Limits...
Differential gene expression profiling of porcine epithelial cells infected with three enterotoxigenic Escherichia coli strains.

Zhou C, Liu Z, Jiang J, Yu Y, Zhang Q - BMC Genomics (2012)

Bottom Line: In ETEC strains, the fimbrial types F4 and F18 are commonly found differently colonized within the small intestine and cause huge economic losses in the swine industry annually worldwide.The gene ontology and pathway analysis showed that the differentially expressed genes in C(F4ab)vs control are significantly involved in cell-cycle progress and amino acid metabolism, while the clustered terms of the differentially expressed genes in C(F4ac)vs control comprise immune, inflammation and wounding response and apoptosis as well as cell cycle progress and proteolysis.The genes induced by ETECs with F4 versus F18 fimbriae suggest why ETEC with F4 may be more virulent compared to F18 which seems to elicit milder effects.

View Article: PubMed Central - HTML - PubMed

Affiliation: Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, 100193 Beijing, Peoples Republic of China.

ABSTRACT

Background: Enterotoxigenic Escherichia coli (ETEC) is one of the most important pathogenic bacteria causing severe diarrhoea in human and pigs. In ETEC strains, the fimbrial types F4 and F18 are commonly found differently colonized within the small intestine and cause huge economic losses in the swine industry annually worldwide. To address the underlying mechanism, we performed a transcriptome study of porcine intestinal epithelial cells (IPEC-J2) with and without infection of three representative ETEC strains.

Results: A total 2443, 3493 and 867 differentially expressed genes were found in IPEC-J2 cells infected with F4ab ETEC (C(F4ab)), with F4ac ETEC (C(F4ac)) and with F18ac ETEC (C(F18ac)) compared to the cells without infection (control), respectively. The number of differentially expressed genes between C(F4ab) and C(F4ac), C(F4ab) and C(F18ac), and C(F4ac) and C(F18ac) were 77, 1446 and 1629, respectively. The gene ontology and pathway analysis showed that the differentially expressed genes in C(F4ab)vs control are significantly involved in cell-cycle progress and amino acid metabolism, while the clustered terms of the differentially expressed genes in C(F4ac)vs control comprise immune, inflammation and wounding response and apoptosis as well as cell cycle progress and proteolysis. Differentially expressed genes between C(F18ac)vs control are mainly involved in cell-cycle progression and immune response. Furthermore, fundamental differences were observed in expression levels of immune-related genes among the three ETEC treatments, especially for the important pro-inflammatory molecules, including IL-6, IL-8, TNF-α, CCL20, CXCL2 etc.

Conclusions: The discovery in this study provides insights into the interaction of porcine intestinal epithelial cells with F4 ETECs and F18 ETEC, respectively. The genes induced by ETECs with F4 versus F18 fimbriae suggest why ETEC with F4 may be more virulent compared to F18 which seems to elicit milder effects.

Show MeSH

Related in: MedlinePlus

Immune response of IPEC-J2 cells 3 h after infection. A graphical representation of a robust response to infection was adapted from [40,41]. Genes that were differentially expressed in the IPEC-J2 cells upon different ETEC strains infection are illustrated. Further differentiating was that genes responded to the F4ab ETEC infection only are in blue colour, to F4ac ETEC only are in black, to F18ac ETEC only are in purple, to both F4ac ETEC and F18ac ETEC are in black with underline, to both F4ab ETEC and F4ac ETEC are in red without underline, and to all of the three strains are in red with underline. Genes marked with a downward arrow were down-regulated and the others up-regulated.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3472312&req=5

Figure 3: Immune response of IPEC-J2 cells 3 h after infection. A graphical representation of a robust response to infection was adapted from [40,41]. Genes that were differentially expressed in the IPEC-J2 cells upon different ETEC strains infection are illustrated. Further differentiating was that genes responded to the F4ab ETEC infection only are in blue colour, to F4ac ETEC only are in black, to F18ac ETEC only are in purple, to both F4ac ETEC and F18ac ETEC are in black with underline, to both F4ab ETEC and F4ac ETEC are in red without underline, and to all of the three strains are in red with underline. Genes marked with a downward arrow were down-regulated and the others up-regulated.

Mentions: Intestinal epithelial cells (IEC) are pivotal for the activation of innate immunity and subsequently for the induction of adaptive immune responses [4,39]. We found numerous important immune-related genes were differentially expressed upon separate infection with each of the three ETEC strains (Additional file 3). Similar to that described in the reports of Mitterhuemer et al. and Jenner et al.[40,41], we could also integrate our findings into a scheme to describe the transcriptional response of IPEC-J2 to ETECs infections (Figure 3), which clearly interprets the pathogen-host interaction of ETECs and IPEC-J2 cells after 3 h co-culture. Consistent with earlier findings, we observed F4 ETECs (F4ab ETEC and F4ac ETEC) could significantly enhance the expression of IL-6 and IL-8 cytokine [4,42], while F18ac ETEC could only enhance the expression of IL-6, which confirmed the principal idea that apical membrane of the intestinal epithelial cells represent a mechanical barrier against pathogens firstly [43].


