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Expression of Bis in the mouse gastrointestinal system.

Lee YD, Yoon JS, Yoon HH, Youn HJ, Kim J, Lee JH - Anat Cell Biol (2012)

Bottom Line: Ganglionated plexuses, located in submucous layers, as well as intermuscular layers, were specifically immunoreactive for Bis.Immunostaining with neuron specific esterase antibodies indicate that Bis is also present in the cell bodies of ganglions in the enteric nervous system (ENS).Our findings indicate that Bis plays a role in regulating GI functions, such as motility and absorption, through modulating signal transmission between the ENS and smooth muscles or the intestinal epitheliums.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, The Catholic University of Korea College of Medicine, Seoul, Korea.

ABSTRACT
The Bcl-2 interacting death suppressor (Bis) protein is known to be involved in a variety of pathophysiological conditions. We recently generated bis-deficient mice, which exhibited early lethality with typical nutritional deprivation status. To further investigate the molecular basis for the malnutrition phenotype of bis deficient mice, we explored Bis expression in the digestive system of normal mice. Western blot analysis and quantitative real time reverse transcription polymerase chain reaction analysis indicated that Bis expression is highest in the esophagus, followed by the stomach, colon, jejunum and ileum. Immunohistochemical data indicated that Bis expression is restricted to the stratified squamous epitheliums in the esophagus and forestomach, and was not notable in the columnar epitheliums in the stomach, small intestine and colon. In addition, strong Bis immunoreactivity was detected in the striated muscles surrounding the esophagus and smooth muscles at a lesser intensity throughout the gastrointestinal (GI) tract. Ganglionated plexuses, located in submucous layers, as well as intermuscular layers, were specifically immunoreactive for Bis. Immunofluorescence studies revealed that Bis is co-localized in glial fibrillary acidic protein-expressing enteric glial cells. Immunostaining with neuron specific esterase antibodies indicate that Bis is also present in the cell bodies of ganglions in the enteric nervous system (ENS). Our findings indicate that Bis plays a role in regulating GI functions, such as motility and absorption, through modulating signal transmission between the ENS and smooth muscles or the intestinal epitheliums.

No MeSH data available.


Related in: MedlinePlus

Immunohistochemical analysis of Bis in the esophagus (A) and stomach (B-D). Intense Bis immunoreactivity was observed in the stratified squamous epithelium and striated muscles (marked as white or black asterisk, respectively) in the esophagus (A). In epithelia of the grandular stomach, only trace levels of Bis expression were detected (B, C) and weak immunostaining for Bis was detected in the smooth muscles of the stomach, while specific localization of Bis was detected in myenteric plexus (arrows) (B, D). Higher magnifications of the boxed areas in B are shown in C and D. Scale bars=50 µm (A-D).
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Figure 2: Immunohistochemical analysis of Bis in the esophagus (A) and stomach (B-D). Intense Bis immunoreactivity was observed in the stratified squamous epithelium and striated muscles (marked as white or black asterisk, respectively) in the esophagus (A). In epithelia of the grandular stomach, only trace levels of Bis expression were detected (B, C) and weak immunostaining for Bis was detected in the smooth muscles of the stomach, while specific localization of Bis was detected in myenteric plexus (arrows) (B, D). Higher magnifications of the boxed areas in B are shown in C and D. Scale bars=50 µm (A-D).

Mentions: To identify the Bis expressing cells in the GI tract, we performed immunohistochemistry with tissue sections from each part of the mouse GI tract. An intense immunostaining for Bis was observed in the stratified squamous epithelium lining in the esophagus (Fig. 2A). In the stomach, Bis immunoreactivity was also prominently observed in the squamous epithelium of the forestomach, but this abruptly disappeared at the junction of the forestomach and glandular stomach, where the stratified squamous epithelium of the esophagus changes abruptly to the simple columnar epithelium of the stomach (Fig. 2B, C). Bis expression was also very low in the columnar epitheliums of the jejunum, ilum and colon (Fig. 3 and data not shown). Thus, Bis expression appears to be limited to the squamous epithelium and does not occur in the columnar epithelium in the GI tract. Bis has been reported to be strongly expressed in the skeletal and cardiac muscles [1]. In the GI tract, the striated muscles surrounding the esophagus also exhibit intense immunostaining for Bis (Fig. 2A). Although the intensity is weaker than in the striated muscles, Bis immunoreactivity was detected in the smooth muscles throughout the entire GI tract, from the esophagus to the colon, including the inner circular and outer longitudinal layers (Fig. 2B, D, 3). Therefore, the highest expression of Bis in the esophagus found in the western assay is likely due to a significant level of Bis expression in the squamous epitheliums and in the striated muscles.


