Limits...
Distribution of lipid transfer protein 1 (LTP1) epitopes associated with morphogenic events during somatic embryogenesis of Arabidopsis thaliana.

Potocka I, Baldwin TC, Kurczynska EU - Plant Cell Rep. (2012)

Bottom Line: The labelling was strongest in both the outer periclinal and anticlinal walls of the adaxial, protodermal cells of the proximal region of the cotyledon.The putative role(s) of lipid transfer proteins in the formation of lipid lamellae and in cell differentiation are discussed.Key message Occurrence of lipid transfer protein 1 epitopes in Arabidopsis explant cells accompanies changes in cell fate and may be correlated with the deposition of lipid substances in the cell walls.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Cell Biology, University of Silesia, Jagiellońska 28, 40-032, Katowice, Poland. izabela.potocka@us.edu.pl

ABSTRACT
Using immunocytochemical methods, at both the light and electron microscopic level, we have investigated the spatial and temporal distribution of lipid transfer protein 1 (LTP1) epitopes during the induction of somatic embryogenesis in explants of Arabidopsis thaliana. Immunofluorescence labelling demonstrated the presence of high levels of LTP1 epitopes within the proximal regions of the cotyledons (embryogenic regions) associated with particular morphogenetic events, including intense cell division activity, cotyledon swelling, cell loosening and callus formation. Precise analysis of the signal localization in protodermal and subprotodermal cells indicated that cells exhibiting features typical of embryogenic cells were strongly labelled, both in walls and the cytoplasm, while in the majority of meristematic-like cells no signal was observed. Staining with lipophilic dyes revealed a correlation between the distribution of LTP1 epitopes and lipid substances within the cell wall. Differences in label abundance and distribution between embryogenic and non-embryogenic regions of explants were studied in detail with the use of immunogold electron microscopy. The labelling was strongest in both the outer periclinal and anticlinal walls of the adaxial, protodermal cells of the proximal region of the cotyledon. The putative role(s) of lipid transfer proteins in the formation of lipid lamellae and in cell differentiation are discussed. Key message Occurrence of lipid transfer protein 1 epitopes in Arabidopsis explant cells accompanies changes in cell fate and may be correlated with the deposition of lipid substances in the cell walls.

Show MeSH

Related in: MedlinePlus

Immunogold localization of LTP1 epitopes in the outer periclinal cell walls of protoderm from embryogenic (a–d) and non-embryogenic (e) regions of the explants. a, b The adaxial side of the proximal region of the cotyledons, day 6 of culture, labelling present throughout the wall, distributed uniformly (a) or in clusters (b, arrows). c, d The adaxial side of the proximal region of the cotyledons, day 7 (c) and day 9 (d) of culture, the labelling visible in both the outer and inner part of the cell wall (arrows), notice the undulated cell wall in c. e Shoot apex of an explant cultured for 6 days, weak label restricted to the outer part of the cell wall. f Negative control, no gold particles visible. ER endoplasmic reticulum. Scale bars 100 nm (a–f)
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3472069&req=5

Fig6: Immunogold localization of LTP1 epitopes in the outer periclinal cell walls of protoderm from embryogenic (a–d) and non-embryogenic (e) regions of the explants. a, b The adaxial side of the proximal region of the cotyledons, day 6 of culture, labelling present throughout the wall, distributed uniformly (a) or in clusters (b, arrows). c, d The adaxial side of the proximal region of the cotyledons, day 7 (c) and day 9 (d) of culture, the labelling visible in both the outer and inner part of the cell wall (arrows), notice the undulated cell wall in c. e Shoot apex of an explant cultured for 6 days, weak label restricted to the outer part of the cell wall. f Negative control, no gold particles visible. ER endoplasmic reticulum. Scale bars 100 nm (a–f)

Mentions: Localization of the LTP1 epitopes in the explant tissues was examined in more detail using immunogold labelling at the transmission electron microscope level. The TEM data demonstrated that the distribution of the LTP1 epitopes within the cell walls of embryogenic and non-embryogenic regions of the explants varied in terms of both spatial pattern and abundance. These differences were most clearly observed in both periclinal and anticlinal walls of protodermal cells (Figs. 6, 7, respectively).Fig. 6


Distribution of lipid transfer protein 1 (LTP1) epitopes associated with morphogenic events during somatic embryogenesis of Arabidopsis thaliana.

