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Evaluation of antioxidant and cytoprotective activities of Arnica montana L. and Artemisia absinthium L. ethanolic extracts.

Craciunescu O, Constantin D, Gaspar A, Toma L, Utoiu E, Moldovan L - Chem Cent J (2012)

Bottom Line: Both plant extracts had significant effects on the growth of NCTC cells in the range of 10-100 mg/L A. montana and 10-500 mg/L A. absinthium.A. montana and A. absinthium extracts, rich in flavonoids and phenolic acids, showed a good antioxidant activity and cytoprotective effect against oxidative damage in fibroblast-like cells.These results provide scientific support for the traditional use of A. montana and A. absinthium in treatment of skin disorders.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Cellular and Molecular Biology, National Institute of Research and Development for Biological Sciences, 296, Splaiul Independentei, 060031, Bucharest, Romania. moldovanlc@yahoo.com.

ABSTRACT

Background: Arnica montana L. and Artemisia absinthium L. (Asteraceae) are medicinal plants native to temperate regions of Europe, including Romania, traditionally used for treatment of skin wounds, bruises and contusions. In the present study, A. montana and A. absinthium ethanolic extracts were evaluated for their chemical composition, antioxidant activity and protective effect against H2O2-induced oxidative stress in a mouse fibroblast-like NCTC cell line.

Results: A. absinthium extract showed a higher antioxidant capacity than A. montana extract as Trolox equivalent antioxidant capacity, Oxygen radical absorbance capacity and 2,2-diphenyl-1-picrylhydrazyl free radical-scavenging activity, in correlation with its flavonoids and phenolic acids content. Both plant extracts had significant effects on the growth of NCTC cells in the range of 10-100 mg/L A. montana and 10-500 mg/L A. absinthium. They also protected fibroblast cells against hydrogen peroxide-induced oxidative damage, at the same doses. The best protection was observed in cell pre-treatment with 10 mg/L A. montana and 10-300 mg/L A. absinthium, respectively, as determined by Neutral red and lactate dehydrogenase assays. In addition, cell pre-treatment with plant extracts, at these concentrations, prevented morphological changes induced by hydrogen peroxide. Flow-cytometry analysis showed that pre-treatment with A. montana and A. absinthium extracts restored the proportion of cells in each phase of the cell cycle.

Conclusions: A. montana and A. absinthium extracts, rich in flavonoids and phenolic acids, showed a good antioxidant activity and cytoprotective effect against oxidative damage in fibroblast-like cells. These results provide scientific support for the traditional use of A. montana and A. absinthium in treatment of skin disorders.

No MeSH data available.


Related in: MedlinePlus

Cell viability of NCTC cells cultured with different concentrations of  A. montana L. and  A. absinthium L. extracts, analyzed by Neutral red (A) and LDH (B) assays. The negative control was represented by cells cultivated in culture plate, in MEM (dotted) and the positive control was represented by cells cultivated in MEM containing 100 μM H2O2 (striped). Results are shown as mean ± SD (n = 6). Pairs of negative control and each sample were analyzed by t-test. Significant differences in each pair are marked with #p < 0.05 or ##p < 0.01.
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Figure 1: Cell viability of NCTC cells cultured with different concentrations of A. montana L. and A. absinthium L. extracts, analyzed by Neutral red (A) and LDH (B) assays. The negative control was represented by cells cultivated in culture plate, in MEM (dotted) and the positive control was represented by cells cultivated in MEM containing 100 μM H2O2 (striped). Results are shown as mean ± SD (n = 6). Pairs of negative control and each sample were analyzed by t-test. Significant differences in each pair are marked with #p < 0.05 or ##p < 0.01.

Mentions: To exclude the possible cytotoxic effect of of arnica and wormwood extracts, several concentrations (10–1000 mg/L) were tested on NCTC cells. As shown in Figure 1, cell treatment with each concentration of plant extract, for 24 h, induced a dose-dependent effect on cell viability values. Higher values of cell viability (above 80%) were recorded for concentrations up to 100 mg/L of arnica extract and up to 500 mg/L of wormwood extract (Figure 1A). Higher concentrations induced disturbance of Neutral red retention and cell viability significantly decreased (p < 0.01) down to 30.46%, compared with negative control (100%) (Figure 1A).