Expression of Bis in the mouse gastrointestinal system.

Lee YD, Yoon JS, Yoon HH, Youn HJ, Kim J, Lee JH - Anat Cell Biol (2012)

Immunohistochemical analysis of Bis in the esophagus (A) and stomach (B-D). Intense Bis immunoreactivity was observed in the stratified squamous epithelium and striated muscles (marked as white or black asterisk, respectively) in the esophagus (A). In epithelia of the grandular stomach, only trace levels of Bis expression were detected (B, C) and weak immunostaining for Bis was detected in the smooth muscles of the stomach, while specific localization of Bis was detected in myenteric plexus (arrows) (B, D). Higher magnifications of the boxed areas in B are shown in C and D. Scale bars=50 µm (A-D).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3472142&req=5

Figure 2: Immunohistochemical analysis of Bis in the esophagus (A) and stomach (B-D). Intense Bis immunoreactivity was observed in the stratified squamous epithelium and striated muscles (marked as white or black asterisk, respectively) in the esophagus (A). In epithelia of the grandular stomach, only trace levels of Bis expression were detected (B, C) and weak immunostaining for Bis was detected in the smooth muscles of the stomach, while specific localization of Bis was detected in myenteric plexus (arrows) (B, D). Higher magnifications of the boxed areas in B are shown in C and D. Scale bars=50 µm (A-D).
Mentions: To identify the Bis expressing cells in the GI tract, we performed immunohistochemistry with tissue sections from each part of the mouse GI tract. An intense immunostaining for Bis was observed in the stratified squamous epithelium lining in the esophagus (Fig. 2A). In the stomach, Bis immunoreactivity was also prominently observed in the squamous epithelium of the forestomach, but this abruptly disappeared at the junction of the forestomach and glandular stomach, where the stratified squamous epithelium of the esophagus changes abruptly to the simple columnar epithelium of the stomach (Fig. 2B, C). Bis expression was also very low in the columnar epitheliums of the jejunum, ilum and colon (Fig. 3 and data not shown). Thus, Bis expression appears to be limited to the squamous epithelium and does not occur in the columnar epithelium in the GI tract. Bis has been reported to be strongly expressed in the skeletal and cardiac muscles [1]. In the GI tract, the striated muscles surrounding the esophagus also exhibit intense immunostaining for Bis (Fig. 2A). Although the intensity is weaker than in the striated muscles, Bis immunoreactivity was detected in the smooth muscles throughout the entire GI tract, from the esophagus to the colon, including the inner circular and outer longitudinal layers (Fig. 2B, D, 3). Therefore, the highest expression of Bis in the esophagus found in the western assay is likely due to a significant level of Bis expression in the squamous epitheliums and in the striated muscles.

Bottom Line: Ganglionated plexuses, located in submucous layers, as well as intermuscular layers, were specifically immunoreactive for Bis.Immunostaining with neuron specific esterase antibodies indicate that Bis is also present in the cell bodies of ganglions in the enteric nervous system (ENS).Our findings indicate that Bis plays a role in regulating GI functions, such as motility and absorption, through modulating signal transmission between the ENS and smooth muscles or the intestinal epitheliums.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, The Catholic University of Korea College of Medicine, Seoul, Korea.

ABSTRACT
The Bcl-2 interacting death suppressor (Bis) protein is known to be involved in a variety of pathophysiological conditions. We recently generated bis-deficient mice, which exhibited early lethality with typical nutritional deprivation status. To further investigate the molecular basis for the malnutrition phenotype of bis deficient mice, we explored Bis expression in the digestive system of normal mice. Western blot analysis and quantitative real time reverse transcription polymerase chain reaction analysis indicated that Bis expression is highest in the esophagus, followed by the stomach, colon, jejunum and ileum. Immunohistochemical data indicated that Bis expression is restricted to the stratified squamous epitheliums in the esophagus and forestomach, and was not notable in the columnar epitheliums in the stomach, small intestine and colon. In addition, strong Bis immunoreactivity was detected in the striated muscles surrounding the esophagus and smooth muscles at a lesser intensity throughout the gastrointestinal (GI) tract. Ganglionated plexuses, located in submucous layers, as well as intermuscular layers, were specifically immunoreactive for Bis. Immunofluorescence studies revealed that Bis is co-localized in glial fibrillary acidic protein-expressing enteric glial cells. Immunostaining with neuron specific esterase antibodies indicate that Bis is also present in the cell bodies of ganglions in the enteric nervous system (ENS). Our findings indicate that Bis plays a role in regulating GI functions, such as motility and absorption, through modulating signal transmission between the ENS and smooth muscles or the intestinal epitheliums.

No MeSH data available.


Related in: MedlinePlus