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Toona sinensis Leaf Aqueous Extract Improves the Functions of Sperm and Testes via Regulating Testicular Proteins in Rats under Oxidative Stress.

Yu BC, Yu WJ, Huang CY, Chen YH, Tsai YC, Chang CC, Chang SJ - Evid Based Complement Alternat Med (2012)

Bottom Line: Toona sinensis leaf (TSL) is commonly used as a vegetable and in spice in Asia.Protein expressions in testes identified by proteomic analysis and verified by Western blot demonstrated that TSL-A not only downregulated the level of glutathione transferase mu6 (antioxidant system), heat shock protein 90 kDa-β (protein misfolding repairing system), cofilin 2 (spermatogenesis), and cyclophilin A (apoptosis) but also upregulated crease3-hydroxy-3-methylglutaryl-coenzyme A synthase 2 (steroidogenesis), heat shock glycoprotein 96, and pancreatic trypsin 1 (sperm-oocyte interaction).These results indicate that TSL-A promotes the functions of sperm and testes via regulating multiple testicular proteins in rats under oxidative stress, suggesting that TSL-A is a valuable functional food supplement to improve functions of sperm and testes for males under oxidative stress.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Sciences, National Cheng Kung University, No. 1, University Road, Tainan 701, Taiwan.

ABSTRACT
Toona sinensis leaf (TSL) is commonly used as a vegetable and in spice in Asia. In this study, feeding with aqueous extract of TSL (TSL-A) alleviated oxidative stress and recovered the motility and functions of sperm in rats under oxidative stress. Protein expressions in testes identified by proteomic analysis and verified by Western blot demonstrated that TSL-A not only downregulated the level of glutathione transferase mu6 (antioxidant system), heat shock protein 90 kDa-β (protein misfolding repairing system), cofilin 2 (spermatogenesis), and cyclophilin A (apoptosis) but also upregulated crease3-hydroxy-3-methylglutaryl-coenzyme A synthase 2 (steroidogenesis), heat shock glycoprotein 96, and pancreatic trypsin 1 (sperm-oocyte interaction). These results indicate that TSL-A promotes the functions of sperm and testes via regulating multiple testicular proteins in rats under oxidative stress, suggesting that TSL-A is a valuable functional food supplement to improve functions of sperm and testes for males under oxidative stress.

No MeSH data available.


Related in: MedlinePlus

Alterations of 7 identified protein expressions from 2-DE gel. Protein expressions in testes of SD rats IP injected with 1 mmol H2O2/kg b.w every other day and fed with normal diet and TSL-A (13 mg/kg b.w/day) for 8 weeks were separated by 2-DE. Protein spots with numbers represented the 7-protein expression more than 2-fold analyzed by the ImageMaster 2D Platinum Software. Details of the proteins were given in Table 1. The symbols “+” and “−” represent the upregulation and downregulation of the protein expression, respectively. The expression fold was that of the H2O2 group.
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fig3: Alterations of 7 identified protein expressions from 2-DE gel. Protein expressions in testes of SD rats IP injected with 1 mmol H2O2/kg b.w every other day and fed with normal diet and TSL-A (13 mg/kg b.w/day) for 8 weeks were separated by 2-DE. Protein spots with numbers represented the 7-protein expression more than 2-fold analyzed by the ImageMaster 2D Platinum Software. Details of the proteins were given in Table 1. The symbols “+” and “−” represent the upregulation and downregulation of the protein expression, respectively. The expression fold was that of the H2O2 group.

Mentions: Cofilin, a family of actin-binding proteins which disassemble actin filaments, is widely distributed throughout nonmuscle (cofilin 1) and muscle (cofilin 2) cells [18]. H2O2-induced abnormal cofilin aggregation in the mitochondria is suggested to contribute to the loss of MMP, failure of spermatogenesis, and spermatocytes apoptosis [18]. The elevated levels of sperm MMP by TSL-A (Figure 1(b)), in combined with alleviation of abnormal H2O2-induced cofilin 2 expressions by TSL-A (Figure 3) demonstrated that TSL-A prevented the abnormal aggregation of cofilin 2, maintained the sperm MMP and spermatogenesis, and inhibited the cell apoptosis in testes of rats under oxidative stress.


