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Toona sinensis Leaf Aqueous Extract Improves the Functions of Sperm and Testes via Regulating Testicular Proteins in Rats under Oxidative Stress.

Yu BC, Yu WJ, Huang CY, Chen YH, Tsai YC, Chang CC, Chang SJ - Evid Based Complement Alternat Med (2012)

Bottom Line: Toona sinensis leaf (TSL) is commonly used as a vegetable and in spice in Asia.Protein expressions in testes identified by proteomic analysis and verified by Western blot demonstrated that TSL-A not only downregulated the level of glutathione transferase mu6 (antioxidant system), heat shock protein 90 kDa-β (protein misfolding repairing system), cofilin 2 (spermatogenesis), and cyclophilin A (apoptosis) but also upregulated crease3-hydroxy-3-methylglutaryl-coenzyme A synthase 2 (steroidogenesis), heat shock glycoprotein 96, and pancreatic trypsin 1 (sperm-oocyte interaction).These results indicate that TSL-A promotes the functions of sperm and testes via regulating multiple testicular proteins in rats under oxidative stress, suggesting that TSL-A is a valuable functional food supplement to improve functions of sperm and testes for males under oxidative stress.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Sciences, National Cheng Kung University, No. 1, University Road, Tainan 701, Taiwan.

ABSTRACT
Toona sinensis leaf (TSL) is commonly used as a vegetable and in spice in Asia. In this study, feeding with aqueous extract of TSL (TSL-A) alleviated oxidative stress and recovered the motility and functions of sperm in rats under oxidative stress. Protein expressions in testes identified by proteomic analysis and verified by Western blot demonstrated that TSL-A not only downregulated the level of glutathione transferase mu6 (antioxidant system), heat shock protein 90 kDa-β (protein misfolding repairing system), cofilin 2 (spermatogenesis), and cyclophilin A (apoptosis) but also upregulated crease3-hydroxy-3-methylglutaryl-coenzyme A synthase 2 (steroidogenesis), heat shock glycoprotein 96, and pancreatic trypsin 1 (sperm-oocyte interaction). These results indicate that TSL-A promotes the functions of sperm and testes via regulating multiple testicular proteins in rats under oxidative stress, suggesting that TSL-A is a valuable functional food supplement to improve functions of sperm and testes for males under oxidative stress.

No MeSH data available.


Related in: MedlinePlus

Changes of the levels of sperm ROS, MMP, and motility in rats under oxidative stress without and with TSL-A or GA feeding. (a) Change of ROS level in sperm. Sperms from different group were incubated with DCFH2-DA as an indicator for ROS; (b) change of the MMP level in sperm. Sperms from different group were incubated with DiCO6 as an indicator for MMP; (c) sperm motility of different group. Rats were randomly divided into five groups: vehicle group, H2O2 group, the H2O2 treatment plus TSL-A feeding (TSL-A), H2O2 treatment, and H2O2 treatment plus GA feeding (GA). Both the ROS and MMP levels of sperm were measured by flow cytometry. The sperm motility was determined by microscope. The symbols “→” and “←” represent the increased and decreased ROS and MMP level compared with that of vehicle group, respectively. *P < 0.05 compared with vehicle group.
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fig1: Changes of the levels of sperm ROS, MMP, and motility in rats under oxidative stress without and with TSL-A or GA feeding. (a) Change of ROS level in sperm. Sperms from different group were incubated with DCFH2-DA as an indicator for ROS; (b) change of the MMP level in sperm. Sperms from different group were incubated with DiCO6 as an indicator for MMP; (c) sperm motility of different group. Rats were randomly divided into five groups: vehicle group, H2O2 group, the H2O2 treatment plus TSL-A feeding (TSL-A), H2O2 treatment, and H2O2 treatment plus GA feeding (GA). Both the ROS and MMP levels of sperm were measured by flow cytometry. The sperm motility was determined by microscope. The symbols “→” and “←” represent the increased and decreased ROS and MMP level compared with that of vehicle group, respectively. *P < 0.05 compared with vehicle group.

Mentions: Administration with TSL-A and GA decreased ROS (Figure 1(a)), increased MMP (Figure 1(b)), and restored sperm motility (Figure 1(c)) in sperms of rats under oxidative stress. Seven testicular proteins detected by proteomic analysis were regulated by TSL-A, including glutathione transferase mu6 (GST mu6), heat shock protein 90 kDa-β (HSP-1β), 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 (HMG CoA synthase), pancreatic trypsin 1, heat shock glycoprotein 96 (gp96), cofilin 2, and cyclophilin A (Table 1 and Figure 2). Results of Western blot indicated that the levels of proteins (gp96, HMG-CoA synthase, and pancreatic trypsin 1) which decreased by H2O2 were found to be significantly increased by TSL-A when compared with normal group (Figure 2(b)). In contrast, proteins (GST mu6, HSP-1β, cofilin 2, and cyclophilin A) whose levels increased by H2O2 were significantly decreased by TSL-A when compared with normal group (Figure 2(b)). GA decreased levels of HSP-1β, cofilin 2, and cyclophilin A that were also significantly decreased by TSL-A. Among those proteins (gp96, HMG-CoA synthase, and pancreatic trypsin 1) whose levels were significantly increased by TSL-A, GA significantly increased level of HMG-CoA synthase and pancreatic trypsin 1 (Figure 2(b)).