Evaluation of antioxidant and cytoprotective activities of Arnica montana L. and Artemisia absinthium L. ethanolic extracts.

Craciunescu O, Constantin D, Gaspar A, Toma L, Utoiu E, Moldovan L - Chem Cent J (2012)

Cell viability of NCTC cells cultured with different concentrations of  A. montana L. and  A. absinthium L. extracts, analyzed by Neutral red (A) and LDH (B) assays. The negative control was represented by cells cultivated in culture plate, in MEM (dotted) and the positive control was represented by cells cultivated in MEM containing 100 μM H2O2 (striped). Results are shown as mean ± SD (n = 6). Pairs of negative control and each sample were analyzed by t-test. Significant differences in each pair are marked with #p < 0.05 or ##p < 0.01.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3472325&req=5

Figure 1: Cell viability of NCTC cells cultured with different concentrations of A. montana L. and A. absinthium L. extracts, analyzed by Neutral red (A) and LDH (B) assays. The negative control was represented by cells cultivated in culture plate, in MEM (dotted) and the positive control was represented by cells cultivated in MEM containing 100 μM H2O2 (striped). Results are shown as mean ± SD (n = 6). Pairs of negative control and each sample were analyzed by t-test. Significant differences in each pair are marked with #p < 0.05 or ##p < 0.01.
Mentions: To exclude the possible cytotoxic effect of of arnica and wormwood extracts, several concentrations (10–1000 mg/L) were tested on NCTC cells. As shown in Figure 1, cell treatment with each concentration of plant extract, for 24 h, induced a dose-dependent effect on cell viability values. Higher values of cell viability (above 80%) were recorded for concentrations up to 100 mg/L of arnica extract and up to 500 mg/L of wormwood extract (Figure 1A). Higher concentrations induced disturbance of Neutral red retention and cell viability significantly decreased (p < 0.01) down to 30.46%, compared with negative control (100%) (Figure 1A).

Bottom Line: Both plant extracts had significant effects on the growth of NCTC cells in the range of 10-100 mg/L A. montana and 10-500 mg/L A. absinthium.A. montana and A. absinthium extracts, rich in flavonoids and phenolic acids, showed a good antioxidant activity and cytoprotective effect against oxidative damage in fibroblast-like cells.These results provide scientific support for the traditional use of A. montana and A. absinthium in treatment of skin disorders.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Cellular and Molecular Biology, National Institute of Research and Development for Biological Sciences, 296, Splaiul Independentei, 060031, Bucharest, Romania. moldovanlc@yahoo.com.

ABSTRACT

Background: Arnica montana L. and Artemisia absinthium L. (Asteraceae) are medicinal plants native to temperate regions of Europe, including Romania, traditionally used for treatment of skin wounds, bruises and contusions. In the present study, A. montana and A. absinthium ethanolic extracts were evaluated for their chemical composition, antioxidant activity and protective effect against H2O2-induced oxidative stress in a mouse fibroblast-like NCTC cell line.

Results: A. absinthium extract showed a higher antioxidant capacity than A. montana extract as Trolox equivalent antioxidant capacity, Oxygen radical absorbance capacity and 2,2-diphenyl-1-picrylhydrazyl free radical-scavenging activity, in correlation with its flavonoids and phenolic acids content. Both plant extracts had significant effects on the growth of NCTC cells in the range of 10-100 mg/L A. montana and 10-500 mg/L A. absinthium. They also protected fibroblast cells against hydrogen peroxide-induced oxidative damage, at the same doses. The best protection was observed in cell pre-treatment with 10 mg/L A. montana and 10-300 mg/L A. absinthium, respectively, as determined by Neutral red and lactate dehydrogenase assays. In addition, cell pre-treatment with plant extracts, at these concentrations, prevented morphological changes induced by hydrogen peroxide. Flow-cytometry analysis showed that pre-treatment with A. montana and A. absinthium extracts restored the proportion of cells in each phase of the cell cycle.

Conclusions: A. montana and A. absinthium extracts, rich in flavonoids and phenolic acids, showed a good antioxidant activity and cytoprotective effect against oxidative damage in fibroblast-like cells. These results provide scientific support for the traditional use of A. montana and A. absinthium in treatment of skin disorders.

No MeSH data available.


Related in: MedlinePlus