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Clinicopathological and prognostic significance of epithelial mesenchymal transition-related protein expression in intrahepatic cholangiocarcinoma.

Yao X, Wang X, Wang Z, Dai L, Zhang G, Yan Q, Zhou W - Onco Targets Ther (2012)

Bottom Line: The clinicopathological and prognostic value of these markers was also evaluated.Altered expression of EMT markers was associated with aggressive tumor behavior, including lymph node metastasis, undifferentiated-type histology, advanced tumor stage, venous invasion, and shorter overall survival.Our results suggest that the EMT process is associated with tumor progression and a poor outcome in patients with intrahepatic cholangiocarcinoma, and inhibition of EMT might offer novel promising molecular targets for the treatment of affected patients.

View Article: PubMed Central - PubMed

Affiliation: Huzhou Central Hospital, Zhejiang Huzhou.

ABSTRACT

Background: The aim of this study was to examine the patterns of expression of epithelial-mesenchymal transition (EMT)-related proteins in intrahepatic cholangiocarcinoma. The clinicopathological and prognostic value of these markers was also evaluated.

Methods: We detected the expression status of three EMT-related proteins, ie, E-cadherin, vimentin, and N-cadherin, by immunohistochemistry in consecutive intrahepatic cholangiocarcinoma specimens from 96 patients.

Results: The frequency of loss of the epithelial marker E-cadherin, and acquisition of mesenchymal markers, vimentin and N-cadherin, in intrahepatic cholangiocarcinoma was 43.8%, 37.5% and 57.3%, respectively. Altered expression of EMT markers was associated with aggressive tumor behavior, including lymph node metastasis, undifferentiated-type histology, advanced tumor stage, venous invasion, and shorter overall survival. Moreover, loss of E-cadherin was retained as an independent prognostic factor for patients with intrahepatic cholangiocarcinoma in multivariate analysis.

Conclusion: Our results suggest that the EMT process is associated with tumor progression and a poor outcome in patients with intrahepatic cholangiocarcinoma, and inhibition of EMT might offer novel promising molecular targets for the treatment of affected patients.

No MeSH data available.


Related in: MedlinePlus

Representative immunohistochemical images of three epithelial-mesenchymal transition markers in intrahepatic cholangiocarcinoma. (A) E-cadherin was strongly expressed in the plasma membrane of the tumor cells; (B) demonstration of loss of membranous E-cadherin in tumor cells; (C) negative N-cadherin expression with a positivity less than 10%; (D) demonstration of N-cadherin in the membrane and cytoplasm of tumor cells; (E) negative expression of vimentin in cancer cells while stromal cells were positively stained; and (F) acquired vimentin expression in cancer cells.
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f1-ott-5-255: Representative immunohistochemical images of three epithelial-mesenchymal transition markers in intrahepatic cholangiocarcinoma. (A) E-cadherin was strongly expressed in the plasma membrane of the tumor cells; (B) demonstration of loss of membranous E-cadherin in tumor cells; (C) negative N-cadherin expression with a positivity less than 10%; (D) demonstration of N-cadherin in the membrane and cytoplasm of tumor cells; (E) negative expression of vimentin in cancer cells while stromal cells were positively stained; and (F) acquired vimentin expression in cancer cells.

Mentions: The clinical and pathological characteristics of the patients are shown in Table 1. Immunohistochemical staining was performed to analyze the protein expression of the epithelial protein marker, E-cadherin, and the mesenchymal markers, vimentin and N-cadherin, in tumor specimens. Representative immunohistochemical results are shown in Figure 1. Epithelial E-cadherin protein loss frequency was observed in 43.8% (42/96) of tumors, and acquired mesenchymal vimentin and N-cadherin protein overexpression frequency was seen in 37.5% (36/96) and 57.3% (55/96) of tumors, respectively.


Clinicopathological and prognostic significance of epithelial mesenchymal transition-related protein expression in intrahepatic cholangiocarcinoma.

Yao X, Wang X, Wang Z, Dai L, Zhang G, Yan Q, Zhou W - Onco Targets Ther (2012)

Representative immunohistochemical images of three epithelial-mesenchymal transition markers in intrahepatic cholangiocarcinoma. (A) E-cadherin was strongly expressed in the plasma membrane of the tumor cells; (B) demonstration of loss of membranous E-cadherin in tumor cells; (C) negative N-cadherin expression with a positivity less than 10%; (D) demonstration of N-cadherin in the membrane and cytoplasm of tumor cells; (E) negative expression of vimentin in cancer cells while stromal cells were positively stained; and (F) acquired vimentin expression in cancer cells.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3472698&req=5

f1-ott-5-255: Representative immunohistochemical images of three epithelial-mesenchymal transition markers in intrahepatic cholangiocarcinoma. (A) E-cadherin was strongly expressed in the plasma membrane of the tumor cells; (B) demonstration of loss of membranous E-cadherin in tumor cells; (C) negative N-cadherin expression with a positivity less than 10%; (D) demonstration of N-cadherin in the membrane and cytoplasm of tumor cells; (E) negative expression of vimentin in cancer cells while stromal cells were positively stained; and (F) acquired vimentin expression in cancer cells.
Mentions: The clinical and pathological characteristics of the patients are shown in Table 1. Immunohistochemical staining was performed to analyze the protein expression of the epithelial protein marker, E-cadherin, and the mesenchymal markers, vimentin and N-cadherin, in tumor specimens. Representative immunohistochemical results are shown in Figure 1. Epithelial E-cadherin protein loss frequency was observed in 43.8% (42/96) of tumors, and acquired mesenchymal vimentin and N-cadherin protein overexpression frequency was seen in 37.5% (36/96) and 57.3% (55/96) of tumors, respectively.

Bottom Line: The clinicopathological and prognostic value of these markers was also evaluated.Altered expression of EMT markers was associated with aggressive tumor behavior, including lymph node metastasis, undifferentiated-type histology, advanced tumor stage, venous invasion, and shorter overall survival.Our results suggest that the EMT process is associated with tumor progression and a poor outcome in patients with intrahepatic cholangiocarcinoma, and inhibition of EMT might offer novel promising molecular targets for the treatment of affected patients.

View Article: PubMed Central - PubMed

Affiliation: Huzhou Central Hospital, Zhejiang Huzhou.

ABSTRACT

Background: The aim of this study was to examine the patterns of expression of epithelial-mesenchymal transition (EMT)-related proteins in intrahepatic cholangiocarcinoma. The clinicopathological and prognostic value of these markers was also evaluated.

Methods: We detected the expression status of three EMT-related proteins, ie, E-cadherin, vimentin, and N-cadherin, by immunohistochemistry in consecutive intrahepatic cholangiocarcinoma specimens from 96 patients.

Results: The frequency of loss of the epithelial marker E-cadherin, and acquisition of mesenchymal markers, vimentin and N-cadherin, in intrahepatic cholangiocarcinoma was 43.8%, 37.5% and 57.3%, respectively. Altered expression of EMT markers was associated with aggressive tumor behavior, including lymph node metastasis, undifferentiated-type histology, advanced tumor stage, venous invasion, and shorter overall survival. Moreover, loss of E-cadherin was retained as an independent prognostic factor for patients with intrahepatic cholangiocarcinoma in multivariate analysis.

Conclusion: Our results suggest that the EMT process is associated with tumor progression and a poor outcome in patients with intrahepatic cholangiocarcinoma, and inhibition of EMT might offer novel promising molecular targets for the treatment of affected patients.

No MeSH data available.


Related in: MedlinePlus