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Effects of high-fat diet on the numerical density and number of neuronal cells and the volume of the mouse hypothalamus: a stereological study.

Namavar MR, Raminfard S, Jahromi ZV, Azari H - Anat Cell Biol (2012)

Bottom Line: The numerical density and number of neuron and the volume of hypothalamus were estimated by using unbiased stereological methods.High fat diet decreased the neuronal cell density and increased the volume of the hypothalamus, but it did not significantly change its total neurons.These changes might be due to an increase in the extracellular space through inflammation or gliosis in the hypothalamus.

View Article: PubMed Central - PubMed

Affiliation: Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. ; Department of Anatomical Sciences, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

ABSTRACT
It has been demonstrated that the type of diet affects the brain structure and function. Consumption of fat-rich food is one of the most important factors that lead to increase in the prevalence of cardiovascular and neurological diseases. High-fat diet may change the volume and neuronal number or density in the hypothalamus, which is the center of energy control. Therefore, this study was designed to study the effect of high-fat diet on the density and number of neurons, and also the volume of hypothalamus in adult male mice. Forty male mice were divided into the control and experimental groups. The control group were fed with standard and the experimental groups, with high-fat diet for 4 (short-term) or 8 (long-term) weeks. The animals were perfused and brains were immediately removed, post-fixed and cut coronally and serially using cryostat at 30-µm thickness. Every 6th sections were stained by cresyl violet. The numerical density and number of neuron and the volume of hypothalamus were estimated by using unbiased stereological methods. Data analysis showed that both short and long time consumption of high-fat diet decreased the neuronal cell density of the hypothalamus. Interestingly, despite a decrease in the neuronal cell density, long time consumption of high-fat diet could significantly increase the volume of hypothalamus (P<0.05). High fat diet decreased the neuronal cell density and increased the volume of the hypothalamus, but it did not significantly change its total neurons. These changes might be due to an increase in the extracellular space through inflammation or gliosis in the hypothalamus.

No MeSH data available.


Related in: MedlinePlus

Mean value±SEM of numerical density of neuron (neuron/mm3) in total hypothalamus of mice were fed with normal and high fat diet (HFD) for short and long-term. Group: 1, short-term control; 2, long-term control; 3, short-term HFD; 4, long-term HFD. *Significant difference with short-term control group (P<0.005). †Significant difference with long-term control group (P<0.001).
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Figure 2: Mean value±SEM of numerical density of neuron (neuron/mm3) in total hypothalamus of mice were fed with normal and high fat diet (HFD) for short and long-term. Group: 1, short-term control; 2, long-term control; 3, short-term HFD; 4, long-term HFD. *Significant difference with short-term control group (P<0.005). †Significant difference with long-term control group (P<0.001).

Mentions: Table 3 and Fig. 2 shows that living for more 4 weeks in the control groups increased the numerical density of the neurons in the hypothalamus, but using HFD for more than 4 weeks decreased that parameter, although these changes were not significant. In addition, short-term HFD decreased the numerical density in both sides of the hypothalamus, although this decrease was not significant when compared with its control group (Table 3). After 8 weeks HFD, numerical density of the neurons significantly decreased when compared to the short-term (P<0.005) and the long-term (P<0.001) controls (Table 3, Fig. 2).


Effects of high-fat diet on the numerical density and number of neuronal cells and the volume of the mouse hypothalamus: a stereological study.

Namavar MR, Raminfard S, Jahromi ZV, Azari H - Anat Cell Biol (2012)

Mean value±SEM of numerical density of neuron (neuron/mm3) in total hypothalamus of mice were fed with normal and high fat diet (HFD) for short and long-term. Group: 1, short-term control; 2, long-term control; 3, short-term HFD; 4, long-term HFD. *Significant difference with short-term control group (P<0.005). †Significant difference with long-term control group (P<0.001).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3472144&req=5

Figure 2: Mean value±SEM of numerical density of neuron (neuron/mm3) in total hypothalamus of mice were fed with normal and high fat diet (HFD) for short and long-term. Group: 1, short-term control; 2, long-term control; 3, short-term HFD; 4, long-term HFD. *Significant difference with short-term control group (P<0.005). †Significant difference with long-term control group (P<0.001).
Mentions: Table 3 and Fig. 2 shows that living for more 4 weeks in the control groups increased the numerical density of the neurons in the hypothalamus, but using HFD for more than 4 weeks decreased that parameter, although these changes were not significant. In addition, short-term HFD decreased the numerical density in both sides of the hypothalamus, although this decrease was not significant when compared with its control group (Table 3). After 8 weeks HFD, numerical density of the neurons significantly decreased when compared to the short-term (P<0.005) and the long-term (P<0.001) controls (Table 3, Fig. 2).

Bottom Line: The numerical density and number of neuron and the volume of hypothalamus were estimated by using unbiased stereological methods.High fat diet decreased the neuronal cell density and increased the volume of the hypothalamus, but it did not significantly change its total neurons.These changes might be due to an increase in the extracellular space through inflammation or gliosis in the hypothalamus.

View Article: PubMed Central - PubMed

Affiliation: Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. ; Department of Anatomical Sciences, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

ABSTRACT
It has been demonstrated that the type of diet affects the brain structure and function. Consumption of fat-rich food is one of the most important factors that lead to increase in the prevalence of cardiovascular and neurological diseases. High-fat diet may change the volume and neuronal number or density in the hypothalamus, which is the center of energy control. Therefore, this study was designed to study the effect of high-fat diet on the density and number of neurons, and also the volume of hypothalamus in adult male mice. Forty male mice were divided into the control and experimental groups. The control group were fed with standard and the experimental groups, with high-fat diet for 4 (short-term) or 8 (long-term) weeks. The animals were perfused and brains were immediately removed, post-fixed and cut coronally and serially using cryostat at 30-µm thickness. Every 6th sections were stained by cresyl violet. The numerical density and number of neuron and the volume of hypothalamus were estimated by using unbiased stereological methods. Data analysis showed that both short and long time consumption of high-fat diet decreased the neuronal cell density of the hypothalamus. Interestingly, despite a decrease in the neuronal cell density, long time consumption of high-fat diet could significantly increase the volume of hypothalamus (P<0.05). High fat diet decreased the neuronal cell density and increased the volume of the hypothalamus, but it did not significantly change its total neurons. These changes might be due to an increase in the extracellular space through inflammation or gliosis in the hypothalamus.

No MeSH data available.


Related in: MedlinePlus