Limits...
TonEBP and SMIT expression in human placenta.

Shin JA, Kwon HM, Han KH, Lee HY - Anat Cell Biol (2012)

Bottom Line: Tonicity-responsive enhancer binding protein (TonEBP) is a signal transcription factor of transporters such as sodium-myo-inositol cotransporter (SMIT), aldose reductase.This shows TonEBP is a key factor in SMIT transcription.TonEBP may play an important role in transporting of inositol to fetus in placenta.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Ewha Womans University School of Medicine, Seoul, Korea.

ABSTRACT
Tonicity-responsive enhancer binding protein (TonEBP) is a signal transcription factor of transporters such as sodium-myo-inositol cotransporter (SMIT), aldose reductase. TonEBP has a variety of functions such as control of intracellular osmolytes and immunomodulating. It is known that TonEBP is abundant in the placenta, but location and function aren't known. The aim of this study is to describe the localization of TonEBP in the placenta. We assayed the immunohistochemistry of TonEBP and performed in situ hybridization of SMIT in normal human full term placenta. In normal human full term placenta, TonEBP was in villous trophoblasts, extravillous trophoblasts and some endothelial cells. The result of the in situ hybridization of SMIT was similar to that of immunohistochemistry of TonEBP. Neither TonEBP nor SMIT was present in TonEBP knockout mouse placenta. This shows TonEBP is a key factor in SMIT transcription. TonEBP may play an important role in transporting of inositol to fetus in placenta.

No MeSH data available.


Related in: MedlinePlus

Immunohistochemical localization of tonicity-responsive enhancer binding protein (TonEBP) in human placenta. (A) Nuclear staining of TonEBP was mainly in the villous trophoblasts (arrows). Some endothelial cells and connective cells were weakly stained. (B) TonEBP stained cell mass was showed in the basal plate (arrows). (C) Nuclear TonEBP stained cells (brown) were colocalized with cytokeratin 7 (blue). (D) Nuclear TonEBP stained cells (brown) were not colocalized with vimentin (blue). Serial 3 µm section of (C). (E) Negative control showed no labeling. Scale bars=50 µm (A-E).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3472141&req=5

Figure 1: Immunohistochemical localization of tonicity-responsive enhancer binding protein (TonEBP) in human placenta. (A) Nuclear staining of TonEBP was mainly in the villous trophoblasts (arrows). Some endothelial cells and connective cells were weakly stained. (B) TonEBP stained cell mass was showed in the basal plate (arrows). (C) Nuclear TonEBP stained cells (brown) were colocalized with cytokeratin 7 (blue). (D) Nuclear TonEBP stained cells (brown) were not colocalized with vimentin (blue). Serial 3 µm section of (C). (E) Negative control showed no labeling. Scale bars=50 µm (A-E).

Mentions: TonEBP mRNA is most abundant in placenta [6]. We performed immunohistochemistry for TonEBP in normal human full term placenta and confirmed expression of TonEBP. TonEBP staining was strong in the nucleus of villous trophoblasts. Some endothelial cells and other connective cells were weakly stained (Table 1, Fig. 1A). TonEBP was also detectable in a cell mass of basal plate (Fig. 1B). Localization of TonEBP was confirmed with various cell markers, cytokeratin 7 and vimentin in serial section (3 µm) of paraffin block. The TonEBP positive cell mass in basal plate colocalized with cytokeratin 7 but not with vimentin (Fig. 1C, D). Cytokeratin 7 is a marker for extravillous trophoblasts [14]. TonEBP immunoreactivity was mainly in villous and extravillous trophoblasts. The placenta stained with preimmune serum did not produce significant staining (Fig. 1E).


TonEBP and SMIT expression in human placenta.

Shin JA, Kwon HM, Han KH, Lee HY - Anat Cell Biol (2012)

Immunohistochemical localization of tonicity-responsive enhancer binding protein (TonEBP) in human placenta. (A) Nuclear staining of TonEBP was mainly in the villous trophoblasts (arrows). Some endothelial cells and connective cells were weakly stained. (B) TonEBP stained cell mass was showed in the basal plate (arrows). (C) Nuclear TonEBP stained cells (brown) were colocalized with cytokeratin 7 (blue). (D) Nuclear TonEBP stained cells (brown) were not colocalized with vimentin (blue). Serial 3 µm section of (C). (E) Negative control showed no labeling. Scale bars=50 µm (A-E).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3472141&req=5

Figure 1: Immunohistochemical localization of tonicity-responsive enhancer binding protein (TonEBP) in human placenta. (A) Nuclear staining of TonEBP was mainly in the villous trophoblasts (arrows). Some endothelial cells and connective cells were weakly stained. (B) TonEBP stained cell mass was showed in the basal plate (arrows). (C) Nuclear TonEBP stained cells (brown) were colocalized with cytokeratin 7 (blue). (D) Nuclear TonEBP stained cells (brown) were not colocalized with vimentin (blue). Serial 3 µm section of (C). (E) Negative control showed no labeling. Scale bars=50 µm (A-E).
Mentions: TonEBP mRNA is most abundant in placenta [6]. We performed immunohistochemistry for TonEBP in normal human full term placenta and confirmed expression of TonEBP. TonEBP staining was strong in the nucleus of villous trophoblasts. Some endothelial cells and other connective cells were weakly stained (Table 1, Fig. 1A). TonEBP was also detectable in a cell mass of basal plate (Fig. 1B). Localization of TonEBP was confirmed with various cell markers, cytokeratin 7 and vimentin in serial section (3 µm) of paraffin block. The TonEBP positive cell mass in basal plate colocalized with cytokeratin 7 but not with vimentin (Fig. 1C, D). Cytokeratin 7 is a marker for extravillous trophoblasts [14]. TonEBP immunoreactivity was mainly in villous and extravillous trophoblasts. The placenta stained with preimmune serum did not produce significant staining (Fig. 1E).

Bottom Line: Tonicity-responsive enhancer binding protein (TonEBP) is a signal transcription factor of transporters such as sodium-myo-inositol cotransporter (SMIT), aldose reductase.This shows TonEBP is a key factor in SMIT transcription.TonEBP may play an important role in transporting of inositol to fetus in placenta.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Ewha Womans University School of Medicine, Seoul, Korea.

ABSTRACT
Tonicity-responsive enhancer binding protein (TonEBP) is a signal transcription factor of transporters such as sodium-myo-inositol cotransporter (SMIT), aldose reductase. TonEBP has a variety of functions such as control of intracellular osmolytes and immunomodulating. It is known that TonEBP is abundant in the placenta, but location and function aren't known. The aim of this study is to describe the localization of TonEBP in the placenta. We assayed the immunohistochemistry of TonEBP and performed in situ hybridization of SMIT in normal human full term placenta. In normal human full term placenta, TonEBP was in villous trophoblasts, extravillous trophoblasts and some endothelial cells. The result of the in situ hybridization of SMIT was similar to that of immunohistochemistry of TonEBP. Neither TonEBP nor SMIT was present in TonEBP knockout mouse placenta. This shows TonEBP is a key factor in SMIT transcription. TonEBP may play an important role in transporting of inositol to fetus in placenta.

No MeSH data available.


Related in: MedlinePlus