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Evaluation of antioxidant and cytoprotective activities of Arnica montana L. and Artemisia absinthium L. ethanolic extracts.

Craciunescu O, Constantin D, Gaspar A, Toma L, Utoiu E, Moldovan L - Chem Cent J (2012)

Bottom Line: Both plant extracts had significant effects on the growth of NCTC cells in the range of 10-100 mg/L A. montana and 10-500 mg/L A. absinthium.A. montana and A. absinthium extracts, rich in flavonoids and phenolic acids, showed a good antioxidant activity and cytoprotective effect against oxidative damage in fibroblast-like cells.These results provide scientific support for the traditional use of A. montana and A. absinthium in treatment of skin disorders.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Cellular and Molecular Biology, National Institute of Research and Development for Biological Sciences, 296, Splaiul Independentei, 060031, Bucharest, Romania. moldovanlc@yahoo.com.

ABSTRACT

Background: Arnica montana L. and Artemisia absinthium L. (Asteraceae) are medicinal plants native to temperate regions of Europe, including Romania, traditionally used for treatment of skin wounds, bruises and contusions. In the present study, A. montana and A. absinthium ethanolic extracts were evaluated for their chemical composition, antioxidant activity and protective effect against H2O2-induced oxidative stress in a mouse fibroblast-like NCTC cell line.

Results: A. absinthium extract showed a higher antioxidant capacity than A. montana extract as Trolox equivalent antioxidant capacity, Oxygen radical absorbance capacity and 2,2-diphenyl-1-picrylhydrazyl free radical-scavenging activity, in correlation with its flavonoids and phenolic acids content. Both plant extracts had significant effects on the growth of NCTC cells in the range of 10-100 mg/L A. montana and 10-500 mg/L A. absinthium. They also protected fibroblast cells against hydrogen peroxide-induced oxidative damage, at the same doses. The best protection was observed in cell pre-treatment with 10 mg/L A. montana and 10-300 mg/L A. absinthium, respectively, as determined by Neutral red and lactate dehydrogenase assays. In addition, cell pre-treatment with plant extracts, at these concentrations, prevented morphological changes induced by hydrogen peroxide. Flow-cytometry analysis showed that pre-treatment with A. montana and A. absinthium extracts restored the proportion of cells in each phase of the cell cycle.

Conclusions: A. montana and A. absinthium extracts, rich in flavonoids and phenolic acids, showed a good antioxidant activity and cytoprotective effect against oxidative damage in fibroblast-like cells. These results provide scientific support for the traditional use of A. montana and A. absinthium in treatment of skin disorders.

No MeSH data available.


Related in: MedlinePlus

A Effect of plant extracts on cell cycle distribution of NCTC cells. Co-treated and pre-treated cells were stained with propidium iodide and analyzed by flow-cytometry. B DNA histograms analyzed by MODFITTM LT 3.0 software. Results are represented as mean ± SD (n = 6). Each phase of individual samples was compared with correspondent phase in untreated control using paired t-test. Statistically significant differences were observed (##p < 0.01).
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Figure 3: A Effect of plant extracts on cell cycle distribution of NCTC cells. Co-treated and pre-treated cells were stained with propidium iodide and analyzed by flow-cytometry. B DNA histograms analyzed by MODFITTM LT 3.0 software. Results are represented as mean ± SD (n = 6). Each phase of individual samples was compared with correspondent phase in untreated control using paired t-test. Statistically significant differences were observed (##p < 0.01).

Mentions: Cell cycle distribution was analyzed after treatment of H2O2-injured cells with 10 mg/L arnica and 300 mg/L wormwood, respectively. As shown in Figure 3A, H2O2-treated cells had an altered cycle that was arrested in G0/G1 and G2/M phases. The results showed a proportion of cells in G0/G1 phase that decreased from 60.61% in untreated control to 40% in H2O2-treated cells and an increase of cells in G2/M phase, from 12.62% in untreated control to 47% in injured cells (Figure 3B).


