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Lactoferrin restrains allergen-induced pleurisy in mice.

Zimecki M, Artym J, Kocięba M, Kaleta-Kuratewicz K, Kruzel ML - Inflamm. Res. (2012)

Bottom Line: In particular, the percentages of eosinophils in blood and pleural exudates were strongly diminished.The histological analysis of lungs revealed that LF diminished the development of pathological lesions, such as pulmonary edema, diffuse alveolar hemorrhage and hemosiderosis, which were found in the lungs after injection of the eliciting dose of OVA.LF also decreased the level of IL-5 secreted into the pleural fluid.

View Article: PubMed Central - PubMed

Affiliation: Institute of Immunology and Experimental Therapy, Wrocław, Poland.

ABSTRACT

Objectives: The aim of this study was to assess the utility of lactoferrin (LF), a natural immunomodulator, to restrain allergen-induced pleurisy in mice.

Material and subjects: BALB/c female mice, 8- to 10-week old, weighing 24 g on average, were used.

Treatment: Mice were immunized intraperitoneally with 50 μg of ovalbumin (OVA) and the pleurisy was elicited 14 days later by intrapleural injection of 12.5 μg of OVA. LF was given 24 and 3 h before elicitation of the allergic reaction.

Methods: The cytokine levels in the pleural exudates were measured by immunoassays. The blood and pleural exudates smears were stained with Giemsa and May-Grünwald reagents and reviewed histologically. Lung sections were stained with eosin and hematoxylin for histological evaluation.

Results: Lactoferrin significantly decreased manifestation of pleurisy induced by OVA in a sensitized mouse model. In particular, the percentages of eosinophils in blood and pleural exudates were strongly diminished. The histological analysis of lungs revealed that LF diminished the development of pathological lesions, such as pulmonary edema, diffuse alveolar hemorrhage and hemosiderosis, which were found in the lungs after injection of the eliciting dose of OVA. LF also decreased the level of IL-5 secreted into the pleural fluid.

Conclusions: This is a first demonstration that LF significantly decreases antigen-specific pleurisy in a sensitized mouse model.

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Related in: MedlinePlus

IL-5 (a) and IFN-γ (b) concentration in the pleural exudates. Mice were sensitized with OVA and the allergic pleurisy reaction was elicited after 14 days as described in “Materials and methods”. BLF (100 μg/dose) was given buccally to mice 24 and 3 h before OVA challenge. The pleural exudates were harvested 24 h later and the cytokine concentrations were determined by ELISA. The results were shown in picograms/ml of IL-5 or IFN-γ as mean values from 5 mice ± SE. Statistics: a BG versus control, P = 0.0001; control versus BLF, P = 0.0003 (ANOVA); b BG versus control NS (P = 0.6345); control versus BLF NS (P = 0.8510) (ANOVA). NS not significant
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Fig5: IL-5 (a) and IFN-γ (b) concentration in the pleural exudates. Mice were sensitized with OVA and the allergic pleurisy reaction was elicited after 14 days as described in “Materials and methods”. BLF (100 μg/dose) was given buccally to mice 24 and 3 h before OVA challenge. The pleural exudates were harvested 24 h later and the cytokine concentrations were determined by ELISA. The results were shown in picograms/ml of IL-5 or IFN-γ as mean values from 5 mice ± SE. Statistics: a BG versus control, P = 0.0001; control versus BLF, P = 0.0003 (ANOVA); b BG versus control NS (P = 0.6345); control versus BLF NS (P = 0.8510) (ANOVA). NS not significant

Mentions: Cytokines, in particular IL-5, are involved in mediation of the allergic inflammation. We found that IL-5 concentration, measured in the pleural fluid, was significantly elevated in sensitized mice as compared to the appropriate BG group (Fig. 5a). The pretreatment of mice with BLF (100 μg/mouse, buccally) reversed that phenomenon. On the other hand, IFN-γ levels were similar in sensitized and BG mice (Fig. 5b).Fig. 5


Lactoferrin restrains allergen-induced pleurisy in mice.

