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The venom-gland transcriptome of the eastern diamondback rattlesnake (Crotalus adamanteus).

Rokyta DR, Lemmon AR, Margres MJ, Aronow K - BMC Genomics (2012)

Bottom Line: The most diverse toxin classes were the C-type lectins (21 clusters), the snake-venom metalloproteinases (16 clusters), and the serine proteinases (14 clusters).The high-abundance nontoxin transcripts were predominantly those involved in protein folding and translation, consistent with the protein-secretory function of the tissue.We have more than doubled the number of sequenced toxins for this species and created extensive genomic resources for snakes based entirely on de novo assembly of Illumina sequence data.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biological Science, Florida State University, Tallahassee, FL 32306-4295, USA. drokyta@bio.fsu.edu

ABSTRACT

Background: Snake venoms have significant impacts on human populations through the morbidity and mortality associated with snakebites and as sources of drugs, drug leads, and physiological research tools. Genes expressed by venom-gland tissue, including those encoding toxic proteins, have therefore been sequenced but only with relatively sparse coverage resulting from the low-throughput sequencing approaches available. High-throughput approaches based on 454 pyrosequencing have recently been applied to the study of snake venoms to give the most complete characterizations to date of the genes expressed in active venom glands, but such approaches are costly and still provide a far-from-complete characterization of the genes expressed during venom production.

Results: We describe the de novo assembly and analysis of the venom-gland transcriptome of an eastern diamondback rattlesnake (Crotalus adamanteus) based on 95,643,958 pairs of quality-filtered, 100-base-pair Illumina reads. We identified 123 unique, full-length toxin-coding sequences, which cluster into 78 groups with less than 1% nucleotide divergence, and 2,879 unique, full-length nontoxin coding sequences. The toxin sequences accounted for 35.4% of the total reads, and the nontoxin sequences for an additional 27.5%. The most highly expressed toxin was a small myotoxin related to crotamine, which accounted for 5.9% of the total reads. Snake-venom metalloproteinases accounted for the highest percentage of reads mapping to a toxin class (24.4%), followed by C-type lectins (22.2%) and serine proteinases (20.0%). The most diverse toxin classes were the C-type lectins (21 clusters), the snake-venom metalloproteinases (16 clusters), and the serine proteinases (14 clusters). The high-abundance nontoxin transcripts were predominantly those involved in protein folding and translation, consistent with the protein-secretory function of the tissue.

Conclusions: We have provided the most complete characterization of the genes expressed in an active snake venom gland to date, producing insights into snakebite pathology and guidance for snakebite treatment for the largest rattlesnake species and arguably the most dangerous snake native to the United States of America, C. adamanteus. We have more than doubled the number of sequenced toxins for this species and created extensive genomic resources for snakes based entirely on de novo assembly of Illumina sequence data.

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Expression levels of major classes of toxins and nontoxins. More than 60% of the total reads have been accounted for with full-length annotated transcripts. (A) The major toxin classes were the CTLs, SVSPs, MYO, and SVMPs (types II and III). (B) As expected for a protein-secreting tissue, the venom gland expresses an abundance of proteins involved in proteostasis.
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Figure 3: Expression levels of major classes of toxins and nontoxins. More than 60% of the total reads have been accounted for with full-length annotated transcripts. (A) The major toxin classes were the CTLs, SVSPs, MYO, and SVMPs (types II and III). (B) As expected for a protein-secreting tissue, the venom gland expresses an abundance of proteins involved in proteostasis.

Mentions: The results from both assembly approaches were merged to yield the final data set. The first approach produced 72 unique toxin and 234 unique nontoxin sequences, and the second 91 toxin and 2,851 nontoxin sequences. The merged data set consisted of 123 unique toxin sequences and 2,879 nontoxins that together accounted for 62.9% of the sequencing reads (Figure 3).


The venom-gland transcriptome of the eastern diamondback rattlesnake (Crotalus adamanteus).

Rokyta DR, Lemmon AR, Margres MJ, Aronow K - BMC Genomics (2012)

Expression levels of major classes of toxins and nontoxins. More than 60% of the total reads have been accounted for with full-length annotated transcripts. (A) The major toxin classes were the CTLs, SVSPs, MYO, and SVMPs (types II and III). (B) As expected for a protein-secreting tissue, the venom gland expresses an abundance of proteins involved in proteostasis.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3472243&req=5

Figure 3: Expression levels of major classes of toxins and nontoxins. More than 60% of the total reads have been accounted for with full-length annotated transcripts. (A) The major toxin classes were the CTLs, SVSPs, MYO, and SVMPs (types II and III). (B) As expected for a protein-secreting tissue, the venom gland expresses an abundance of proteins involved in proteostasis.
Mentions: The results from both assembly approaches were merged to yield the final data set. The first approach produced 72 unique toxin and 234 unique nontoxin sequences, and the second 91 toxin and 2,851 nontoxin sequences. The merged data set consisted of 123 unique toxin sequences and 2,879 nontoxins that together accounted for 62.9% of the sequencing reads (Figure 3).

Bottom Line: The most diverse toxin classes were the C-type lectins (21 clusters), the snake-venom metalloproteinases (16 clusters), and the serine proteinases (14 clusters).The high-abundance nontoxin transcripts were predominantly those involved in protein folding and translation, consistent with the protein-secretory function of the tissue.We have more than doubled the number of sequenced toxins for this species and created extensive genomic resources for snakes based entirely on de novo assembly of Illumina sequence data.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biological Science, Florida State University, Tallahassee, FL 32306-4295, USA. drokyta@bio.fsu.edu

ABSTRACT

Background: Snake venoms have significant impacts on human populations through the morbidity and mortality associated with snakebites and as sources of drugs, drug leads, and physiological research tools. Genes expressed by venom-gland tissue, including those encoding toxic proteins, have therefore been sequenced but only with relatively sparse coverage resulting from the low-throughput sequencing approaches available. High-throughput approaches based on 454 pyrosequencing have recently been applied to the study of snake venoms to give the most complete characterizations to date of the genes expressed in active venom glands, but such approaches are costly and still provide a far-from-complete characterization of the genes expressed during venom production.

Results: We describe the de novo assembly and analysis of the venom-gland transcriptome of an eastern diamondback rattlesnake (Crotalus adamanteus) based on 95,643,958 pairs of quality-filtered, 100-base-pair Illumina reads. We identified 123 unique, full-length toxin-coding sequences, which cluster into 78 groups with less than 1% nucleotide divergence, and 2,879 unique, full-length nontoxin coding sequences. The toxin sequences accounted for 35.4% of the total reads, and the nontoxin sequences for an additional 27.5%. The most highly expressed toxin was a small myotoxin related to crotamine, which accounted for 5.9% of the total reads. Snake-venom metalloproteinases accounted for the highest percentage of reads mapping to a toxin class (24.4%), followed by C-type lectins (22.2%) and serine proteinases (20.0%). The most diverse toxin classes were the C-type lectins (21 clusters), the snake-venom metalloproteinases (16 clusters), and the serine proteinases (14 clusters). The high-abundance nontoxin transcripts were predominantly those involved in protein folding and translation, consistent with the protein-secretory function of the tissue.

Conclusions: We have provided the most complete characterization of the genes expressed in an active snake venom gland to date, producing insights into snakebite pathology and guidance for snakebite treatment for the largest rattlesnake species and arguably the most dangerous snake native to the United States of America, C. adamanteus. We have more than doubled the number of sequenced toxins for this species and created extensive genomic resources for snakes based entirely on de novo assembly of Illumina sequence data.

Show MeSH
Related in: MedlinePlus