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Toona sinensis Leaf Aqueous Extract Improves the Functions of Sperm and Testes via Regulating Testicular Proteins in Rats under Oxidative Stress.

Yu BC, Yu WJ, Huang CY, Chen YH, Tsai YC, Chang CC, Chang SJ - Evid Based Complement Alternat Med (2012)

Bottom Line: Toona sinensis leaf (TSL) is commonly used as a vegetable and in spice in Asia.Protein expressions in testes identified by proteomic analysis and verified by Western blot demonstrated that TSL-A not only downregulated the level of glutathione transferase mu6 (antioxidant system), heat shock protein 90 kDa-β (protein misfolding repairing system), cofilin 2 (spermatogenesis), and cyclophilin A (apoptosis) but also upregulated crease3-hydroxy-3-methylglutaryl-coenzyme A synthase 2 (steroidogenesis), heat shock glycoprotein 96, and pancreatic trypsin 1 (sperm-oocyte interaction).These results indicate that TSL-A promotes the functions of sperm and testes via regulating multiple testicular proteins in rats under oxidative stress, suggesting that TSL-A is a valuable functional food supplement to improve functions of sperm and testes for males under oxidative stress.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Sciences, National Cheng Kung University, No. 1, University Road, Tainan 701, Taiwan.

ABSTRACT
Toona sinensis leaf (TSL) is commonly used as a vegetable and in spice in Asia. In this study, feeding with aqueous extract of TSL (TSL-A) alleviated oxidative stress and recovered the motility and functions of sperm in rats under oxidative stress. Protein expressions in testes identified by proteomic analysis and verified by Western blot demonstrated that TSL-A not only downregulated the level of glutathione transferase mu6 (antioxidant system), heat shock protein 90 kDa-β (protein misfolding repairing system), cofilin 2 (spermatogenesis), and cyclophilin A (apoptosis) but also upregulated crease3-hydroxy-3-methylglutaryl-coenzyme A synthase 2 (steroidogenesis), heat shock glycoprotein 96, and pancreatic trypsin 1 (sperm-oocyte interaction). These results indicate that TSL-A promotes the functions of sperm and testes via regulating multiple testicular proteins in rats under oxidative stress, suggesting that TSL-A is a valuable functional food supplement to improve functions of sperm and testes for males under oxidative stress.

No MeSH data available.


Related in: MedlinePlus

Confirmation of identified protein expressions by Western blot. (a) Expressions of identified proteins in testes of rats under oxidative stress and fed with normal chow, TSL-A (13 mg/kg/day), and GA (100 mg/kg/day) for 8 weeks were analyzed by Western blot; (b) quantification of identified proteins. All values are mean ± SEM (n = 4). #P < 0.05 compared with control group. *P < 0.05 and **P < 0.01 compared with H2O2-treated groups.
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fig4: Confirmation of identified protein expressions by Western blot. (a) Expressions of identified proteins in testes of rats under oxidative stress and fed with normal chow, TSL-A (13 mg/kg/day), and GA (100 mg/kg/day) for 8 weeks were analyzed by Western blot; (b) quantification of identified proteins. All values are mean ± SEM (n = 4). #P < 0.05 compared with control group. *P < 0.05 and **P < 0.01 compared with H2O2-treated groups.

Mentions: The beneficial effects of vegetables have been proposed to be the synergistic combinations of phytochemicals in vegetables [27]. The major phytochemical in TSL-A, which is GA exhibiting its protection against oxidative damage [7], may contribute to the protective effects of TSL-A on the functions of sperm and testes. In this study, GA regulated protein expressions of HMG CoA synthase, pancreatic trypsin 1, HSP-1β, cofilin 2, and cyclophilin A by a similar trend of TSL-A (Figure 4). On the contrary, TSL-A-regulated gp96 and GST mu 6 protein expressions which were not affected by GA may be attributed to the other components besides GA in TSL-A (Figure 4). Therefore, our results demonstrated that the protection of TSL-A for testes and sperm functions in rats under oxidative stress was through the multiple bioactivities of various phytochemicals.


