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Prostate stem cell antigen gene is expressed in islets of pancreas.

Ono H, Yanagihara K, Sakamoto H, Yoshida T, Saeki N - Anat Cell Biol (2012)

Bottom Line: Prostate stem cell antigen (PSCA) is a glycosylphosphatidylinositol-anchored cell surface antigen with an organ-dependent expression pattern in cancers; e.g., up-regulated in prostate cancer and down-regulated in gastric cancer.In our investigation of the transcription start site of PSCA, we found a non-coding splicing variant of PSCA as well as authentic PSCA transcripts in mRNA samples from a normal pancreas.We previously reported that PSCA expression is correlated to the methylation status of the enhancer region in gastric and gallbladder cancer cell lines but not in pancreatic cancer cell lines, suggesting that PSCA expression is regulated in a diff erent mode in pancreatic cancer from that in gastric and gallbladder cancers.

View Article: PubMed Central - PubMed

Affiliation: Division of Genetics, National Cancer Center Research Institute, Tokyo, Japan.

ABSTRACT
Prostate stem cell antigen (PSCA) is a glycosylphosphatidylinositol-anchored cell surface antigen with an organ-dependent expression pattern in cancers; e.g., up-regulated in prostate cancer and down-regulated in gastric cancer. Previously it was reported that PSCA is not expressed in the normal pancreas but aberrantly expressed in pancreatic cancer. In this present study, we identified PSCA expression in islets of the pancreas by immunohistochemistry, which was co-localized with four islet-cell markers: insulin, glucagon, somatostatin and pancreatic polypeptide. In our investigation of the transcription start site of PSCA, we found a non-coding splicing variant of PSCA as well as authentic PSCA transcripts in mRNA samples from a normal pancreas. Both the transcripts were also identified in several pancreatic cancer cell lines. We previously reported that PSCA expression is correlated to the methylation status of the enhancer region in gastric and gallbladder cancer cell lines but not in pancreatic cancer cell lines, suggesting that PSCA expression is regulated in a diff erent mode in pancreatic cancer from that in gastric and gallbladder cancers.

No MeSH data available.


Related in: MedlinePlus

Two splicing variants of the prostate stem cell antigen (PSCA) transcript are expressed in normal pancreatic, gastric and gallbladder tissues and in cancer cell lines derived from their cancers. (A) Quantitative reverse transcription polymerase chain reaction (RT-PCR) revealed PSCA transcript (variant 1) in human normal pancreas. Colon is negative control as PSCA is not expressed in that organ. (B) Schematic representation of the structure of the two variants. Position in chromosome 8q24 is based on NCBI Build 37.3. (C) Quantitative RT-PCR revealed expression of variants 1 and 2 in the normal tissues and the cancer cell lines, and variant 2 is dominant in the pancreas and pancreatic cancer cell lines.
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Figure 2: Two splicing variants of the prostate stem cell antigen (PSCA) transcript are expressed in normal pancreatic, gastric and gallbladder tissues and in cancer cell lines derived from their cancers. (A) Quantitative reverse transcription polymerase chain reaction (RT-PCR) revealed PSCA transcript (variant 1) in human normal pancreas. Colon is negative control as PSCA is not expressed in that organ. (B) Schematic representation of the structure of the two variants. Position in chromosome 8q24 is based on NCBI Build 37.3. (C) Quantitative RT-PCR revealed expression of variants 1 and 2 in the normal tissues and the cancer cell lines, and variant 2 is dominant in the pancreas and pancreatic cancer cell lines.

Mentions: The templates were synthesized from commercially available total RNA isolated from normal human pancreas, stomach and gallbladder (BioChain), or total RNA isolated from the pancreatic, gastric and bladder cancer cell lines, using the ThermoScript RT-PCR System (Invitrogen). Quantitative RT-PCR in Figs. 2A and 3A was performed by TaqMan Gene Expression Assay (Life Technologies Japan, Tokyo, Japan; Applied Biosystems assay ID: Hs00194665_m1 for PSCA, Applied Biosystems Part No. 4326317E for glyceraldehyde-3-phosphate dehydrogenase [GAPDH]), which was conducted for 40 cycles under a condition of 2 steps of temperature: 95℃ for 15 seconds and 60℃ for 60 seconds, by ABI PRISM 7900HT Sequence Detection System (Life Technologies Japan). Quantitative RT-PCR in Fig. 2C was performed with gene expression assay using SYBR Premix Ex Taq II (Takara Bio Inc., Shiga, Japan), conducted in 40 cycles under a condition of 2 steps of temperature: 95℃ for 5 seconds and 60℃ for 30 seconds, by the ABI PRISM 7900HT Sequence Detection System. The relative transcript level was calculated using the Ct value of GAPDH transcript as reference.