Toona sinensis Leaf Aqueous Extract Improves the Functions of Sperm and Testes via Regulating Testicular Proteins in Rats under Oxidative Stress.

Yu BC, Yu WJ, Huang CY, Chen YH, Tsai YC, Chang CC, Chang SJ - Evid Based Complement Alternat Med (2012)

Alterations of 7 identified protein expressions from 2-DE gel. Protein expressions in testes of SD rats IP injected with 1 mmol H2O2/kg b.w every other day and fed with normal diet and TSL-A (13 mg/kg b.w/day) for 8 weeks were separated by 2-DE. Protein spots with numbers represented the 7-protein expression more than 2-fold analyzed by the ImageMaster 2D Platinum Software. Details of the proteins were given in Table 1. The symbols “+” and “−” represent the upregulation and downregulation of the protein expression, respectively. The expression fold was that of the H2O2 group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3472612&req=5

fig3: Alterations of 7 identified protein expressions from 2-DE gel. Protein expressions in testes of SD rats IP injected with 1 mmol H2O2/kg b.w every other day and fed with normal diet and TSL-A (13 mg/kg b.w/day) for 8 weeks were separated by 2-DE. Protein spots with numbers represented the 7-protein expression more than 2-fold analyzed by the ImageMaster 2D Platinum Software. Details of the proteins were given in Table 1. The symbols “+” and “−” represent the upregulation and downregulation of the protein expression, respectively. The expression fold was that of the H2O2 group.
Mentions: Cofilin, a family of actin-binding proteins which disassemble actin filaments, is widely distributed throughout nonmuscle (cofilin 1) and muscle (cofilin 2) cells [18]. H2O2-induced abnormal cofilin aggregation in the mitochondria is suggested to contribute to the loss of MMP, failure of spermatogenesis, and spermatocytes apoptosis [18]. The elevated levels of sperm MMP by TSL-A (Figure 1(b)), in combined with alleviation of abnormal H2O2-induced cofilin 2 expressions by TSL-A (Figure 3) demonstrated that TSL-A prevented the abnormal aggregation of cofilin 2, maintained the sperm MMP and spermatogenesis, and inhibited the cell apoptosis in testes of rats under oxidative stress.

Bottom Line: Toona sinensis leaf (TSL) is commonly used as a vegetable and in spice in Asia.Protein expressions in testes identified by proteomic analysis and verified by Western blot demonstrated that TSL-A not only downregulated the level of glutathione transferase mu6 (antioxidant system), heat shock protein 90 kDa-β (protein misfolding repairing system), cofilin 2 (spermatogenesis), and cyclophilin A (apoptosis) but also upregulated crease3-hydroxy-3-methylglutaryl-coenzyme A synthase 2 (steroidogenesis), heat shock glycoprotein 96, and pancreatic trypsin 1 (sperm-oocyte interaction).These results indicate that TSL-A promotes the functions of sperm and testes via regulating multiple testicular proteins in rats under oxidative stress, suggesting that TSL-A is a valuable functional food supplement to improve functions of sperm and testes for males under oxidative stress.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Sciences, National Cheng Kung University, No. 1, University Road, Tainan 701, Taiwan.

ABSTRACT
Toona sinensis leaf (TSL) is commonly used as a vegetable and in spice in Asia. In this study, feeding with aqueous extract of TSL (TSL-A) alleviated oxidative stress and recovered the motility and functions of sperm in rats under oxidative stress. Protein expressions in testes identified by proteomic analysis and verified by Western blot demonstrated that TSL-A not only downregulated the level of glutathione transferase mu6 (antioxidant system), heat shock protein 90 kDa-β (protein misfolding repairing system), cofilin 2 (spermatogenesis), and cyclophilin A (apoptosis) but also upregulated crease3-hydroxy-3-methylglutaryl-coenzyme A synthase 2 (steroidogenesis), heat shock glycoprotein 96, and pancreatic trypsin 1 (sperm-oocyte interaction). These results indicate that TSL-A promotes the functions of sperm and testes via regulating multiple testicular proteins in rats under oxidative stress, suggesting that TSL-A is a valuable functional food supplement to improve functions of sperm and testes for males under oxidative stress.

No MeSH data available.


Related in: MedlinePlus