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TonEBP and SMIT expression in human placenta.

Shin JA, Kwon HM, Han KH, Lee HY - Anat Cell Biol (2012)

Bottom Line: Tonicity-responsive enhancer binding protein (TonEBP) is a signal transcription factor of transporters such as sodium-myo-inositol cotransporter (SMIT), aldose reductase.This shows TonEBP is a key factor in SMIT transcription.TonEBP may play an important role in transporting of inositol to fetus in placenta.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Ewha Womans University School of Medicine, Seoul, Korea.

ABSTRACT
Tonicity-responsive enhancer binding protein (TonEBP) is a signal transcription factor of transporters such as sodium-myo-inositol cotransporter (SMIT), aldose reductase. TonEBP has a variety of functions such as control of intracellular osmolytes and immunomodulating. It is known that TonEBP is abundant in the placenta, but location and function aren't known. The aim of this study is to describe the localization of TonEBP in the placenta. We assayed the immunohistochemistry of TonEBP and performed in situ hybridization of SMIT in normal human full term placenta. In normal human full term placenta, TonEBP was in villous trophoblasts, extravillous trophoblasts and some endothelial cells. The result of the in situ hybridization of SMIT was similar to that of immunohistochemistry of TonEBP. Neither TonEBP nor SMIT was present in TonEBP knockout mouse placenta. This shows TonEBP is a key factor in SMIT transcription. TonEBP may play an important role in transporting of inositol to fetus in placenta.

No MeSH data available.


Related in: MedlinePlus

Immunohistochemical localization of tonicity-responsive enhancer binding protein (TonEBP) and in situ hybridization of sodium-myo-inositol cotransporter (SMIT) in TonEBP knockout mouse. (A) Nucleus of labyrinthin trophoblasts were stained for TonEBP immunohistochemistry in wild type mouse placenta (arrows). (B) No specific staining of TonEBP was seen in TonEBP knockout mouse placenta (arrowheads). (C) Cytoplasm of labyrinthin trophoblasts were stained for SMIT in situ hybridization in wild type mouse placenta (arrows). (D) No specific staining of SMIT was seen in TonEBP knockout mouse placenta (arrowheads). Scale bars=50 µm (A-D).
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Figure 3: Immunohistochemical localization of tonicity-responsive enhancer binding protein (TonEBP) and in situ hybridization of sodium-myo-inositol cotransporter (SMIT) in TonEBP knockout mouse. (A) Nucleus of labyrinthin trophoblasts were stained for TonEBP immunohistochemistry in wild type mouse placenta (arrows). (B) No specific staining of TonEBP was seen in TonEBP knockout mouse placenta (arrowheads). (C) Cytoplasm of labyrinthin trophoblasts were stained for SMIT in situ hybridization in wild type mouse placenta (arrows). (D) No specific staining of SMIT was seen in TonEBP knockout mouse placenta (arrowheads). Scale bars=50 µm (A-D).

Mentions: To make clear the relation between TonEBP and SMIT, immunohistochemistry of TonEBP and in situ hybridization of SMIT was carried out in TonEBP knockout mouse placenta. Both TonEBP and SMIT were showed positive signal in labyrinthine trophoblasts of wild type mouse (Fig. 3A, C). As expected, TonEBP expression was not detected in TonEBP knockout mouse and SMIT mRNA was also absent in the TonEBP knockout mouse (Fig. 3B, D).


TonEBP and SMIT expression in human placenta.

Shin JA, Kwon HM, Han KH, Lee HY - Anat Cell Biol (2012)

Immunohistochemical localization of tonicity-responsive enhancer binding protein (TonEBP) and in situ hybridization of sodium-myo-inositol cotransporter (SMIT) in TonEBP knockout mouse. (A) Nucleus of labyrinthin trophoblasts were stained for TonEBP immunohistochemistry in wild type mouse placenta (arrows). (B) No specific staining of TonEBP was seen in TonEBP knockout mouse placenta (arrowheads). (C) Cytoplasm of labyrinthin trophoblasts were stained for SMIT in situ hybridization in wild type mouse placenta (arrows). (D) No specific staining of SMIT was seen in TonEBP knockout mouse placenta (arrowheads). Scale bars=50 µm (A-D).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3472141&req=5

Figure 3: Immunohistochemical localization of tonicity-responsive enhancer binding protein (TonEBP) and in situ hybridization of sodium-myo-inositol cotransporter (SMIT) in TonEBP knockout mouse. (A) Nucleus of labyrinthin trophoblasts were stained for TonEBP immunohistochemistry in wild type mouse placenta (arrows). (B) No specific staining of TonEBP was seen in TonEBP knockout mouse placenta (arrowheads). (C) Cytoplasm of labyrinthin trophoblasts were stained for SMIT in situ hybridization in wild type mouse placenta (arrows). (D) No specific staining of SMIT was seen in TonEBP knockout mouse placenta (arrowheads). Scale bars=50 µm (A-D).
Mentions: To make clear the relation between TonEBP and SMIT, immunohistochemistry of TonEBP and in situ hybridization of SMIT was carried out in TonEBP knockout mouse placenta. Both TonEBP and SMIT were showed positive signal in labyrinthine trophoblasts of wild type mouse (Fig. 3A, C). As expected, TonEBP expression was not detected in TonEBP knockout mouse and SMIT mRNA was also absent in the TonEBP knockout mouse (Fig. 3B, D).

Bottom Line: Tonicity-responsive enhancer binding protein (TonEBP) is a signal transcription factor of transporters such as sodium-myo-inositol cotransporter (SMIT), aldose reductase.This shows TonEBP is a key factor in SMIT transcription.TonEBP may play an important role in transporting of inositol to fetus in placenta.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Ewha Womans University School of Medicine, Seoul, Korea.

ABSTRACT
Tonicity-responsive enhancer binding protein (TonEBP) is a signal transcription factor of transporters such as sodium-myo-inositol cotransporter (SMIT), aldose reductase. TonEBP has a variety of functions such as control of intracellular osmolytes and immunomodulating. It is known that TonEBP is abundant in the placenta, but location and function aren't known. The aim of this study is to describe the localization of TonEBP in the placenta. We assayed the immunohistochemistry of TonEBP and performed in situ hybridization of SMIT in normal human full term placenta. In normal human full term placenta, TonEBP was in villous trophoblasts, extravillous trophoblasts and some endothelial cells. The result of the in situ hybridization of SMIT was similar to that of immunohistochemistry of TonEBP. Neither TonEBP nor SMIT was present in TonEBP knockout mouse placenta. This shows TonEBP is a key factor in SMIT transcription. TonEBP may play an important role in transporting of inositol to fetus in placenta.

No MeSH data available.


Related in: MedlinePlus