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Prostate stem cell antigen gene is expressed in islets of pancreas.

Ono H, Yanagihara K, Sakamoto H, Yoshida T, Saeki N - Anat Cell Biol (2012)

Bottom Line: Prostate stem cell antigen (PSCA) is a glycosylphosphatidylinositol-anchored cell surface antigen with an organ-dependent expression pattern in cancers; e.g., up-regulated in prostate cancer and down-regulated in gastric cancer.In our investigation of the transcription start site of PSCA, we found a non-coding splicing variant of PSCA as well as authentic PSCA transcripts in mRNA samples from a normal pancreas.We previously reported that PSCA expression is correlated to the methylation status of the enhancer region in gastric and gallbladder cancer cell lines but not in pancreatic cancer cell lines, suggesting that PSCA expression is regulated in a diff erent mode in pancreatic cancer from that in gastric and gallbladder cancers.

View Article: PubMed Central - PubMed

Affiliation: Division of Genetics, National Cancer Center Research Institute, Tokyo, Japan.

ABSTRACT
Prostate stem cell antigen (PSCA) is a glycosylphosphatidylinositol-anchored cell surface antigen with an organ-dependent expression pattern in cancers; e.g., up-regulated in prostate cancer and down-regulated in gastric cancer. Previously it was reported that PSCA is not expressed in the normal pancreas but aberrantly expressed in pancreatic cancer. In this present study, we identified PSCA expression in islets of the pancreas by immunohistochemistry, which was co-localized with four islet-cell markers: insulin, glucagon, somatostatin and pancreatic polypeptide. In our investigation of the transcription start site of PSCA, we found a non-coding splicing variant of PSCA as well as authentic PSCA transcripts in mRNA samples from a normal pancreas. Both the transcripts were also identified in several pancreatic cancer cell lines. We previously reported that PSCA expression is correlated to the methylation status of the enhancer region in gastric and gallbladder cancer cell lines but not in pancreatic cancer cell lines, suggesting that PSCA expression is regulated in a diff erent mode in pancreatic cancer from that in gastric and gallbladder cancers.

No MeSH data available.


Related in: MedlinePlus

Prostate stem cell antigen (PSCA) is expressed in the islet of human normal pancreas (immunohistochemistry). (A) Double staining with anti-PSCA (blue) and anti-proliferating cell nuclear (brown) antibodies. The right panel is magnification of the islet shown in the left column. (B) Fluorescent double staining of anti-PSCA antibody (red) and four islet-cell markers (blue), insulin (β cell), glucagon (α cell), somatostatin (δ cell) or pancreatic polypeptide (PP cell). Each series of the image was obtained by focusing on the islet-cell markers.
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Figure 1: Prostate stem cell antigen (PSCA) is expressed in the islet of human normal pancreas (immunohistochemistry). (A) Double staining with anti-PSCA (blue) and anti-proliferating cell nuclear (brown) antibodies. The right panel is magnification of the islet shown in the left column. (B) Fluorescent double staining of anti-PSCA antibody (red) and four islet-cell markers (blue), insulin (β cell), glucagon (α cell), somatostatin (δ cell) or pancreatic polypeptide (PP cell). Each series of the image was obtained by focusing on the islet-cell markers.

Mentions: Embedded normal pancreas specimens on glass were purchased from BioChain Institute (Newark, CA, USA). Immunohistochemistry (Fig. 1A) was performed by the same procedure as described in the previous studies [4, 5] with mouse monoclonal anti-PSCA antibody whose specificity had been proved previously [4], rabbit anti-proliferating cell nuclear antigen (PCNA) antibody (sc-7907, Santa Cruz Biotechnology, Santa Cruz, CA, USA). The sections were incubated at 4℃ overnight with both antibodies simultaneously, and then with alkaline phosphatase conjugated anti-mouse IgG antibody and peroxidase conjugated anti-rabbit IgG antibody (NICHIREI Biosciences Inc., Tokyo, Japan) for an hour at room temperature. PSCA protein was visualized by Alkaline Phosphatase Substrate Kit III (Vector Laboratories, Burlingame, CA, USA) and PCNA by Vector NovaRED Substrate Kit for Peroxidase (Vector Laboratories). The fluorescent immunohistochemistry (Fig. 1B) was conducted using anti-PSCA, rabbit anti-insulin (sc-9186, Santa Cruz Biotechnology), rabbit anti-glucagon (ab18461, Abcam Japan, Tokyo, Japan), rabbit anti-somatostatin (A0566, Dako Japan, Tokyo, Japan) and goat anti-pancreatic polypeptide (NB100-1793, Novus Biologicals, Littleton, CO, USA) antibodies for primary antibodies, and anti-mouse IgG antibody-Rhodamin, anti-rabbit IgG-FITC and anti-goat IgG-FITC conjugates (sc-2300, sc-2090, sc-2024, respectively, Santa Cruz Biotechnology) for secondary antibodies. The fluorescent signals were obtained by Zeiss LSM510 META Confocal Imaging System (Carl Zeiss MicroImaging, LLC, Thornwood, NY, USA).


