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The extravascular compartment of the bone marrow: a niche for Plasmodium falciparum gametocyte maturation?

Farfour E, Charlotte F, Settegrana C, Miyara M, Buffet P - Malar. J. (2012)

Bottom Line: Plasmodium falciparum immature gametocytes accumulate in the bone marrow, but their exact location in this tissue remains unclear.A majority (89%) of immature stages II to IV gametocytes and a minority (29%) of mature stage V gametocytes were observed in extravascular spaces.These observations represent a valuable step towards understanding sequestration patterns of P. falciparum gametocytes and may ultimately lead to novel transmission-blocking interventions.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratoire de parasitologie-mycologie, Assistance publique hôpitaux de Paris, Groupe Hospitalier Pitié-Salpêtrière, 47 Boulevard de l'Hôpital, 75651 Paris Cedex 13, France.

ABSTRACT

Background: Plasmodium falciparum immature gametocytes accumulate in the bone marrow, but their exact location in this tissue remains unclear.

Methods: The stage and deposition pattern of gametocytes was analysed on histological sections of a bone marrow sample collected in a patient with subacute P. falciparum malaria.

Results: A majority (89%) of immature stages II to IV gametocytes and a minority (29%) of mature stage V gametocytes were observed in extravascular spaces.

Discussion and conclusion: These observations represent a valuable step towards understanding sequestration patterns of P. falciparum gametocytes and may ultimately lead to novel transmission-blocking interventions.

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Related in: MedlinePlus

A (arrow and Ai insert) and B: HES-stained bone marrow sections showing immature gametocytes - elongated shape, pink and purple staining, finely distributed pigment – outside small vessels (vl) and in close contact with resident cells such as adipocytes (ad) or erythroblasts (e). The dotted line in A highlights the vessel wall. C: Mature stage V gametocyte close to a mature red blood cell (C, r) in the lumen of a vessel. D and E: Immunohistochemistry (antibodies revealed with peroxydase) showing gametocytes (arrows) distant from CD34-positive vascular structures (vl) or CD68-positive macrophages (mf).
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Figure 2: A (arrow and Ai insert) and B: HES-stained bone marrow sections showing immature gametocytes - elongated shape, pink and purple staining, finely distributed pigment – outside small vessels (vl) and in close contact with resident cells such as adipocytes (ad) or erythroblasts (e). The dotted line in A highlights the vessel wall. C: Mature stage V gametocyte close to a mature red blood cell (C, r) in the lumen of a vessel. D and E: Immunohistochemistry (antibodies revealed with peroxydase) showing gametocytes (arrows) distant from CD34-positive vascular structures (vl) or CD68-positive macrophages (mf).

Mentions: Stained histological sections of the bone marrow showed gametocytes at different maturation stages and numerous macrophages containing blood cells or malaria pigment. Gametocytes were readily identified and staged based on their oval or elongated shape and finely distributed pigment (Figure 1). Only 29% (4/14) of stage V gametocytes were in extravascular spaces (Table 1), the majority being in intravascular spaces (Figure 2C). By contrast, 89% (87/98) of immature stage II-IV gametocytes were observed in extravascular spaces (Table 1, Figure 2) in immediate contact with erythroblasts (Figure 2B), adipocytes (Figure 2A) or other nucleated cells (Figure 2B). Several gametocytes did not co-localize with CD34-positive vascular structures (Figure 2D) or CD68-positive cells (Figure 2E).


The extravascular compartment of the bone marrow: a niche for Plasmodium falciparum gametocyte maturation?

Farfour E, Charlotte F, Settegrana C, Miyara M, Buffet P - Malar. J. (2012)

A (arrow and Ai insert) and B: HES-stained bone marrow sections showing immature gametocytes - elongated shape, pink and purple staining, finely distributed pigment – outside small vessels (vl) and in close contact with resident cells such as adipocytes (ad) or erythroblasts (e). The dotted line in A highlights the vessel wall. C: Mature stage V gametocyte close to a mature red blood cell (C, r) in the lumen of a vessel. D and E: Immunohistochemistry (antibodies revealed with peroxydase) showing gametocytes (arrows) distant from CD34-positive vascular structures (vl) or CD68-positive macrophages (mf).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3472181&req=5

Figure 2: A (arrow and Ai insert) and B: HES-stained bone marrow sections showing immature gametocytes - elongated shape, pink and purple staining, finely distributed pigment – outside small vessels (vl) and in close contact with resident cells such as adipocytes (ad) or erythroblasts (e). The dotted line in A highlights the vessel wall. C: Mature stage V gametocyte close to a mature red blood cell (C, r) in the lumen of a vessel. D and E: Immunohistochemistry (antibodies revealed with peroxydase) showing gametocytes (arrows) distant from CD34-positive vascular structures (vl) or CD68-positive macrophages (mf).
Mentions: Stained histological sections of the bone marrow showed gametocytes at different maturation stages and numerous macrophages containing blood cells or malaria pigment. Gametocytes were readily identified and staged based on their oval or elongated shape and finely distributed pigment (Figure 1). Only 29% (4/14) of stage V gametocytes were in extravascular spaces (Table 1), the majority being in intravascular spaces (Figure 2C). By contrast, 89% (87/98) of immature stage II-IV gametocytes were observed in extravascular spaces (Table 1, Figure 2) in immediate contact with erythroblasts (Figure 2B), adipocytes (Figure 2A) or other nucleated cells (Figure 2B). Several gametocytes did not co-localize with CD34-positive vascular structures (Figure 2D) or CD68-positive cells (Figure 2E).

Bottom Line: Plasmodium falciparum immature gametocytes accumulate in the bone marrow, but their exact location in this tissue remains unclear.A majority (89%) of immature stages II to IV gametocytes and a minority (29%) of mature stage V gametocytes were observed in extravascular spaces.These observations represent a valuable step towards understanding sequestration patterns of P. falciparum gametocytes and may ultimately lead to novel transmission-blocking interventions.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratoire de parasitologie-mycologie, Assistance publique hôpitaux de Paris, Groupe Hospitalier Pitié-Salpêtrière, 47 Boulevard de l'Hôpital, 75651 Paris Cedex 13, France.

ABSTRACT

Background: Plasmodium falciparum immature gametocytes accumulate in the bone marrow, but their exact location in this tissue remains unclear.

Methods: The stage and deposition pattern of gametocytes was analysed on histological sections of a bone marrow sample collected in a patient with subacute P. falciparum malaria.

Results: A majority (89%) of immature stages II to IV gametocytes and a minority (29%) of mature stage V gametocytes were observed in extravascular spaces.

Discussion and conclusion: These observations represent a valuable step towards understanding sequestration patterns of P. falciparum gametocytes and may ultimately lead to novel transmission-blocking interventions.

Show MeSH
Related in: MedlinePlus