Differential gene expression profiling of porcine epithelial cells infected with three enterotoxigenic Escherichia coli strains.

Zhou C, Liu Z, Jiang J, Yu Y, Zhang Q - BMC Genomics (2012)

Immune response of IPEC-J2 cells 3 h after infection. A graphical representation of a robust response to infection was adapted from [40,41]. Genes that were differentially expressed in the IPEC-J2 cells upon different ETEC strains infection are illustrated. Further differentiating was that genes responded to the F4ab ETEC infection only are in blue colour, to F4ac ETEC only are in black, to F18ac ETEC only are in purple, to both F4ac ETEC and F18ac ETEC are in black with underline, to both F4ab ETEC and F4ac ETEC are in red without underline, and to all of the three strains are in red with underline. Genes marked with a downward arrow were down-regulated and the others up-regulated.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3472312&req=5

Figure 3: Immune response of IPEC-J2 cells 3 h after infection. A graphical representation of a robust response to infection was adapted from [40,41]. Genes that were differentially expressed in the IPEC-J2 cells upon different ETEC strains infection are illustrated. Further differentiating was that genes responded to the F4ab ETEC infection only are in blue colour, to F4ac ETEC only are in black, to F18ac ETEC only are in purple, to both F4ac ETEC and F18ac ETEC are in black with underline, to both F4ab ETEC and F4ac ETEC are in red without underline, and to all of the three strains are in red with underline. Genes marked with a downward arrow were down-regulated and the others up-regulated.
Mentions: Intestinal epithelial cells (IEC) are pivotal for the activation of innate immunity and subsequently for the induction of adaptive immune responses [4,39]. We found numerous important immune-related genes were differentially expressed upon separate infection with each of the three ETEC strains (Additional file 3). Similar to that described in the reports of Mitterhuemer et al. and Jenner et al.[40,41], we could also integrate our findings into a scheme to describe the transcriptional response of IPEC-J2 to ETECs infections (Figure 3), which clearly interprets the pathogen-host interaction of ETECs and IPEC-J2 cells after 3 h co-culture. Consistent with earlier findings, we observed F4 ETECs (F4ab ETEC and F4ac ETEC) could significantly enhance the expression of IL-6 and IL-8 cytokine [4,42], while F18ac ETEC could only enhance the expression of IL-6, which confirmed the principal idea that apical membrane of the intestinal epithelial cells represent a mechanical barrier against pathogens firstly [43].

Bottom Line: In ETEC strains, the fimbrial types F4 and F18 are commonly found differently colonized within the small intestine and cause huge economic losses in the swine industry annually worldwide.The gene ontology and pathway analysis showed that the differentially expressed genes in C(F4ab)vs control are significantly involved in cell-cycle progress and amino acid metabolism, while the clustered terms of the differentially expressed genes in C(F4ac)vs control comprise immune, inflammation and wounding response and apoptosis as well as cell cycle progress and proteolysis.The genes induced by ETECs with F4 versus F18 fimbriae suggest why ETEC with F4 may be more virulent compared to F18 which seems to elicit milder effects.

View Article: PubMed Central - HTML - PubMed

Affiliation: Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, 100193 Beijing, Peoples Republic of China.

ABSTRACT

Background: Enterotoxigenic Escherichia coli (ETEC) is one of the most important pathogenic bacteria causing severe diarrhoea in human and pigs. In ETEC strains, the fimbrial types F4 and F18 are commonly found differently colonized within the small intestine and cause huge economic losses in the swine industry annually worldwide. To address the underlying mechanism, we performed a transcriptome study of porcine intestinal epithelial cells (IPEC-J2) with and without infection of three representative ETEC strains.

Results: A total 2443, 3493 and 867 differentially expressed genes were found in IPEC-J2 cells infected with F4ab ETEC (C(F4ab)), with F4ac ETEC (C(F4ac)) and with F18ac ETEC (C(F18ac)) compared to the cells without infection (control), respectively. The number of differentially expressed genes between C(F4ab) and C(F4ac), C(F4ab) and C(F18ac), and C(F4ac) and C(F18ac) were 77, 1446 and 1629, respectively. The gene ontology and pathway analysis showed that the differentially expressed genes in C(F4ab)vs control are significantly involved in cell-cycle progress and amino acid metabolism, while the clustered terms of the differentially expressed genes in C(F4ac)vs control comprise immune, inflammation and wounding response and apoptosis as well as cell cycle progress and proteolysis. Differentially expressed genes between C(F18ac)vs control are mainly involved in cell-cycle progression and immune response. Furthermore, fundamental differences were observed in expression levels of immune-related genes among the three ETEC treatments, especially for the important pro-inflammatory molecules, including IL-6, IL-8, TNF-α, CCL20, CXCL2 etc.

Conclusions: The discovery in this study provides insights into the interaction of porcine intestinal epithelial cells with F4 ETECs and F18 ETEC, respectively. The genes induced by ETECs with F4 versus F18 fimbriae suggest why ETEC with F4 may be more virulent compared to F18 which seems to elicit milder effects.

Show MeSH
Related in: MedlinePlus