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Evaluation of antioxidant and cytoprotective activities of Arnica montana L. and Artemisia absinthium L. ethanolic extracts.

Craciunescu O, Constantin D, Gaspar A, Toma L, Utoiu E, Moldovan L - Chem Cent J (2012)

Bottom Line: Both plant extracts had significant effects on the growth of NCTC cells in the range of 10-100 mg/L A. montana and 10-500 mg/L A. absinthium.A. montana and A. absinthium extracts, rich in flavonoids and phenolic acids, showed a good antioxidant activity and cytoprotective effect against oxidative damage in fibroblast-like cells.These results provide scientific support for the traditional use of A. montana and A. absinthium in treatment of skin disorders.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Cellular and Molecular Biology, National Institute of Research and Development for Biological Sciences, 296, Splaiul Independentei, 060031, Bucharest, Romania. moldovanlc@yahoo.com.

ABSTRACT

Background: Arnica montana L. and Artemisia absinthium L. (Asteraceae) are medicinal plants native to temperate regions of Europe, including Romania, traditionally used for treatment of skin wounds, bruises and contusions. In the present study, A. montana and A. absinthium ethanolic extracts were evaluated for their chemical composition, antioxidant activity and protective effect against H2O2-induced oxidative stress in a mouse fibroblast-like NCTC cell line.

Results: A. absinthium extract showed a higher antioxidant capacity than A. montana extract as Trolox equivalent antioxidant capacity, Oxygen radical absorbance capacity and 2,2-diphenyl-1-picrylhydrazyl free radical-scavenging activity, in correlation with its flavonoids and phenolic acids content. Both plant extracts had significant effects on the growth of NCTC cells in the range of 10-100 mg/L A. montana and 10-500 mg/L A. absinthium. They also protected fibroblast cells against hydrogen peroxide-induced oxidative damage, at the same doses. The best protection was observed in cell pre-treatment with 10 mg/L A. montana and 10-300 mg/L A. absinthium, respectively, as determined by Neutral red and lactate dehydrogenase assays. In addition, cell pre-treatment with plant extracts, at these concentrations, prevented morphological changes induced by hydrogen peroxide. Flow-cytometry analysis showed that pre-treatment with A. montana and A. absinthium extracts restored the proportion of cells in each phase of the cell cycle.

Conclusions: A. montana and A. absinthium extracts, rich in flavonoids and phenolic acids, showed a good antioxidant activity and cytoprotective effect against oxidative damage in fibroblast-like cells. These results provide scientific support for the traditional use of A. montana and A. absinthium in treatment of skin disorders.

No MeSH data available.


Related in: MedlinePlus

Viability of NCTC cells after co-treatment (A, C) and pre-treatment (B, D) with plant extracts at various concentrations, analyzed by Neutral red (A, B) and LDH (C, D) assays. Results are represented as mean ± SD (n = 6). ##p < 0.01 compared with untreated control (dotted); *p < 0.05 and **p < 0.01 compared with H2O2-treated group (striped). Micrographs taken at 24 h after H2O2-treatment showed the morphology of cells after co-treatment (E) and pre-treatment (F) with 10 mg/L A. montana extract and 300 mg/L A. absinthium extract. Scale bar = 10 μm.
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Figure 2: Viability of NCTC cells after co-treatment (A, C) and pre-treatment (B, D) with plant extracts at various concentrations, analyzed by Neutral red (A, B) and LDH (C, D) assays. Results are represented as mean ± SD (n = 6). ##p < 0.01 compared with untreated control (dotted); *p < 0.05 and **p < 0.01 compared with H2O2-treated group (striped). Micrographs taken at 24 h after H2O2-treatment showed the morphology of cells after co-treatment (E) and pre-treatment (F) with 10 mg/L A. montana extract and 300 mg/L A. absinthium extract. Scale bar = 10 μm.

Mentions: In order to evaluate the ability of both plant extracts to reduce oxidative stress in H2O2-treated cells, co-treatment and pre-treatment experiments were done and results are presented in Figure 2. Cell viability, membrane integrity, morphology and cell cycle were investigated. Co-treatment with 10–100 mg/L arnica extract or 100–500 mg/L wormwood extract, simultaneously with H2O2, presented viability values similar to H2O2-treated group (Figure 2A). Only the cells treated with 10 mg/L wormwood presented a significantly (p < 0.05) higher value of cell viability (approx. 60%) compared to H2O2-treated group (Figure 2A). LDH release readings for arnica and wormwood extract co-treatment groups did not vary significantly from H2O2-treated group (p > 0.05), with the exception of the 10 mg/L wormwood group that was significantly lower (p < 0.05) (Figure 2C). In comparison, pre-treatment of NCTC cells with arnica and wormwood extract, at each tested concentration, significantly reversed the H2O2-induced cytotoxicity. Neutral red test results presented values between 75–88.9% cell viability, significantly (p < 0.01) higher than H2O2-treated group (Figure 2B). The best cell protection, expressed as cell viability values, was observed for pre-treatment with 10 mg/L arnica and 10–300 mg/L wormwood, respectively. LDH secretion in the culture medium was significantly (p < 0.01) reduced when NCTC cells were pretreated with each concentration of arnica and wormwood extracts, compared to H2O2-treated group (Figure 2D).


