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TonEBP and SMIT expression in human placenta.

Shin JA, Kwon HM, Han KH, Lee HY - Anat Cell Biol (2012)

Bottom Line: Tonicity-responsive enhancer binding protein (TonEBP) is a signal transcription factor of transporters such as sodium-myo-inositol cotransporter (SMIT), aldose reductase.This shows TonEBP is a key factor in SMIT transcription.TonEBP may play an important role in transporting of inositol to fetus in placenta.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Ewha Womans University School of Medicine, Seoul, Korea.

ABSTRACT
Tonicity-responsive enhancer binding protein (TonEBP) is a signal transcription factor of transporters such as sodium-myo-inositol cotransporter (SMIT), aldose reductase. TonEBP has a variety of functions such as control of intracellular osmolytes and immunomodulating. It is known that TonEBP is abundant in the placenta, but location and function aren't known. The aim of this study is to describe the localization of TonEBP in the placenta. We assayed the immunohistochemistry of TonEBP and performed in situ hybridization of SMIT in normal human full term placenta. In normal human full term placenta, TonEBP was in villous trophoblasts, extravillous trophoblasts and some endothelial cells. The result of the in situ hybridization of SMIT was similar to that of immunohistochemistry of TonEBP. Neither TonEBP nor SMIT was present in TonEBP knockout mouse placenta. This shows TonEBP is a key factor in SMIT transcription. TonEBP may play an important role in transporting of inositol to fetus in placenta.

No MeSH data available.


Related in: MedlinePlus

In situ hybridization of sodium-myo-inositol cotransporter (SMIT) in human placenta. (A, B) Cytoplasmic staining of SMIT antisense riboprobe was mainly in the villous trophoblast (arrows). Some endothelial cells and connective cells were weakly stained. (C) As a negative control, no specific staining of SMIT sense riboprobe was seen. Scale bars=50 µm (A-C).
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Figure 2: In situ hybridization of sodium-myo-inositol cotransporter (SMIT) in human placenta. (A, B) Cytoplasmic staining of SMIT antisense riboprobe was mainly in the villous trophoblast (arrows). Some endothelial cells and connective cells were weakly stained. (C) As a negative control, no specific staining of SMIT sense riboprobe was seen. Scale bars=50 µm (A-C).

Mentions: Expression of SMIT mRNA was examined by in situ hybridization of the antisense riboprobe. The sense riboprobe did not produce significant hybridization (Fig. 2C). SMIT mRNA was strongly detected in the cytoplasm of trophoblasts and weakly in endothelial cells (Table 1, Fig. 2A, B). The localization of SMIT was similar to that of TonEBP.


TonEBP and SMIT expression in human placenta.

Shin JA, Kwon HM, Han KH, Lee HY - Anat Cell Biol (2012)

In situ hybridization of sodium-myo-inositol cotransporter (SMIT) in human placenta. (A, B) Cytoplasmic staining of SMIT antisense riboprobe was mainly in the villous trophoblast (arrows). Some endothelial cells and connective cells were weakly stained. (C) As a negative control, no specific staining of SMIT sense riboprobe was seen. Scale bars=50 µm (A-C).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3472141&req=5

Figure 2: In situ hybridization of sodium-myo-inositol cotransporter (SMIT) in human placenta. (A, B) Cytoplasmic staining of SMIT antisense riboprobe was mainly in the villous trophoblast (arrows). Some endothelial cells and connective cells were weakly stained. (C) As a negative control, no specific staining of SMIT sense riboprobe was seen. Scale bars=50 µm (A-C).
Mentions: Expression of SMIT mRNA was examined by in situ hybridization of the antisense riboprobe. The sense riboprobe did not produce significant hybridization (Fig. 2C). SMIT mRNA was strongly detected in the cytoplasm of trophoblasts and weakly in endothelial cells (Table 1, Fig. 2A, B). The localization of SMIT was similar to that of TonEBP.

Bottom Line: Tonicity-responsive enhancer binding protein (TonEBP) is a signal transcription factor of transporters such as sodium-myo-inositol cotransporter (SMIT), aldose reductase.This shows TonEBP is a key factor in SMIT transcription.TonEBP may play an important role in transporting of inositol to fetus in placenta.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Ewha Womans University School of Medicine, Seoul, Korea.

ABSTRACT
Tonicity-responsive enhancer binding protein (TonEBP) is a signal transcription factor of transporters such as sodium-myo-inositol cotransporter (SMIT), aldose reductase. TonEBP has a variety of functions such as control of intracellular osmolytes and immunomodulating. It is known that TonEBP is abundant in the placenta, but location and function aren't known. The aim of this study is to describe the localization of TonEBP in the placenta. We assayed the immunohistochemistry of TonEBP and performed in situ hybridization of SMIT in normal human full term placenta. In normal human full term placenta, TonEBP was in villous trophoblasts, extravillous trophoblasts and some endothelial cells. The result of the in situ hybridization of SMIT was similar to that of immunohistochemistry of TonEBP. Neither TonEBP nor SMIT was present in TonEBP knockout mouse placenta. This shows TonEBP is a key factor in SMIT transcription. TonEBP may play an important role in transporting of inositol to fetus in placenta.

No MeSH data available.


Related in: MedlinePlus