Potocka I, Baldwin TC, Kurczynska EU - Plant Cell Rep. (2012)

Immunogold localization of LTP1 epitopes in the outer periclinal cell walls of protoderm from embryogenic (a–d) and non-embryogenic (e) regions of the explants. a, b The adaxial side of the proximal region of the cotyledons, day 6 of culture, labelling present throughout the wall, distributed uniformly (a) or in clusters (b, arrows). c, d The adaxial side of the proximal region of the cotyledons, day 7 (c) and day 9 (d) of culture, the labelling visible in both the outer and inner part of the cell wall (arrows), notice the undulated cell wall in c. e Shoot apex of an explant cultured for 6 days, weak label restricted to the outer part of the cell wall. f Negative control, no gold particles visible. ER endoplasmic reticulum. Scale bars 100 nm (a–f)
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3472069&req=5

Fig6: Immunogold localization of LTP1 epitopes in the outer periclinal cell walls of protoderm from embryogenic (a–d) and non-embryogenic (e) regions of the explants. a, b The adaxial side of the proximal region of the cotyledons, day 6 of culture, labelling present throughout the wall, distributed uniformly (a) or in clusters (b, arrows). c, d The adaxial side of the proximal region of the cotyledons, day 7 (c) and day 9 (d) of culture, the labelling visible in both the outer and inner part of the cell wall (arrows), notice the undulated cell wall in c. e Shoot apex of an explant cultured for 6 days, weak label restricted to the outer part of the cell wall. f Negative control, no gold particles visible. ER endoplasmic reticulum. Scale bars 100 nm (a–f)
Mentions: Localization of the LTP1 epitopes in the explant tissues was examined in more detail using immunogold labelling at the transmission electron microscope level. The TEM data demonstrated that the distribution of the LTP1 epitopes within the cell walls of embryogenic and non-embryogenic regions of the explants varied in terms of both spatial pattern and abundance. These differences were most clearly observed in both periclinal and anticlinal walls of protodermal cells (Figs. 6, 7, respectively).Fig. 6

Bottom Line: The labelling was strongest in both the outer periclinal and anticlinal walls of the adaxial, protodermal cells of the proximal region of the cotyledon.The putative role(s) of lipid transfer proteins in the formation of lipid lamellae and in cell differentiation are discussed.Key message Occurrence of lipid transfer protein 1 epitopes in Arabidopsis explant cells accompanies changes in cell fate and may be correlated with the deposition of lipid substances in the cell walls.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Cell Biology, University of Silesia, Jagiellońska 28, 40-032, Katowice, Poland. izabela.potocka@us.edu.pl

ABSTRACT
Using immunocytochemical methods, at both the light and electron microscopic level, we have investigated the spatial and temporal distribution of lipid transfer protein 1 (LTP1) epitopes during the induction of somatic embryogenesis in explants of Arabidopsis thaliana. Immunofluorescence labelling demonstrated the presence of high levels of LTP1 epitopes within the proximal regions of the cotyledons (embryogenic regions) associated with particular morphogenetic events, including intense cell division activity, cotyledon swelling, cell loosening and callus formation. Precise analysis of the signal localization in protodermal and subprotodermal cells indicated that cells exhibiting features typical of embryogenic cells were strongly labelled, both in walls and the cytoplasm, while in the majority of meristematic-like cells no signal was observed. Staining with lipophilic dyes revealed a correlation between the distribution of LTP1 epitopes and lipid substances within the cell wall. Differences in label abundance and distribution between embryogenic and non-embryogenic regions of explants were studied in detail with the use of immunogold electron microscopy. The labelling was strongest in both the outer periclinal and anticlinal walls of the adaxial, protodermal cells of the proximal region of the cotyledon. The putative role(s) of lipid transfer proteins in the formation of lipid lamellae and in cell differentiation are discussed. Key message Occurrence of lipid transfer protein 1 epitopes in Arabidopsis explant cells accompanies changes in cell fate and may be correlated with the deposition of lipid substances in the cell walls.

Show MeSH
Related in: MedlinePlus