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Lactoferrin restrains allergen-induced pleurisy in mice.

Zimecki M, Artym J, Kocięba M, Kaleta-Kuratewicz K, Kruzel ML - Inflamm. Res. (2012)

Bottom Line: In particular, the percentages of eosinophils in blood and pleural exudates were strongly diminished.The histological analysis of lungs revealed that LF diminished the development of pathological lesions, such as pulmonary edema, diffuse alveolar hemorrhage and hemosiderosis, which were found in the lungs after injection of the eliciting dose of OVA.LF also decreased the level of IL-5 secreted into the pleural fluid.

View Article: PubMed Central - PubMed

Affiliation: Institute of Immunology and Experimental Therapy, Wrocław, Poland.

ABSTRACT

Objectives: The aim of this study was to assess the utility of lactoferrin (LF), a natural immunomodulator, to restrain allergen-induced pleurisy in mice.

Material and subjects: BALB/c female mice, 8- to 10-week old, weighing 24 g on average, were used.

Treatment: Mice were immunized intraperitoneally with 50 μg of ovalbumin (OVA) and the pleurisy was elicited 14 days later by intrapleural injection of 12.5 μg of OVA. LF was given 24 and 3 h before elicitation of the allergic reaction.

Methods: The cytokine levels in the pleural exudates were measured by immunoassays. The blood and pleural exudates smears were stained with Giemsa and May-Grünwald reagents and reviewed histologically. Lung sections were stained with eosin and hematoxylin for histological evaluation.

Results: Lactoferrin significantly decreased manifestation of pleurisy induced by OVA in a sensitized mouse model. In particular, the percentages of eosinophils in blood and pleural exudates were strongly diminished. The histological analysis of lungs revealed that LF diminished the development of pathological lesions, such as pulmonary edema, diffuse alveolar hemorrhage and hemosiderosis, which were found in the lungs after injection of the eliciting dose of OVA. LF also decreased the level of IL-5 secreted into the pleural fluid.

Conclusions: This is a first demonstration that LF significantly decreases antigen-specific pleurisy in a sensitized mouse model.

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Related in: MedlinePlus

Effect of LF on OVA-induced lung injury—histopathological changes. Mice were immunized with OVA and the allergic pleurisy reaction was elicited after 14 days as described in “Materials and methods”. Bovine LF (100 μg/dose) was given bucally to mice 24 and 3 h before elicitation of the allergic response. The histological analysis of lungs was performed 24 h later. a naive mice; b, c control, sensitized mice (OVA-sensitized mice which were given the eliciting dose of OVA and no LF); d mice treated with of BLF. a The lung of naive mouse (normal appearance); b severe edema and hemosiderin deposits in the lungs of sensitized control mouse; c alveolar hemorrhage and hemosiderosis in the lung of sensitized control mouse; d lung congestion of LF-treated mouse. Arrows indicate siderophages, H&E, original magnification× 400
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Fig6: Effect of LF on OVA-induced lung injury—histopathological changes. Mice were immunized with OVA and the allergic pleurisy reaction was elicited after 14 days as described in “Materials and methods”. Bovine LF (100 μg/dose) was given bucally to mice 24 and 3 h before elicitation of the allergic response. The histological analysis of lungs was performed 24 h later. a naive mice; b, c control, sensitized mice (OVA-sensitized mice which were given the eliciting dose of OVA and no LF); d mice treated with of BLF. a The lung of naive mouse (normal appearance); b severe edema and hemosiderin deposits in the lungs of sensitized control mouse; c alveolar hemorrhage and hemosiderosis in the lung of sensitized control mouse; d lung congestion of LF-treated mouse. Arrows indicate siderophages, H&E, original magnification× 400

Mentions: Histological analysis of OVA-sensitized mice revealed severe pulmonary edema (Fig. 6b). Edematous fluid was detected in the alveolar septa (interstitial pulmonary edema), within alveoli (alveolar pulmonary edema) and also within bronchioles. Edematous lesions were especially advanced in the subpleural tissue. Extensive interstitial and alveolar hemorrhages (Fig. 6c) were also observed. In the zones of extravasations the phenomenon of hemosiderosis was detected. That phenomenon consisted of appearance of hemosiderin-laden macrophages (siderophages) and hemosiderin deposits in the alveolar spaces and within alveolar septa. Moderate inflammatory infiltrations, formed by mononuclear cells around the bronchioles, blood vessels and in the interstitium, were also present.Fig. 6


Lactoferrin restrains allergen-induced pleurisy in mice.

