmchmch@163.com (C.M.); wjwang225@hotmail.com (W.W.); zcjsj@163.com (C.Z.).

ABSTRACT
Ionic liquid based, ultrasound-assisted extraction was successfully applied to the extraction of phenolcarboxylic acids, carnosic acid and rosmarinic acid, from Rosmarinus officinalis. Eight ionic liquids, with different cations and anions, were investigated in this work and [C(8)mim]Br was selected as the optimal solvent. Ultrasound extraction parameters, including soaking time, solid-liquid ratio, ultrasound power and time, and the number of extraction cycles, were discussed by single factor experiments and the main influence factors were optimized by response surface methodology. The proposed approach was demonstrated as having higher efficiency, shorter extraction time and as a new alternative for the extraction of carnosic acid and rosmarinic acid from R. officinalis compared with traditional reference extraction methods. Ionic liquids are considered to be green solvents, in the ultrasound-assisted extraction of key chemicals from medicinal plants, and show great potential.

Show MeSH
High-performance liquid chromatography (HPLC) profile of target compounds in an extract obtained using 1.0 M [C8mim]Br as extraction solvent. Inset: chemical structures of the target compounds.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC3472728&req=5

f4-ijms-13-11027: High-performance liquid chromatography (HPLC) profile of target compounds in an extract obtained using 1.0 M [C8mim]Br as extraction solvent. Inset: chemical structures of the target compounds.

Mentions: To evaluate the proposed ILUAE approach, some parameters such as linearity, reproducibility, stability, and recovery were determined under the above optimized conditions. Calibration curves were obtained by dissolving the standard of carnosic acid to mobile phase at seven concentrations in the range of 0.1–20.0 mg/mL under the same HPLC conditions for R. officinalis extraction. Linear regression equation and correlation coefficients is Yca = 2657149.4X − 202756.2 and 0.999, respectively. The limit of detection (LOD) and the limit of quantification (LOQ) were 6.23 μg/mL and 22.65 μg/mL, respectively. The linearity plotting was Yra = 20470465.5X + 1625134.1 (R2 = 0.998) over the concentration range from 0.01 to 2.00 mg/mL, where X was rosmarinic acid concentration as mg/mL and Yra was the peak area. The limit of detection was 0.81 μg/mL which was evaluated on the basis of a signal-to-noise ratio of 3, and the limit of quantification was 2.59 μg/mL. The reproducibility study was carried out on three repeated extraction with all methods. A comparison of the chromatograms of the two phenolcarboxylic acids obtained from standard solutions with that contained in the 1.0 M [C8mim]Br extract is shown in Figure 4.


Ultrasound-assisted extraction of carnosic acid and rosmarinic acid using ionic liquid solution from Rosmarinus officinalis.

Zu G, Zhang R, Yang L, Ma C, Zu Y, Wang W, Zhao C - Int J Mol Sci (2012)

High-performance liquid chromatography (HPLC) profile of target compounds in an extract obtained using 1.0 M [C8mim]Br as extraction solvent. Inset: chemical structures of the target compounds.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3472728&req=5

f4-ijms-13-11027: High-performance liquid chromatography (HPLC) profile of target compounds in an extract obtained using 1.0 M [C8mim]Br as extraction solvent. Inset: chemical structures of the target compounds.
Mentions: To evaluate the proposed ILUAE approach, some parameters such as linearity, reproducibility, stability, and recovery were determined under the above optimized conditions. Calibration curves were obtained by dissolving the standard of carnosic acid to mobile phase at seven concentrations in the range of 0.1–20.0 mg/mL under the same HPLC conditions for R. officinalis extraction. Linear regression equation and correlation coefficients is Yca = 2657149.4X − 202756.2 and 0.999, respectively. The limit of detection (LOD) and the limit of quantification (LOQ) were 6.23 μg/mL and 22.65 μg/mL, respectively. The linearity plotting was Yra = 20470465.5X + 1625134.1 (R2 = 0.998) over the concentration range from 0.01 to 2.00 mg/mL, where X was rosmarinic acid concentration as mg/mL and Yra was the peak area. The limit of detection was 0.81 μg/mL which was evaluated on the basis of a signal-to-noise ratio of 3, and the limit of quantification was 2.59 μg/mL. The reproducibility study was carried out on three repeated extraction with all methods. A comparison of the chromatograms of the two phenolcarboxylic acids obtained from standard solutions with that contained in the 1.0 M [C8mim]Br extract is shown in Figure 4.

Bottom Line: Ionic liquid based, ultrasound-assisted extraction was successfully applied to the extraction of phenolcarboxylic acids, carnosic acid and rosmarinic acid, from Rosmarinus officinalis.Ultrasound extraction parameters, including soaking time, solid-liquid ratio, ultrasound power and time, and the number of extraction cycles, were discussed by single factor experiments and the main influence factors were optimized by response surface methodology.The proposed approach was demonstrated as having higher efficiency, shorter extraction time and as a new alternative for the extraction of carnosic acid and rosmarinic acid from R. officinalis compared with traditional reference extraction methods.

View Article: PubMed Central - PubMed

Affiliation: State Engineering Laboratory for Bioresource Eco-Utilization, Northeast Forestry University, Harbin 150040, China; E-Mails: zygenefu@163.com (G.Z.); zhangrongrui1986@126.com (R.Z.); mchmchmchmch@163.com (C.M.); wjwang225@hotmail.com (W.W.); zcjsj@163.com (C.Z.).

ABSTRACT
Ionic liquid based, ultrasound-assisted extraction was successfully applied to the extraction of phenolcarboxylic acids, carnosic acid and rosmarinic acid, from Rosmarinus officinalis. Eight ionic liquids, with different cations and anions, were investigated in this work and [C(8)mim]Br was selected as the optimal solvent. Ultrasound extraction parameters, including soaking time, solid-liquid ratio, ultrasound power and time, and the number of extraction cycles, were discussed by single factor experiments and the main influence factors were optimized by response surface methodology. The proposed approach was demonstrated as having higher efficiency, shorter extraction time and as a new alternative for the extraction of carnosic acid and rosmarinic acid from R. officinalis compared with traditional reference extraction methods. Ionic liquids are considered to be green solvents, in the ultrasound-assisted extraction of key chemicals from medicinal plants, and show great potential.

Show MeSH