Limits...
Phylogenetic analysis of the Kinesin superfamily from physcomitrella.

Shen Z, Collatos AR, Bibeau JP, Furt F, Vidali L - Front Plant Sci (2012)

Bottom Line: We found a remarkable conservation of families and subfamily classes with Arabidopsis, which is important for future comparative analysis of function.Some of the families, such as kinesins 14s are composed of fewer members in moss, while other families, such as the kinesin 12s are greatly expanded.To improve the comparison between species, and to simplify communication between research groups, we propose a classification of subfamilies based on our phylogenetic analysis.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology and Biotechnology, Worcester Polytechnic Institute Worcester, MA, USA.

ABSTRACT
Kinesins are an ancient superfamily of microtubule dependent motors. They participate in an extensive and diverse list of essential cellular functions, including mitosis, cytokinesis, cell polarization, cell elongation, flagellar development, and intracellular transport. Based on phylogenetic relationships, the kinesin superfamily has been subdivided into 14 families, which are represented in most eukaryotic phyla. The functions of these families are sometimes conserved between species, but important variations in function across species have been observed. Plants possess most kinesin families including a few plant specific families. With the availability of an ever increasing number of genome sequences from plants, it is important to document the complete complement of kinesins present in a given organism. This will help develop a molecular framework to explore the function of each family using genetics, biochemistry, and cell biology. The moss Physcomitrella patens has emerged as a powerful model organism to study gene function in plants, which makes it a key candidate to explore complex gene families, such as the kinesin superfamily. Here we report a detailed phylogenetic characterization of the 71 kinesins of the kinesin superfamily in Physcomitrella. We found a remarkable conservation of families and subfamily classes with Arabidopsis, which is important for future comparative analysis of function. Some of the families, such as kinesins 14s are composed of fewer members in moss, while other families, such as the kinesin 12s are greatly expanded. To improve the comparison between species, and to simplify communication between research groups, we propose a classification of subfamilies based on our phylogenetic analysis.

No MeSH data available.


Related in: MedlinePlus

Gene models of (A) kinesin 8s and (B) kinesin 13s. Schematic diagrams showing the structure and domain architecture of kinesin 8s and 13s. Domains are indicated at the bottom of the diagrams.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3472504&req=5

Figure 8: Gene models of (A) kinesin 8s and (B) kinesin 13s. Schematic diagrams showing the structure and domain architecture of kinesin 8s and 13s. Domains are indicated at the bottom of the diagrams.

Mentions: Although nothing is known about kinesin 8s in plant systems, significant research has been conducted on these kinesins in animal and fungi. Some of the group’s functions include mitochondrial transport in Drosophila, mitotic chromosome segregation in yeast, and MT destabilization in humans (Miki et al., 2005; Peters et al., 2010). Our phylogenetic analysis indicates that there are two kinesin 8 classes for both moss and Arabidopsis (Figure 7). Of these two, class I contains a single Arabidopsis kinesin and two moss kinesins; class II contains a single moss and Arabidopsis kinesin. The gene model for the moss class II kinesin shows an extended N-terminal domain (Figure 8A). Because of their similarity to other kinesin 8 members from animals and fungi, we anticipate these kinesins will have a conserved function. Nevertheless, the existence of two ortholog genes in plants suggests that diversification of function was already present, to some degree, in the last common ancestor of bryophytes and vascular plants.


Phylogenetic analysis of the Kinesin superfamily from physcomitrella.

Shen Z, Collatos AR, Bibeau JP, Furt F, Vidali L - Front Plant Sci (2012)

Gene models of (A) kinesin 8s and (B) kinesin 13s. Schematic diagrams showing the structure and domain architecture of kinesin 8s and 13s. Domains are indicated at the bottom of the diagrams.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3472504&req=5

Figure 8: Gene models of (A) kinesin 8s and (B) kinesin 13s. Schematic diagrams showing the structure and domain architecture of kinesin 8s and 13s. Domains are indicated at the bottom of the diagrams.
Mentions: Although nothing is known about kinesin 8s in plant systems, significant research has been conducted on these kinesins in animal and fungi. Some of the group’s functions include mitochondrial transport in Drosophila, mitotic chromosome segregation in yeast, and MT destabilization in humans (Miki et al., 2005; Peters et al., 2010). Our phylogenetic analysis indicates that there are two kinesin 8 classes for both moss and Arabidopsis (Figure 7). Of these two, class I contains a single Arabidopsis kinesin and two moss kinesins; class II contains a single moss and Arabidopsis kinesin. The gene model for the moss class II kinesin shows an extended N-terminal domain (Figure 8A). Because of their similarity to other kinesin 8 members from animals and fungi, we anticipate these kinesins will have a conserved function. Nevertheless, the existence of two ortholog genes in plants suggests that diversification of function was already present, to some degree, in the last common ancestor of bryophytes and vascular plants.

Bottom Line: We found a remarkable conservation of families and subfamily classes with Arabidopsis, which is important for future comparative analysis of function.Some of the families, such as kinesins 14s are composed of fewer members in moss, while other families, such as the kinesin 12s are greatly expanded.To improve the comparison between species, and to simplify communication between research groups, we propose a classification of subfamilies based on our phylogenetic analysis.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology and Biotechnology, Worcester Polytechnic Institute Worcester, MA, USA.

ABSTRACT
Kinesins are an ancient superfamily of microtubule dependent motors. They participate in an extensive and diverse list of essential cellular functions, including mitosis, cytokinesis, cell polarization, cell elongation, flagellar development, and intracellular transport. Based on phylogenetic relationships, the kinesin superfamily has been subdivided into 14 families, which are represented in most eukaryotic phyla. The functions of these families are sometimes conserved between species, but important variations in function across species have been observed. Plants possess most kinesin families including a few plant specific families. With the availability of an ever increasing number of genome sequences from plants, it is important to document the complete complement of kinesins present in a given organism. This will help develop a molecular framework to explore the function of each family using genetics, biochemistry, and cell biology. The moss Physcomitrella patens has emerged as a powerful model organism to study gene function in plants, which makes it a key candidate to explore complex gene families, such as the kinesin superfamily. Here we report a detailed phylogenetic characterization of the 71 kinesins of the kinesin superfamily in Physcomitrella. We found a remarkable conservation of families and subfamily classes with Arabidopsis, which is important for future comparative analysis of function. Some of the families, such as kinesins 14s are composed of fewer members in moss, while other families, such as the kinesin 12s are greatly expanded. To improve the comparison between species, and to simplify communication between research groups, we propose a classification of subfamilies based on our phylogenetic analysis.

No MeSH data available.


Related in: MedlinePlus