Toona sinensis Leaf Aqueous Extract Improves the Functions of Sperm and Testes via Regulating Testicular Proteins in Rats under Oxidative Stress.

Yu BC, Yu WJ, Huang CY, Chen YH, Tsai YC, Chang CC, Chang SJ - Evid Based Complement Alternat Med (2012)

Confirmation of identified protein expressions by Western blot. (a) Expressions of identified proteins in testes of rats under oxidative stress and fed with normal chow, TSL-A (13 mg/kg/day), and GA (100 mg/kg/day) for 8 weeks were analyzed by Western blot; (b) quantification of identified proteins. All values are mean ± SEM (n = 4). #P < 0.05 compared with control group. *P < 0.05 and **P < 0.01 compared with H2O2-treated groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3472612&req=5

fig4: Confirmation of identified protein expressions by Western blot. (a) Expressions of identified proteins in testes of rats under oxidative stress and fed with normal chow, TSL-A (13 mg/kg/day), and GA (100 mg/kg/day) for 8 weeks were analyzed by Western blot; (b) quantification of identified proteins. All values are mean ± SEM (n = 4). #P < 0.05 compared with control group. *P < 0.05 and **P < 0.01 compared with H2O2-treated groups.
Mentions: The beneficial effects of vegetables have been proposed to be the synergistic combinations of phytochemicals in vegetables [27]. The major phytochemical in TSL-A, which is GA exhibiting its protection against oxidative damage [7], may contribute to the protective effects of TSL-A on the functions of sperm and testes. In this study, GA regulated protein expressions of HMG CoA synthase, pancreatic trypsin 1, HSP-1β, cofilin 2, and cyclophilin A by a similar trend of TSL-A (Figure 4). On the contrary, TSL-A-regulated gp96 and GST mu 6 protein expressions which were not affected by GA may be attributed to the other components besides GA in TSL-A (Figure 4). Therefore, our results demonstrated that the protection of TSL-A for testes and sperm functions in rats under oxidative stress was through the multiple bioactivities of various phytochemicals.

Bottom Line: Toona sinensis leaf (TSL) is commonly used as a vegetable and in spice in Asia.Protein expressions in testes identified by proteomic analysis and verified by Western blot demonstrated that TSL-A not only downregulated the level of glutathione transferase mu6 (antioxidant system), heat shock protein 90 kDa-β (protein misfolding repairing system), cofilin 2 (spermatogenesis), and cyclophilin A (apoptosis) but also upregulated crease3-hydroxy-3-methylglutaryl-coenzyme A synthase 2 (steroidogenesis), heat shock glycoprotein 96, and pancreatic trypsin 1 (sperm-oocyte interaction).These results indicate that TSL-A promotes the functions of sperm and testes via regulating multiple testicular proteins in rats under oxidative stress, suggesting that TSL-A is a valuable functional food supplement to improve functions of sperm and testes for males under oxidative stress.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Sciences, National Cheng Kung University, No. 1, University Road, Tainan 701, Taiwan.

ABSTRACT
Toona sinensis leaf (TSL) is commonly used as a vegetable and in spice in Asia. In this study, feeding with aqueous extract of TSL (TSL-A) alleviated oxidative stress and recovered the motility and functions of sperm in rats under oxidative stress. Protein expressions in testes identified by proteomic analysis and verified by Western blot demonstrated that TSL-A not only downregulated the level of glutathione transferase mu6 (antioxidant system), heat shock protein 90 kDa-β (protein misfolding repairing system), cofilin 2 (spermatogenesis), and cyclophilin A (apoptosis) but also upregulated crease3-hydroxy-3-methylglutaryl-coenzyme A synthase 2 (steroidogenesis), heat shock glycoprotein 96, and pancreatic trypsin 1 (sperm-oocyte interaction). These results indicate that TSL-A promotes the functions of sperm and testes via regulating multiple testicular proteins in rats under oxidative stress, suggesting that TSL-A is a valuable functional food supplement to improve functions of sperm and testes for males under oxidative stress.

No MeSH data available.


Related in: MedlinePlus