Limits...
Allicin enhances host pro-inflammatory immune responses and protects against acute murine malaria infection.

Feng Y, Zhu X, Wang Q, Jiang Y, Shang H, Cui L, Cao Y - Malar. J. (2012)

Bottom Line: This effect is at least partially due to improved host immune responses.The absolute numbers of CD4+ T cells, DCs and macrophages were significantly higher in allicin-treated mice.In addition, allicin promoted the maturation of CD11c+ DCs, whereas it did not cause major changes in IL-4 and the level of anti-inflammatory cytokine IL-10.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Immunology, College of Basic Medical Sciences, China Medical University, Shenyang, Liaoning, China.

ABSTRACT

Background: During malaria infection, multiple pro-inflammatory mediators including IFN-γ, TNF and nitric oxide (NO) play a crucial role in the protection against the parasites. Modulation of host immunity is an important strategy to improve the outcome of malaria infection. Allicin is the major biologically active component of garlic and shows anti-microbial activity. Allicin is also active against protozoan parasites including Plasmodium, which is thought to be mediated by inhibiting cysteine proteases. In this study, the immunomodulatory activities of allicin were assessed during acute malaria infection using a rodent malaria model Plasmodium yoelii 17XL.

Methods: To determine whether allicin modulates host immune responses against malaria infection, mice were treated with allicin after infection with P. yoelii 17XL. Mortality was checked daily and parasitaemia was determined every other day. Pro-inflammatory mediators and IL-4 were quantified by ELISA, while NO level was determined by the Griess method. The populations of dendritic cells (DCs), macrophages, CD4+ T and regulatory T cells (Treg) were assessed by FACS.

Results: Allicin reduced parasitaemia and prolonged survival of the host in a dose-dependent manner. This effect is at least partially due to improved host immune responses. Results showed that allicin treatment enhanced the production of pro-inflammatory mediators such as IFN-γ, TNF, IL-12p70 and NO. The absolute numbers of CD4+ T cells, DCs and macrophages were significantly higher in allicin-treated mice. In addition, allicin promoted the maturation of CD11c+ DCs, whereas it did not cause major changes in IL-4 and the level of anti-inflammatory cytokine IL-10.

Conclusions: Allicin could partially protect host against P. yoelii 17XL through enhancement of the host innate and adaptive immune responses.

Show MeSH

Related in: MedlinePlus

Effects of allicin treatments on CD4+T cells and macrophages during P. yoelii 17XL infection. Absolute numbers of CD4+ T cells (A) and macrophages (B) were quantified by flow cytometry at day 0, 3 and 5 PI. One experiment representative of three is shown. Error bars represents SEM. Asterisks indicate statistically significant differences (*: P < 0.05; **: P < 0.01) between groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3472178&req=5

Figure 4: Effects of allicin treatments on CD4+T cells and macrophages during P. yoelii 17XL infection. Absolute numbers of CD4+ T cells (A) and macrophages (B) were quantified by flow cytometry at day 0, 3 and 5 PI. One experiment representative of three is shown. Error bars represents SEM. Asterisks indicate statistically significant differences (*: P < 0.05; **: P < 0.01) between groups.

Mentions: Protective immunity against blood-stage Plasmodium requires malaria-specific CD4+ T cells to rapidly and effectively control parasitaemia and clear the infection[47]. In addition, macrophages also play an essential role for parasite control during the early acute phase infection by the lethal P. yoelii strain[9]. Whereas significant changes in the number of CD4+ T cells in both allicin treatment groups on day 3 PI were observed, spleen CD4+ T cell expansion was detected on day 5 PI (Figure 4A). Again, this change in spleen CD4+ T cell was only evident in the 9 mg/kg allicin-treated group. Similarly, mice treated with 9 mg/kg of allicin had significantly more macrophages in the spleen than either control or 3 mg/kg allicin-treated mice (Figure 4B).


Allicin enhances host pro-inflammatory immune responses and protects against acute murine malaria infection.

Feng Y, Zhu X, Wang Q, Jiang Y, Shang H, Cui L, Cao Y - Malar. J. (2012)

Effects of allicin treatments on CD4+T cells and macrophages during P. yoelii 17XL infection. Absolute numbers of CD4+ T cells (A) and macrophages (B) were quantified by flow cytometry at day 0, 3 and 5 PI. One experiment representative of three is shown. Error bars represents SEM. Asterisks indicate statistically significant differences (*: P < 0.05; **: P < 0.01) between groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3472178&req=5

Figure 4: Effects of allicin treatments on CD4+T cells and macrophages during P. yoelii 17XL infection. Absolute numbers of CD4+ T cells (A) and macrophages (B) were quantified by flow cytometry at day 0, 3 and 5 PI. One experiment representative of three is shown. Error bars represents SEM. Asterisks indicate statistically significant differences (*: P < 0.05; **: P < 0.01) between groups.
Mentions: Protective immunity against blood-stage Plasmodium requires malaria-specific CD4+ T cells to rapidly and effectively control parasitaemia and clear the infection[47]. In addition, macrophages also play an essential role for parasite control during the early acute phase infection by the lethal P. yoelii strain[9]. Whereas significant changes in the number of CD4+ T cells in both allicin treatment groups on day 3 PI were observed, spleen CD4+ T cell expansion was detected on day 5 PI (Figure 4A). Again, this change in spleen CD4+ T cell was only evident in the 9 mg/kg allicin-treated group. Similarly, mice treated with 9 mg/kg of allicin had significantly more macrophages in the spleen than either control or 3 mg/kg allicin-treated mice (Figure 4B).

Bottom Line: This effect is at least partially due to improved host immune responses.The absolute numbers of CD4+ T cells, DCs and macrophages were significantly higher in allicin-treated mice.In addition, allicin promoted the maturation of CD11c+ DCs, whereas it did not cause major changes in IL-4 and the level of anti-inflammatory cytokine IL-10.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Immunology, College of Basic Medical Sciences, China Medical University, Shenyang, Liaoning, China.

ABSTRACT

Background: During malaria infection, multiple pro-inflammatory mediators including IFN-γ, TNF and nitric oxide (NO) play a crucial role in the protection against the parasites. Modulation of host immunity is an important strategy to improve the outcome of malaria infection. Allicin is the major biologically active component of garlic and shows anti-microbial activity. Allicin is also active against protozoan parasites including Plasmodium, which is thought to be mediated by inhibiting cysteine proteases. In this study, the immunomodulatory activities of allicin were assessed during acute malaria infection using a rodent malaria model Plasmodium yoelii 17XL.

Methods: To determine whether allicin modulates host immune responses against malaria infection, mice were treated with allicin after infection with P. yoelii 17XL. Mortality was checked daily and parasitaemia was determined every other day. Pro-inflammatory mediators and IL-4 were quantified by ELISA, while NO level was determined by the Griess method. The populations of dendritic cells (DCs), macrophages, CD4+ T and regulatory T cells (Treg) were assessed by FACS.

Results: Allicin reduced parasitaemia and prolonged survival of the host in a dose-dependent manner. This effect is at least partially due to improved host immune responses. Results showed that allicin treatment enhanced the production of pro-inflammatory mediators such as IFN-γ, TNF, IL-12p70 and NO. The absolute numbers of CD4+ T cells, DCs and macrophages were significantly higher in allicin-treated mice. In addition, allicin promoted the maturation of CD11c+ DCs, whereas it did not cause major changes in IL-4 and the level of anti-inflammatory cytokine IL-10.

Conclusions: Allicin could partially protect host against P. yoelii 17XL through enhancement of the host innate and adaptive immune responses.

Show MeSH
Related in: MedlinePlus