Evaluation of antioxidant and cytoprotective activities of Arnica montana L. and Artemisia absinthium L. ethanolic extracts.

Craciunescu O, Constantin D, Gaspar A, Toma L, Utoiu E, Moldovan L - Chem Cent J (2012)

A Effect of plant extracts on cell cycle distribution of NCTC cells. Co-treated and pre-treated cells were stained with propidium iodide and analyzed by flow-cytometry. B DNA histograms analyzed by MODFITTM LT 3.0 software. Results are represented as mean ± SD (n = 6). Each phase of individual samples was compared with correspondent phase in untreated control using paired t-test. Statistically significant differences were observed (##p < 0.01).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3472325&req=5

Figure 3: A Effect of plant extracts on cell cycle distribution of NCTC cells. Co-treated and pre-treated cells were stained with propidium iodide and analyzed by flow-cytometry. B DNA histograms analyzed by MODFITTM LT 3.0 software. Results are represented as mean ± SD (n = 6). Each phase of individual samples was compared with correspondent phase in untreated control using paired t-test. Statistically significant differences were observed (##p < 0.01).
Mentions: Cell cycle distribution was analyzed after treatment of H2O2-injured cells with 10 mg/L arnica and 300 mg/L wormwood, respectively. As shown in Figure 3A, H2O2-treated cells had an altered cycle that was arrested in G0/G1 and G2/M phases. The results showed a proportion of cells in G0/G1 phase that decreased from 60.61% in untreated control to 40% in H2O2-treated cells and an increase of cells in G2/M phase, from 12.62% in untreated control to 47% in injured cells (Figure 3B).

Bottom Line: Both plant extracts had significant effects on the growth of NCTC cells in the range of 10-100 mg/L A. montana and 10-500 mg/L A. absinthium.A. montana and A. absinthium extracts, rich in flavonoids and phenolic acids, showed a good antioxidant activity and cytoprotective effect against oxidative damage in fibroblast-like cells.These results provide scientific support for the traditional use of A. montana and A. absinthium in treatment of skin disorders.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Cellular and Molecular Biology, National Institute of Research and Development for Biological Sciences, 296, Splaiul Independentei, 060031, Bucharest, Romania. moldovanlc@yahoo.com.

ABSTRACT

Background: Arnica montana L. and Artemisia absinthium L. (Asteraceae) are medicinal plants native to temperate regions of Europe, including Romania, traditionally used for treatment of skin wounds, bruises and contusions. In the present study, A. montana and A. absinthium ethanolic extracts were evaluated for their chemical composition, antioxidant activity and protective effect against H2O2-induced oxidative stress in a mouse fibroblast-like NCTC cell line.

Results: A. absinthium extract showed a higher antioxidant capacity than A. montana extract as Trolox equivalent antioxidant capacity, Oxygen radical absorbance capacity and 2,2-diphenyl-1-picrylhydrazyl free radical-scavenging activity, in correlation with its flavonoids and phenolic acids content. Both plant extracts had significant effects on the growth of NCTC cells in the range of 10-100 mg/L A. montana and 10-500 mg/L A. absinthium. They also protected fibroblast cells against hydrogen peroxide-induced oxidative damage, at the same doses. The best protection was observed in cell pre-treatment with 10 mg/L A. montana and 10-300 mg/L A. absinthium, respectively, as determined by Neutral red and lactate dehydrogenase assays. In addition, cell pre-treatment with plant extracts, at these concentrations, prevented morphological changes induced by hydrogen peroxide. Flow-cytometry analysis showed that pre-treatment with A. montana and A. absinthium extracts restored the proportion of cells in each phase of the cell cycle.

Conclusions: A. montana and A. absinthium extracts, rich in flavonoids and phenolic acids, showed a good antioxidant activity and cytoprotective effect against oxidative damage in fibroblast-like cells. These results provide scientific support for the traditional use of A. montana and A. absinthium in treatment of skin disorders.

No MeSH data available.


Related in: MedlinePlus