Zimecki M, Artym J, Kocięba M, Kaleta-Kuratewicz K, Kruzel ML - Inflamm. Res. (2012)

Effect of LF on OVA-induced lung injury—histopathological changes. Mice were immunized with OVA and the allergic pleurisy reaction was elicited after 14 days as described in “Materials and methods”. Bovine LF (100 μg/dose) was given bucally to mice 24 and 3 h before elicitation of the allergic response. The histological analysis of lungs was performed 24 h later. a naive mice; b, c control, sensitized mice (OVA-sensitized mice which were given the eliciting dose of OVA and no LF); d mice treated with of BLF. a The lung of naive mouse (normal appearance); b severe edema and hemosiderin deposits in the lungs of sensitized control mouse; c alveolar hemorrhage and hemosiderosis in the lung of sensitized control mouse; d lung congestion of LF-treated mouse. Arrows indicate siderophages, H&E, original magnification× 400
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Related In: Results  -  Collection

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Fig6: Effect of LF on OVA-induced lung injury—histopathological changes. Mice were immunized with OVA and the allergic pleurisy reaction was elicited after 14 days as described in “Materials and methods”. Bovine LF (100 μg/dose) was given bucally to mice 24 and 3 h before elicitation of the allergic response. The histological analysis of lungs was performed 24 h later. a naive mice; b, c control, sensitized mice (OVA-sensitized mice which were given the eliciting dose of OVA and no LF); d mice treated with of BLF. a The lung of naive mouse (normal appearance); b severe edema and hemosiderin deposits in the lungs of sensitized control mouse; c alveolar hemorrhage and hemosiderosis in the lung of sensitized control mouse; d lung congestion of LF-treated mouse. Arrows indicate siderophages, H&E, original magnification× 400
Mentions: Histological analysis of OVA-sensitized mice revealed severe pulmonary edema (Fig. 6b). Edematous fluid was detected in the alveolar septa (interstitial pulmonary edema), within alveoli (alveolar pulmonary edema) and also within bronchioles. Edematous lesions were especially advanced in the subpleural tissue. Extensive interstitial and alveolar hemorrhages (Fig. 6c) were also observed. In the zones of extravasations the phenomenon of hemosiderosis was detected. That phenomenon consisted of appearance of hemosiderin-laden macrophages (siderophages) and hemosiderin deposits in the alveolar spaces and within alveolar septa. Moderate inflammatory infiltrations, formed by mononuclear cells around the bronchioles, blood vessels and in the interstitium, were also present.Fig. 6

Bottom Line: In particular, the percentages of eosinophils in blood and pleural exudates were strongly diminished.The histological analysis of lungs revealed that LF diminished the development of pathological lesions, such as pulmonary edema, diffuse alveolar hemorrhage and hemosiderosis, which were found in the lungs after injection of the eliciting dose of OVA.LF also decreased the level of IL-5 secreted into the pleural fluid.

View Article: PubMed Central - PubMed

Affiliation: Institute of Immunology and Experimental Therapy, Wrocław, Poland.

ABSTRACT

Objectives: The aim of this study was to assess the utility of lactoferrin (LF), a natural immunomodulator, to restrain allergen-induced pleurisy in mice.

Material and subjects: BALB/c female mice, 8- to 10-week old, weighing 24 g on average, were used.

Treatment: Mice were immunized intraperitoneally with 50 μg of ovalbumin (OVA) and the pleurisy was elicited 14 days later by intrapleural injection of 12.5 μg of OVA. LF was given 24 and 3 h before elicitation of the allergic reaction.

Methods: The cytokine levels in the pleural exudates were measured by immunoassays. The blood and pleural exudates smears were stained with Giemsa and May-Grünwald reagents and reviewed histologically. Lung sections were stained with eosin and hematoxylin for histological evaluation.

Results: Lactoferrin significantly decreased manifestation of pleurisy induced by OVA in a sensitized mouse model. In particular, the percentages of eosinophils in blood and pleural exudates were strongly diminished. The histological analysis of lungs revealed that LF diminished the development of pathological lesions, such as pulmonary edema, diffuse alveolar hemorrhage and hemosiderosis, which were found in the lungs after injection of the eliciting dose of OVA. LF also decreased the level of IL-5 secreted into the pleural fluid.

Conclusions: This is a first demonstration that LF significantly decreases antigen-specific pleurisy in a sensitized mouse model.

Show MeSH
Related in: MedlinePlus