Evaluation of antioxidant and cytoprotective activities of Arnica montana L. and Artemisia absinthium L. ethanolic extracts.

Craciunescu O, Constantin D, Gaspar A, Toma L, Utoiu E, Moldovan L - Chem Cent J (2012)

Viability of NCTC cells after co-treatment (A, C) and pre-treatment (B, D) with plant extracts at various concentrations, analyzed by Neutral red (A, B) and LDH (C, D) assays. Results are represented as mean ± SD (n = 6). ##p < 0.01 compared with untreated control (dotted); *p < 0.05 and **p < 0.01 compared with H2O2-treated group (striped). Micrographs taken at 24 h after H2O2-treatment showed the morphology of cells after co-treatment (E) and pre-treatment (F) with 10 mg/L A. montana extract and 300 mg/L A. absinthium extract. Scale bar = 10 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3472325&req=5

Figure 2: Viability of NCTC cells after co-treatment (A, C) and pre-treatment (B, D) with plant extracts at various concentrations, analyzed by Neutral red (A, B) and LDH (C, D) assays. Results are represented as mean ± SD (n = 6). ##p < 0.01 compared with untreated control (dotted); *p < 0.05 and **p < 0.01 compared with H2O2-treated group (striped). Micrographs taken at 24 h after H2O2-treatment showed the morphology of cells after co-treatment (E) and pre-treatment (F) with 10 mg/L A. montana extract and 300 mg/L A. absinthium extract. Scale bar = 10 μm.
Mentions: In order to evaluate the ability of both plant extracts to reduce oxidative stress in H2O2-treated cells, co-treatment and pre-treatment experiments were done and results are presented in Figure 2. Cell viability, membrane integrity, morphology and cell cycle were investigated. Co-treatment with 10–100 mg/L arnica extract or 100–500 mg/L wormwood extract, simultaneously with H2O2, presented viability values similar to H2O2-treated group (Figure 2A). Only the cells treated with 10 mg/L wormwood presented a significantly (p < 0.05) higher value of cell viability (approx. 60%) compared to H2O2-treated group (Figure 2A). LDH release readings for arnica and wormwood extract co-treatment groups did not vary significantly from H2O2-treated group (p > 0.05), with the exception of the 10 mg/L wormwood group that was significantly lower (p < 0.05) (Figure 2C). In comparison, pre-treatment of NCTC cells with arnica and wormwood extract, at each tested concentration, significantly reversed the H2O2-induced cytotoxicity. Neutral red test results presented values between 75–88.9% cell viability, significantly (p < 0.01) higher than H2O2-treated group (Figure 2B). The best cell protection, expressed as cell viability values, was observed for pre-treatment with 10 mg/L arnica and 10–300 mg/L wormwood, respectively. LDH secretion in the culture medium was significantly (p < 0.01) reduced when NCTC cells were pretreated with each concentration of arnica and wormwood extracts, compared to H2O2-treated group (Figure 2D).

Bottom Line: Both plant extracts had significant effects on the growth of NCTC cells in the range of 10-100 mg/L A. montana and 10-500 mg/L A. absinthium.A. montana and A. absinthium extracts, rich in flavonoids and phenolic acids, showed a good antioxidant activity and cytoprotective effect against oxidative damage in fibroblast-like cells.These results provide scientific support for the traditional use of A. montana and A. absinthium in treatment of skin disorders.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Cellular and Molecular Biology, National Institute of Research and Development for Biological Sciences, 296, Splaiul Independentei, 060031, Bucharest, Romania. moldovanlc@yahoo.com.

ABSTRACT

Background: Arnica montana L. and Artemisia absinthium L. (Asteraceae) are medicinal plants native to temperate regions of Europe, including Romania, traditionally used for treatment of skin wounds, bruises and contusions. In the present study, A. montana and A. absinthium ethanolic extracts were evaluated for their chemical composition, antioxidant activity and protective effect against H2O2-induced oxidative stress in a mouse fibroblast-like NCTC cell line.

Results: A. absinthium extract showed a higher antioxidant capacity than A. montana extract as Trolox equivalent antioxidant capacity, Oxygen radical absorbance capacity and 2,2-diphenyl-1-picrylhydrazyl free radical-scavenging activity, in correlation with its flavonoids and phenolic acids content. Both plant extracts had significant effects on the growth of NCTC cells in the range of 10-100 mg/L A. montana and 10-500 mg/L A. absinthium. They also protected fibroblast cells against hydrogen peroxide-induced oxidative damage, at the same doses. The best protection was observed in cell pre-treatment with 10 mg/L A. montana and 10-300 mg/L A. absinthium, respectively, as determined by Neutral red and lactate dehydrogenase assays. In addition, cell pre-treatment with plant extracts, at these concentrations, prevented morphological changes induced by hydrogen peroxide. Flow-cytometry analysis showed that pre-treatment with A. montana and A. absinthium extracts restored the proportion of cells in each phase of the cell cycle.

Conclusions: A. montana and A. absinthium extracts, rich in flavonoids and phenolic acids, showed a good antioxidant activity and cytoprotective effect against oxidative damage in fibroblast-like cells. These results provide scientific support for the traditional use of A. montana and A. absinthium in treatment of skin disorders.

No MeSH data available.


Related in: MedlinePlus