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Expression of human Piwi-like genes is associated with prognosis for soft tissue sarcoma patients.

Greither T, Koser F, Kappler M, Bache M, Lautenschläger C, Göbel S, Holzhausen HJ, Wach S, Würl P, Taubert H - BMC Cancer (2012)

Bottom Line: In multivariate Cox's regression analyses, we showed that low Piwi-like 2 and Piwi-like 4 mRNA expression were significantly associated with a worse prognosis (RR = 1.87; p = 0.032 and RR = 1.82; p = 0.039).However, combined low Piwi-like 2 and 3 transcript levels were associated with worse survival (RR = 5.90; p = 0.02) for male patients.In this study, we identified a significant association between the expression of Piwi-like 2 and 4 mRNAs and the tumor-specific survival of soft tissue sarcoma patients.

View Article: PubMed Central - HTML - PubMed

Affiliation: Div, Molecular Urology, FAU Erlangen-Nürnberg, University Clinic of Urology, Erlangen, Germany.

ABSTRACT

Background: Argonaute genes are essential for RNA interference, stem cell maintenance and differentiation. The Piwi-like genes, a subclass of the Argonaute genes, are expressed mainly in the germline. These genes may be re-expressed in tumors, and expression of the Piwi-like genes is associated with prognosis in several types of tumors.

Methods: We measured the expression of Piwi-like mRNAs (Piwi-like 2-4) in 125 soft tissue sarcoma (STS) samples by qPCRs. Statistical tests were applied to study the correlation of expression levels with tumor-specific survival for STS patients.

Results: In multivariate Cox's regression analyses, we showed that low Piwi-like 2 and Piwi-like 4 mRNA expression were significantly associated with a worse prognosis (RR = 1.87; p = 0.032 and RR = 1.82; p = 0.039). Low expression of both genes was associated with a 2.58-fold increased risk of tumor-related death (p = 0.01). Piwi-like 4 and combined Piwi-like 2 and 4 mRNA levels correlated significantly with prognosis (RR = 3.53; p = 0.002 and RR = 5.23; p = 0.004) only for female but not for male patients. However, combined low Piwi-like 2 and 3 transcript levels were associated with worse survival (RR = 5.90; p = 0.02) for male patients.

Conclusions: In this study, we identified a significant association between the expression of Piwi-like 2 and 4 mRNAs and the tumor-specific survival of soft tissue sarcoma patients. Furthermore, a connection between sex and the impact of Piwi-like mRNA expressions on STS patients' prognosis was shown for the first time.

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Multivariate Cox’s regression analyses: A. Combined expression of Piwi-like 2 and 3 in male STS patients and B. combined Piwi-like 2 and −4 expression in female STS patients and their correlation with tumor-specific survival.
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Figure 1: Multivariate Cox’s regression analyses: A. Combined expression of Piwi-like 2 and 3 in male STS patients and B. combined Piwi-like 2 and −4 expression in female STS patients and their correlation with tumor-specific survival.

Mentions: Finally, we analyzed the combined expression of the Piwi-like 2–4 genes and their correlation with tumor-specific survival in a sex-specific manner and observed that different expression profiles were significantly correlated with survival in male and female patients. For male patients, decreased expression of Piwi-like 2/Piwi-like 3 was correlated with a 5.9-fold increase in the risk of tumor-related death (95% CI: 1.33–26.23; p = 0.02). In female patients, decreased Piwi-like 2/Piwi-like 4 expression was correlated with a 5.23-fold increase in the risk of tumor-related death (95% CI: 1.67–16.32; p = 0.004; Table 3 Figure 1). The results revealed that the combined expression of Piwi-like genes is correlated with tumor-specific survival in a sex-specific manner.


Expression of human Piwi-like genes is associated with prognosis for soft tissue sarcoma patients.

Greither T, Koser F, Kappler M, Bache M, Lautenschläger C, Göbel S, Holzhausen HJ, Wach S, Würl P, Taubert H - BMC Cancer (2012)

Multivariate Cox’s regression analyses: A. Combined expression of Piwi-like 2 and 3 in male STS patients and B. combined Piwi-like 2 and −4 expression in female STS patients and their correlation with tumor-specific survival.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3472179&req=5

Figure 1: Multivariate Cox’s regression analyses: A. Combined expression of Piwi-like 2 and 3 in male STS patients and B. combined Piwi-like 2 and −4 expression in female STS patients and their correlation with tumor-specific survival.
Mentions: Finally, we analyzed the combined expression of the Piwi-like 2–4 genes and their correlation with tumor-specific survival in a sex-specific manner and observed that different expression profiles were significantly correlated with survival in male and female patients. For male patients, decreased expression of Piwi-like 2/Piwi-like 3 was correlated with a 5.9-fold increase in the risk of tumor-related death (95% CI: 1.33–26.23; p = 0.02). In female patients, decreased Piwi-like 2/Piwi-like 4 expression was correlated with a 5.23-fold increase in the risk of tumor-related death (95% CI: 1.67–16.32; p = 0.004; Table 3 Figure 1). The results revealed that the combined expression of Piwi-like genes is correlated with tumor-specific survival in a sex-specific manner.

Bottom Line: In multivariate Cox's regression analyses, we showed that low Piwi-like 2 and Piwi-like 4 mRNA expression were significantly associated with a worse prognosis (RR = 1.87; p = 0.032 and RR = 1.82; p = 0.039).However, combined low Piwi-like 2 and 3 transcript levels were associated with worse survival (RR = 5.90; p = 0.02) for male patients.In this study, we identified a significant association between the expression of Piwi-like 2 and 4 mRNAs and the tumor-specific survival of soft tissue sarcoma patients.

View Article: PubMed Central - HTML - PubMed

Affiliation: Div, Molecular Urology, FAU Erlangen-Nürnberg, University Clinic of Urology, Erlangen, Germany.

ABSTRACT

Background: Argonaute genes are essential for RNA interference, stem cell maintenance and differentiation. The Piwi-like genes, a subclass of the Argonaute genes, are expressed mainly in the germline. These genes may be re-expressed in tumors, and expression of the Piwi-like genes is associated with prognosis in several types of tumors.

Methods: We measured the expression of Piwi-like mRNAs (Piwi-like 2-4) in 125 soft tissue sarcoma (STS) samples by qPCRs. Statistical tests were applied to study the correlation of expression levels with tumor-specific survival for STS patients.

Results: In multivariate Cox's regression analyses, we showed that low Piwi-like 2 and Piwi-like 4 mRNA expression were significantly associated with a worse prognosis (RR = 1.87; p = 0.032 and RR = 1.82; p = 0.039). Low expression of both genes was associated with a 2.58-fold increased risk of tumor-related death (p = 0.01). Piwi-like 4 and combined Piwi-like 2 and 4 mRNA levels correlated significantly with prognosis (RR = 3.53; p = 0.002 and RR = 5.23; p = 0.004) only for female but not for male patients. However, combined low Piwi-like 2 and 3 transcript levels were associated with worse survival (RR = 5.90; p = 0.02) for male patients.

Conclusions: In this study, we identified a significant association between the expression of Piwi-like 2 and 4 mRNAs and the tumor-specific survival of soft tissue sarcoma patients. Furthermore, a connection between sex and the impact of Piwi-like mRNA expressions on STS patients' prognosis was shown for the first time.

Show MeSH
Related in: MedlinePlus