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Ultrasound-assisted extraction of carnosic acid and rosmarinic acid using ionic liquid solution from Rosmarinus officinalis.

Zu G, Zhang R, Yang L, Ma C, Zu Y, Wang W, Zhao C - Int J Mol Sci (2012)

Bottom Line: Ionic liquid based, ultrasound-assisted extraction was successfully applied to the extraction of phenolcarboxylic acids, carnosic acid and rosmarinic acid, from Rosmarinus officinalis.Ultrasound extraction parameters, including soaking time, solid-liquid ratio, ultrasound power and time, and the number of extraction cycles, were discussed by single factor experiments and the main influence factors were optimized by response surface methodology.The proposed approach was demonstrated as having higher efficiency, shorter extraction time and as a new alternative for the extraction of carnosic acid and rosmarinic acid from R. officinalis compared with traditional reference extraction methods.

View Article: PubMed Central - PubMed

Affiliation: State Engineering Laboratory for Bioresource Eco-Utilization, Northeast Forestry University, Harbin 150040, China; E-Mails: zygenefu@163.com (G.Z.); zhangrongrui1986@126.com (R.Z.); mchmchmchmch@163.com (C.M.); wjwang225@hotmail.com (W.W.); zcjsj@163.com (C.Z.).

ABSTRACT
Ionic liquid based, ultrasound-assisted extraction was successfully applied to the extraction of phenolcarboxylic acids, carnosic acid and rosmarinic acid, from Rosmarinus officinalis. Eight ionic liquids, with different cations and anions, were investigated in this work and [C(8)mim]Br was selected as the optimal solvent. Ultrasound extraction parameters, including soaking time, solid-liquid ratio, ultrasound power and time, and the number of extraction cycles, were discussed by single factor experiments and the main influence factors were optimized by response surface methodology. The proposed approach was demonstrated as having higher efficiency, shorter extraction time and as a new alternative for the extraction of carnosic acid and rosmarinic acid from R. officinalis compared with traditional reference extraction methods. Ionic liquids are considered to be green solvents, in the ultrasound-assisted extraction of key chemicals from medicinal plants, and show great potential.

Show MeSH
Effect of soaking time (a) solid–liquid ratio; (b) ultrasound power; (c) and ultrasound time; (d) on the extraction efficiency of target compounds with 1.0 M [C8mim]Br. The extraction efficiency is expressed as the observed values of target compounds and the maximum amount in each curve was taken to be 100%.
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f2-ijms-13-11027: Effect of soaking time (a) solid–liquid ratio; (b) ultrasound power; (c) and ultrasound time; (d) on the extraction efficiency of target compounds with 1.0 M [C8mim]Br. The extraction efficiency is expressed as the observed values of target compounds and the maximum amount in each curve was taken to be 100%.

Mentions: Experiments were conducted by soaking the dry herb powder in the ionic liquid solution for 1, 2, 3, 4, 8 h before UAE. Figure 2a shows the effect of soaking the herb in 1.0 M [C8mim]Br on the extraction of carnosic acid and rosmarinic acid from R. officinalis at room temperature (approx. 25 °C). It demonstrates the substantial increase in extraction efficiency obtained after soaking the herb. To extract phenolcarboxylic acids from the cellular structure, the solvent must have access to the cellular compartments where the phenolcarboxylic acids are located. An intact cell structure restricts accessibility of the solvent to the phenolcarboxylic acids, while ultrasound treated cells have a more open, fragmented structure, which facilitates efficient extraction. The increase in extraction efficiency of the phenolcarboxylic acids after soaking with the solvent is probably because of increased diffusion of the solvent into the cellular structure allowing improved solubilization of the phenolcarboxylic acids. The phenolcarboxylic acid extraction efficiency increased significantly when the soaking time was 0–2 h, however longer soaking times did not lead to further increases in efficiency. Hence 2 h was chosen as the optimal soaking time.