Zimecki M, Artym J, Kocięba M, Kaleta-Kuratewicz K, Kruzel ML - Inflamm. Res. (2012)

IL-5 (a) and IFN-γ (b) concentration in the pleural exudates. Mice were sensitized with OVA and the allergic pleurisy reaction was elicited after 14 days as described in “Materials and methods”. BLF (100 μg/dose) was given buccally to mice 24 and 3 h before OVA challenge. The pleural exudates were harvested 24 h later and the cytokine concentrations were determined by ELISA. The results were shown in picograms/ml of IL-5 or IFN-γ as mean values from 5 mice ± SE. Statistics: a BG versus control, P = 0.0001; control versus BLF, P = 0.0003 (ANOVA); b BG versus control NS (P = 0.6345); control versus BLF NS (P = 0.8510) (ANOVA). NS not significant
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3472062&req=5

Fig5: IL-5 (a) and IFN-γ (b) concentration in the pleural exudates. Mice were sensitized with OVA and the allergic pleurisy reaction was elicited after 14 days as described in “Materials and methods”. BLF (100 μg/dose) was given buccally to mice 24 and 3 h before OVA challenge. The pleural exudates were harvested 24 h later and the cytokine concentrations were determined by ELISA. The results were shown in picograms/ml of IL-5 or IFN-γ as mean values from 5 mice ± SE. Statistics: a BG versus control, P = 0.0001; control versus BLF, P = 0.0003 (ANOVA); b BG versus control NS (P = 0.6345); control versus BLF NS (P = 0.8510) (ANOVA). NS not significant
Mentions: Cytokines, in particular IL-5, are involved in mediation of the allergic inflammation. We found that IL-5 concentration, measured in the pleural fluid, was significantly elevated in sensitized mice as compared to the appropriate BG group (Fig. 5a). The pretreatment of mice with BLF (100 μg/mouse, buccally) reversed that phenomenon. On the other hand, IFN-γ levels were similar in sensitized and BG mice (Fig. 5b).Fig. 5

Bottom Line: In particular, the percentages of eosinophils in blood and pleural exudates were strongly diminished.The histological analysis of lungs revealed that LF diminished the development of pathological lesions, such as pulmonary edema, diffuse alveolar hemorrhage and hemosiderosis, which were found in the lungs after injection of the eliciting dose of OVA.LF also decreased the level of IL-5 secreted into the pleural fluid.

View Article: PubMed Central - PubMed

Affiliation: Institute of Immunology and Experimental Therapy, Wrocław, Poland.

ABSTRACT

Objectives: The aim of this study was to assess the utility of lactoferrin (LF), a natural immunomodulator, to restrain allergen-induced pleurisy in mice.

Material and subjects: BALB/c female mice, 8- to 10-week old, weighing 24 g on average, were used.

Treatment: Mice were immunized intraperitoneally with 50 μg of ovalbumin (OVA) and the pleurisy was elicited 14 days later by intrapleural injection of 12.5 μg of OVA. LF was given 24 and 3 h before elicitation of the allergic reaction.

Methods: The cytokine levels in the pleural exudates were measured by immunoassays. The blood and pleural exudates smears were stained with Giemsa and May-Grünwald reagents and reviewed histologically. Lung sections were stained with eosin and hematoxylin for histological evaluation.

Results: Lactoferrin significantly decreased manifestation of pleurisy induced by OVA in a sensitized mouse model. In particular, the percentages of eosinophils in blood and pleural exudates were strongly diminished. The histological analysis of lungs revealed that LF diminished the development of pathological lesions, such as pulmonary edema, diffuse alveolar hemorrhage and hemosiderosis, which were found in the lungs after injection of the eliciting dose of OVA. LF also decreased the level of IL-5 secreted into the pleural fluid.

Conclusions: This is a first demonstration that LF significantly decreases antigen-specific pleurisy in a sensitized mouse model.

Show MeSH
Related in: MedlinePlus