Toona sinensis Leaf Aqueous Extract Improves the Functions of Sperm and Testes via Regulating Testicular Proteins in Rats under Oxidative Stress.

Yu BC, Yu WJ, Huang CY, Chen YH, Tsai YC, Chang CC, Chang SJ - Evid Based Complement Alternat Med (2012)

Changes of the levels of sperm ROS, MMP, and motility in rats under oxidative stress without and with TSL-A or GA feeding. (a) Change of ROS level in sperm. Sperms from different group were incubated with DCFH2-DA as an indicator for ROS; (b) change of the MMP level in sperm. Sperms from different group were incubated with DiCO6 as an indicator for MMP; (c) sperm motility of different group. Rats were randomly divided into five groups: vehicle group, H2O2 group, the H2O2 treatment plus TSL-A feeding (TSL-A), H2O2 treatment, and H2O2 treatment plus GA feeding (GA). Both the ROS and MMP levels of sperm were measured by flow cytometry. The sperm motility was determined by microscope. The symbols “→” and “←” represent the increased and decreased ROS and MMP level compared with that of vehicle group, respectively. *P < 0.05 compared with vehicle group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3472612&req=5

fig1: Changes of the levels of sperm ROS, MMP, and motility in rats under oxidative stress without and with TSL-A or GA feeding. (a) Change of ROS level in sperm. Sperms from different group were incubated with DCFH2-DA as an indicator for ROS; (b) change of the MMP level in sperm. Sperms from different group were incubated with DiCO6 as an indicator for MMP; (c) sperm motility of different group. Rats were randomly divided into five groups: vehicle group, H2O2 group, the H2O2 treatment plus TSL-A feeding (TSL-A), H2O2 treatment, and H2O2 treatment plus GA feeding (GA). Both the ROS and MMP levels of sperm were measured by flow cytometry. The sperm motility was determined by microscope. The symbols “→” and “←” represent the increased and decreased ROS and MMP level compared with that of vehicle group, respectively. *P < 0.05 compared with vehicle group.
Mentions: Administration with TSL-A and GA decreased ROS (Figure 1(a)), increased MMP (Figure 1(b)), and restored sperm motility (Figure 1(c)) in sperms of rats under oxidative stress. Seven testicular proteins detected by proteomic analysis were regulated by TSL-A, including glutathione transferase mu6 (GST mu6), heat shock protein 90 kDa-β (HSP-1β), 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 (HMG CoA synthase), pancreatic trypsin 1, heat shock glycoprotein 96 (gp96), cofilin 2, and cyclophilin A (Table 1 and Figure 2). Results of Western blot indicated that the levels of proteins (gp96, HMG-CoA synthase, and pancreatic trypsin 1) which decreased by H2O2 were found to be significantly increased by TSL-A when compared with normal group (Figure 2(b)). In contrast, proteins (GST mu6, HSP-1β, cofilin 2, and cyclophilin A) whose levels increased by H2O2 were significantly decreased by TSL-A when compared with normal group (Figure 2(b)). GA decreased levels of HSP-1β, cofilin 2, and cyclophilin A that were also significantly decreased by TSL-A. Among those proteins (gp96, HMG-CoA synthase, and pancreatic trypsin 1) whose levels were significantly increased by TSL-A, GA significantly increased level of HMG-CoA synthase and pancreatic trypsin 1 (Figure 2(b)).

Bottom Line: Toona sinensis leaf (TSL) is commonly used as a vegetable and in spice in Asia.Protein expressions in testes identified by proteomic analysis and verified by Western blot demonstrated that TSL-A not only downregulated the level of glutathione transferase mu6 (antioxidant system), heat shock protein 90 kDa-β (protein misfolding repairing system), cofilin 2 (spermatogenesis), and cyclophilin A (apoptosis) but also upregulated crease3-hydroxy-3-methylglutaryl-coenzyme A synthase 2 (steroidogenesis), heat shock glycoprotein 96, and pancreatic trypsin 1 (sperm-oocyte interaction).These results indicate that TSL-A promotes the functions of sperm and testes via regulating multiple testicular proteins in rats under oxidative stress, suggesting that TSL-A is a valuable functional food supplement to improve functions of sperm and testes for males under oxidative stress.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Sciences, National Cheng Kung University, No. 1, University Road, Tainan 701, Taiwan.

ABSTRACT
Toona sinensis leaf (TSL) is commonly used as a vegetable and in spice in Asia. In this study, feeding with aqueous extract of TSL (TSL-A) alleviated oxidative stress and recovered the motility and functions of sperm in rats under oxidative stress. Protein expressions in testes identified by proteomic analysis and verified by Western blot demonstrated that TSL-A not only downregulated the level of glutathione transferase mu6 (antioxidant system), heat shock protein 90 kDa-β (protein misfolding repairing system), cofilin 2 (spermatogenesis), and cyclophilin A (apoptosis) but also upregulated crease3-hydroxy-3-methylglutaryl-coenzyme A synthase 2 (steroidogenesis), heat shock glycoprotein 96, and pancreatic trypsin 1 (sperm-oocyte interaction). These results indicate that TSL-A promotes the functions of sperm and testes via regulating multiple testicular proteins in rats under oxidative stress, suggesting that TSL-A is a valuable functional food supplement to improve functions of sperm and testes for males under oxidative stress.

No MeSH data available.


Related in: MedlinePlus