Ultrasound-assisted extraction of carnosic acid and rosmarinic acid using ionic liquid solution from Rosmarinus officinalis.

Zu G, Zhang R, Yang L, Ma C, Zu Y, Wang W, Zhao C - Int J Mol Sci (2012)

Effect of soaking time (a) solid–liquid ratio; (b) ultrasound power; (c) and ultrasound time; (d) on the extraction efficiency of target compounds with 1.0 M [C8mim]Br. The extraction efficiency is expressed as the observed values of target compounds and the maximum amount in each curve was taken to be 100%.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3472728&req=5

f2-ijms-13-11027: Effect of soaking time (a) solid–liquid ratio; (b) ultrasound power; (c) and ultrasound time; (d) on the extraction efficiency of target compounds with 1.0 M [C8mim]Br. The extraction efficiency is expressed as the observed values of target compounds and the maximum amount in each curve was taken to be 100%.
Mentions: Experiments were conducted by soaking the dry herb powder in the ionic liquid solution for 1, 2, 3, 4, 8 h before UAE. Figure 2a shows the effect of soaking the herb in 1.0 M [C8mim]Br on the extraction of carnosic acid and rosmarinic acid from R. officinalis at room temperature (approx. 25 °C). It demonstrates the substantial increase in extraction efficiency obtained after soaking the herb. To extract phenolcarboxylic acids from the cellular structure, the solvent must have access to the cellular compartments where the phenolcarboxylic acids are located. An intact cell structure restricts accessibility of the solvent to the phenolcarboxylic acids, while ultrasound treated cells have a more open, fragmented structure, which facilitates efficient extraction. The increase in extraction efficiency of the phenolcarboxylic acids after soaking with the solvent is probably because of increased diffusion of the solvent into the cellular structure allowing improved solubilization of the phenolcarboxylic acids. The phenolcarboxylic acid extraction efficiency increased significantly when the soaking time was 0–2 h, however longer soaking times did not lead to further increases in efficiency. Hence 2 h was chosen as the optimal soaking time.

Bottom Line: Ionic liquid based, ultrasound-assisted extraction was successfully applied to the extraction of phenolcarboxylic acids, carnosic acid and rosmarinic acid, from Rosmarinus officinalis.Ultrasound extraction parameters, including soaking time, solid-liquid ratio, ultrasound power and time, and the number of extraction cycles, were discussed by single factor experiments and the main influence factors were optimized by response surface methodology.The proposed approach was demonstrated as having higher efficiency, shorter extraction time and as a new alternative for the extraction of carnosic acid and rosmarinic acid from R. officinalis compared with traditional reference extraction methods.

View Article: PubMed Central - PubMed

Affiliation: State Engineering Laboratory for Bioresource Eco-Utilization, Northeast Forestry University, Harbin 150040, China; E-Mails: zygenefu@163.com (G.Z.); zhangrongrui1986@126.com (R.Z.); mchmchmchmch@163.com (C.M.); wjwang225@hotmail.com (W.W.); zcjsj@163.com (C.Z.).

ABSTRACT
Ionic liquid based, ultrasound-assisted extraction was successfully applied to the extraction of phenolcarboxylic acids, carnosic acid and rosmarinic acid, from Rosmarinus officinalis. Eight ionic liquids, with different cations and anions, were investigated in this work and [C(8)mim]Br was selected as the optimal solvent. Ultrasound extraction parameters, including soaking time, solid-liquid ratio, ultrasound power and time, and the number of extraction cycles, were discussed by single factor experiments and the main influence factors were optimized by response surface methodology. The proposed approach was demonstrated as having higher efficiency, shorter extraction time and as a new alternative for the extraction of carnosic acid and rosmarinic acid from R. officinalis compared with traditional reference extraction methods. Ionic liquids are considered to be green solvents, in the ultrasound-assisted extraction of key chemicals from medicinal plants, and show great potential.

Show MeSH