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Clinicopathological and prognostic significance of epithelial mesenchymal transition-related protein expression in intrahepatic cholangiocarcinoma.

Yao X, Wang X, Wang Z, Dai L, Zhang G, Yan Q, Zhou W - Onco Targets Ther (2012)

Bottom Line: The clinicopathological and prognostic value of these markers was also evaluated.Altered expression of EMT markers was associated with aggressive tumor behavior, including lymph node metastasis, undifferentiated-type histology, advanced tumor stage, venous invasion, and shorter overall survival.Our results suggest that the EMT process is associated with tumor progression and a poor outcome in patients with intrahepatic cholangiocarcinoma, and inhibition of EMT might offer novel promising molecular targets for the treatment of affected patients.

View Article: PubMed Central - PubMed

Affiliation: Huzhou Central Hospital, Zhejiang Huzhou.

ABSTRACT

Background: The aim of this study was to examine the patterns of expression of epithelial-mesenchymal transition (EMT)-related proteins in intrahepatic cholangiocarcinoma. The clinicopathological and prognostic value of these markers was also evaluated.

Methods: We detected the expression status of three EMT-related proteins, ie, E-cadherin, vimentin, and N-cadherin, by immunohistochemistry in consecutive intrahepatic cholangiocarcinoma specimens from 96 patients.

Results: The frequency of loss of the epithelial marker E-cadherin, and acquisition of mesenchymal markers, vimentin and N-cadherin, in intrahepatic cholangiocarcinoma was 43.8%, 37.5% and 57.3%, respectively. Altered expression of EMT markers was associated with aggressive tumor behavior, including lymph node metastasis, undifferentiated-type histology, advanced tumor stage, venous invasion, and shorter overall survival. Moreover, loss of E-cadherin was retained as an independent prognostic factor for patients with intrahepatic cholangiocarcinoma in multivariate analysis.

Conclusion: Our results suggest that the EMT process is associated with tumor progression and a poor outcome in patients with intrahepatic cholangiocarcinoma, and inhibition of EMT might offer novel promising molecular targets for the treatment of affected patients.

No MeSH data available.


Related in: MedlinePlus

Survival analysis of three epithelial-mesenchymal transition markers in univariate analysis using the Kaplan-Meier method determined by the log-rank test. E-cadherin loss (A) and upregulation of mesenchymal proteins vimentin (B) and N-cadherin (C) were found to be significantly associated with a poor outcome. There was also a trend toward coexistence of multiple epithelial-mesenchymal transition markers (D and E) associated with much more shorter overall survival.
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f2-ott-5-255: Survival analysis of three epithelial-mesenchymal transition markers in univariate analysis using the Kaplan-Meier method determined by the log-rank test. E-cadherin loss (A) and upregulation of mesenchymal proteins vimentin (B) and N-cadherin (C) were found to be significantly associated with a poor outcome. There was also a trend toward coexistence of multiple epithelial-mesenchymal transition markers (D and E) associated with much more shorter overall survival.

Mentions: Overall survival was determined using the log-rank test with respect to expression of the three EMT markers (Figure 2). In terms of the epithelial marker, E-cadherin, loss was found to be significantly associated with a poor outcome. This was also true for upregulation of the mesenchymal proteins, vimentin and N-cadherin. The three EMT markers and four other statistically significant prognostic factors (lymph node metastasis, venous invasion, pTNM stage, and histology) identified in univariate analysis were entered in the multivariate analysis. Among these factors, loss of E-cadherin expression and lymph node metastasis were shown to be independent prognostic indicators in patients with intrahepatic cholangiocarcinoma. However, vimentin and N-cadherin expression had no prognostic significance in multivariate analysis (Table 2).


Clinicopathological and prognostic significance of epithelial mesenchymal transition-related protein expression in intrahepatic cholangiocarcinoma.

Yao X, Wang X, Wang Z, Dai L, Zhang G, Yan Q, Zhou W - Onco Targets Ther (2012)

Survival analysis of three epithelial-mesenchymal transition markers in univariate analysis using the Kaplan-Meier method determined by the log-rank test. E-cadherin loss (A) and upregulation of mesenchymal proteins vimentin (B) and N-cadherin (C) were found to be significantly associated with a poor outcome. There was also a trend toward coexistence of multiple epithelial-mesenchymal transition markers (D and E) associated with much more shorter overall survival.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3472698&req=5

f2-ott-5-255: Survival analysis of three epithelial-mesenchymal transition markers in univariate analysis using the Kaplan-Meier method determined by the log-rank test. E-cadherin loss (A) and upregulation of mesenchymal proteins vimentin (B) and N-cadherin (C) were found to be significantly associated with a poor outcome. There was also a trend toward coexistence of multiple epithelial-mesenchymal transition markers (D and E) associated with much more shorter overall survival.
Mentions: Overall survival was determined using the log-rank test with respect to expression of the three EMT markers (Figure 2). In terms of the epithelial marker, E-cadherin, loss was found to be significantly associated with a poor outcome. This was also true for upregulation of the mesenchymal proteins, vimentin and N-cadherin. The three EMT markers and four other statistically significant prognostic factors (lymph node metastasis, venous invasion, pTNM stage, and histology) identified in univariate analysis were entered in the multivariate analysis. Among these factors, loss of E-cadherin expression and lymph node metastasis were shown to be independent prognostic indicators in patients with intrahepatic cholangiocarcinoma. However, vimentin and N-cadherin expression had no prognostic significance in multivariate analysis (Table 2).

Bottom Line: The clinicopathological and prognostic value of these markers was also evaluated.Altered expression of EMT markers was associated with aggressive tumor behavior, including lymph node metastasis, undifferentiated-type histology, advanced tumor stage, venous invasion, and shorter overall survival.Our results suggest that the EMT process is associated with tumor progression and a poor outcome in patients with intrahepatic cholangiocarcinoma, and inhibition of EMT might offer novel promising molecular targets for the treatment of affected patients.

View Article: PubMed Central - PubMed

Affiliation: Huzhou Central Hospital, Zhejiang Huzhou.

ABSTRACT

Background: The aim of this study was to examine the patterns of expression of epithelial-mesenchymal transition (EMT)-related proteins in intrahepatic cholangiocarcinoma. The clinicopathological and prognostic value of these markers was also evaluated.

Methods: We detected the expression status of three EMT-related proteins, ie, E-cadherin, vimentin, and N-cadherin, by immunohistochemistry in consecutive intrahepatic cholangiocarcinoma specimens from 96 patients.

Results: The frequency of loss of the epithelial marker E-cadherin, and acquisition of mesenchymal markers, vimentin and N-cadherin, in intrahepatic cholangiocarcinoma was 43.8%, 37.5% and 57.3%, respectively. Altered expression of EMT markers was associated with aggressive tumor behavior, including lymph node metastasis, undifferentiated-type histology, advanced tumor stage, venous invasion, and shorter overall survival. Moreover, loss of E-cadherin was retained as an independent prognostic factor for patients with intrahepatic cholangiocarcinoma in multivariate analysis.

Conclusion: Our results suggest that the EMT process is associated with tumor progression and a poor outcome in patients with intrahepatic cholangiocarcinoma, and inhibition of EMT might offer novel promising molecular targets for the treatment of affected patients.

No MeSH data available.


Related in: MedlinePlus