Prostate stem cell antigen gene is expressed in islets of pancreas.

Ono H, Yanagihara K, Sakamoto H, Yoshida T, Saeki N - Anat Cell Biol (2012)

Two splicing variants of the prostate stem cell antigen (PSCA) transcript are expressed in normal pancreatic, gastric and gallbladder tissues and in cancer cell lines derived from their cancers. (A) Quantitative reverse transcription polymerase chain reaction (RT-PCR) revealed PSCA transcript (variant 1) in human normal pancreas. Colon is negative control as PSCA is not expressed in that organ. (B) Schematic representation of the structure of the two variants. Position in chromosome 8q24 is based on NCBI Build 37.3. (C) Quantitative RT-PCR revealed expression of variants 1 and 2 in the normal tissues and the cancer cell lines, and variant 2 is dominant in the pancreas and pancreatic cancer cell lines.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3472140&req=5

Figure 2: Two splicing variants of the prostate stem cell antigen (PSCA) transcript are expressed in normal pancreatic, gastric and gallbladder tissues and in cancer cell lines derived from their cancers. (A) Quantitative reverse transcription polymerase chain reaction (RT-PCR) revealed PSCA transcript (variant 1) in human normal pancreas. Colon is negative control as PSCA is not expressed in that organ. (B) Schematic representation of the structure of the two variants. Position in chromosome 8q24 is based on NCBI Build 37.3. (C) Quantitative RT-PCR revealed expression of variants 1 and 2 in the normal tissues and the cancer cell lines, and variant 2 is dominant in the pancreas and pancreatic cancer cell lines.
Mentions: The templates were synthesized from commercially available total RNA isolated from normal human pancreas, stomach and gallbladder (BioChain), or total RNA isolated from the pancreatic, gastric and bladder cancer cell lines, using the ThermoScript RT-PCR System (Invitrogen). Quantitative RT-PCR in Figs. 2A and 3A was performed by TaqMan Gene Expression Assay (Life Technologies Japan, Tokyo, Japan; Applied Biosystems assay ID: Hs00194665_m1 for PSCA, Applied Biosystems Part No. 4326317E for glyceraldehyde-3-phosphate dehydrogenase [GAPDH]), which was conducted for 40 cycles under a condition of 2 steps of temperature: 95℃ for 15 seconds and 60℃ for 60 seconds, by ABI PRISM 7900HT Sequence Detection System (Life Technologies Japan). Quantitative RT-PCR in Fig. 2C was performed with gene expression assay using SYBR Premix Ex Taq II (Takara Bio Inc., Shiga, Japan), conducted in 40 cycles under a condition of 2 steps of temperature: 95℃ for 5 seconds and 60℃ for 30 seconds, by the ABI PRISM 7900HT Sequence Detection System. The relative transcript level was calculated using the Ct value of GAPDH transcript as reference.

Bottom Line: Prostate stem cell antigen (PSCA) is a glycosylphosphatidylinositol-anchored cell surface antigen with an organ-dependent expression pattern in cancers; e.g., up-regulated in prostate cancer and down-regulated in gastric cancer.In our investigation of the transcription start site of PSCA, we found a non-coding splicing variant of PSCA as well as authentic PSCA transcripts in mRNA samples from a normal pancreas.We previously reported that PSCA expression is correlated to the methylation status of the enhancer region in gastric and gallbladder cancer cell lines but not in pancreatic cancer cell lines, suggesting that PSCA expression is regulated in a diff erent mode in pancreatic cancer from that in gastric and gallbladder cancers.

View Article: PubMed Central - PubMed

Affiliation: Division of Genetics, National Cancer Center Research Institute, Tokyo, Japan.

ABSTRACT
Prostate stem cell antigen (PSCA) is a glycosylphosphatidylinositol-anchored cell surface antigen with an organ-dependent expression pattern in cancers; e.g., up-regulated in prostate cancer and down-regulated in gastric cancer. Previously it was reported that PSCA is not expressed in the normal pancreas but aberrantly expressed in pancreatic cancer. In this present study, we identified PSCA expression in islets of the pancreas by immunohistochemistry, which was co-localized with four islet-cell markers: insulin, glucagon, somatostatin and pancreatic polypeptide. In our investigation of the transcription start site of PSCA, we found a non-coding splicing variant of PSCA as well as authentic PSCA transcripts in mRNA samples from a normal pancreas. Both the transcripts were also identified in several pancreatic cancer cell lines. We previously reported that PSCA expression is correlated to the methylation status of the enhancer region in gastric and gallbladder cancer cell lines but not in pancreatic cancer cell lines, suggesting that PSCA expression is regulated in a diff erent mode in pancreatic cancer from that in gastric and gallbladder cancers.

No MeSH data available.


Related in: MedlinePlus