Prostate stem cell antigen gene is expressed in islets of pancreas.

Ono H, Yanagihara K, Sakamoto H, Yoshida T, Saeki N - Anat Cell Biol (2012)

Prostate stem cell antigen (PSCA) is expressed in the islet of human normal pancreas (immunohistochemistry). (A) Double staining with anti-PSCA (blue) and anti-proliferating cell nuclear (brown) antibodies. The right panel is magnification of the islet shown in the left column. (B) Fluorescent double staining of anti-PSCA antibody (red) and four islet-cell markers (blue), insulin (β cell), glucagon (α cell), somatostatin (δ cell) or pancreatic polypeptide (PP cell). Each series of the image was obtained by focusing on the islet-cell markers.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3472140&req=5

Figure 1: Prostate stem cell antigen (PSCA) is expressed in the islet of human normal pancreas (immunohistochemistry). (A) Double staining with anti-PSCA (blue) and anti-proliferating cell nuclear (brown) antibodies. The right panel is magnification of the islet shown in the left column. (B) Fluorescent double staining of anti-PSCA antibody (red) and four islet-cell markers (blue), insulin (β cell), glucagon (α cell), somatostatin (δ cell) or pancreatic polypeptide (PP cell). Each series of the image was obtained by focusing on the islet-cell markers.
Mentions: Embedded normal pancreas specimens on glass were purchased from BioChain Institute (Newark, CA, USA). Immunohistochemistry (Fig. 1A) was performed by the same procedure as described in the previous studies [4, 5] with mouse monoclonal anti-PSCA antibody whose specificity had been proved previously [4], rabbit anti-proliferating cell nuclear antigen (PCNA) antibody (sc-7907, Santa Cruz Biotechnology, Santa Cruz, CA, USA). The sections were incubated at 4℃ overnight with both antibodies simultaneously, and then with alkaline phosphatase conjugated anti-mouse IgG antibody and peroxidase conjugated anti-rabbit IgG antibody (NICHIREI Biosciences Inc., Tokyo, Japan) for an hour at room temperature. PSCA protein was visualized by Alkaline Phosphatase Substrate Kit III (Vector Laboratories, Burlingame, CA, USA) and PCNA by Vector NovaRED Substrate Kit for Peroxidase (Vector Laboratories). The fluorescent immunohistochemistry (Fig. 1B) was conducted using anti-PSCA, rabbit anti-insulin (sc-9186, Santa Cruz Biotechnology), rabbit anti-glucagon (ab18461, Abcam Japan, Tokyo, Japan), rabbit anti-somatostatin (A0566, Dako Japan, Tokyo, Japan) and goat anti-pancreatic polypeptide (NB100-1793, Novus Biologicals, Littleton, CO, USA) antibodies for primary antibodies, and anti-mouse IgG antibody-Rhodamin, anti-rabbit IgG-FITC and anti-goat IgG-FITC conjugates (sc-2300, sc-2090, sc-2024, respectively, Santa Cruz Biotechnology) for secondary antibodies. The fluorescent signals were obtained by Zeiss LSM510 META Confocal Imaging System (Carl Zeiss MicroImaging, LLC, Thornwood, NY, USA).

Bottom Line: Prostate stem cell antigen (PSCA) is a glycosylphosphatidylinositol-anchored cell surface antigen with an organ-dependent expression pattern in cancers; e.g., up-regulated in prostate cancer and down-regulated in gastric cancer.In our investigation of the transcription start site of PSCA, we found a non-coding splicing variant of PSCA as well as authentic PSCA transcripts in mRNA samples from a normal pancreas.We previously reported that PSCA expression is correlated to the methylation status of the enhancer region in gastric and gallbladder cancer cell lines but not in pancreatic cancer cell lines, suggesting that PSCA expression is regulated in a diff erent mode in pancreatic cancer from that in gastric and gallbladder cancers.

View Article: PubMed Central - PubMed

Affiliation: Division of Genetics, National Cancer Center Research Institute, Tokyo, Japan.

ABSTRACT
Prostate stem cell antigen (PSCA) is a glycosylphosphatidylinositol-anchored cell surface antigen with an organ-dependent expression pattern in cancers; e.g., up-regulated in prostate cancer and down-regulated in gastric cancer. Previously it was reported that PSCA is not expressed in the normal pancreas but aberrantly expressed in pancreatic cancer. In this present study, we identified PSCA expression in islets of the pancreas by immunohistochemistry, which was co-localized with four islet-cell markers: insulin, glucagon, somatostatin and pancreatic polypeptide. In our investigation of the transcription start site of PSCA, we found a non-coding splicing variant of PSCA as well as authentic PSCA transcripts in mRNA samples from a normal pancreas. Both the transcripts were also identified in several pancreatic cancer cell lines. We previously reported that PSCA expression is correlated to the methylation status of the enhancer region in gastric and gallbladder cancer cell lines but not in pancreatic cancer cell lines, suggesting that PSCA expression is regulated in a diff erent mode in pancreatic cancer from that in gastric and gallbladder cancers.

No MeSH data available.


Related in: MedlinePlus