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Lactoferrin restrains allergen-induced pleurisy in mice.

Zimecki M, Artym J, Kocięba M, Kaleta-Kuratewicz K, Kruzel ML - Inflamm. Res. (2012)

Bottom Line: In particular, the percentages of eosinophils in blood and pleural exudates were strongly diminished.The histological analysis of lungs revealed that LF diminished the development of pathological lesions, such as pulmonary edema, diffuse alveolar hemorrhage and hemosiderosis, which were found in the lungs after injection of the eliciting dose of OVA.LF also decreased the level of IL-5 secreted into the pleural fluid.

View Article: PubMed Central - PubMed

Affiliation: Institute of Immunology and Experimental Therapy, Wrocław, Poland.

ABSTRACT

Objectives: The aim of this study was to assess the utility of lactoferrin (LF), a natural immunomodulator, to restrain allergen-induced pleurisy in mice.

Material and subjects: BALB/c female mice, 8- to 10-week old, weighing 24 g on average, were used.

Treatment: Mice were immunized intraperitoneally with 50 μg of ovalbumin (OVA) and the pleurisy was elicited 14 days later by intrapleural injection of 12.5 μg of OVA. LF was given 24 and 3 h before elicitation of the allergic reaction.

Methods: The cytokine levels in the pleural exudates were measured by immunoassays. The blood and pleural exudates smears were stained with Giemsa and May-Grünwald reagents and reviewed histologically. Lung sections were stained with eosin and hematoxylin for histological evaluation.

Results: Lactoferrin significantly decreased manifestation of pleurisy induced by OVA in a sensitized mouse model. In particular, the percentages of eosinophils in blood and pleural exudates were strongly diminished. The histological analysis of lungs revealed that LF diminished the development of pathological lesions, such as pulmonary edema, diffuse alveolar hemorrhage and hemosiderosis, which were found in the lungs after injection of the eliciting dose of OVA. LF also decreased the level of IL-5 secreted into the pleural fluid.

Conclusions: This is a first demonstration that LF significantly decreases antigen-specific pleurisy in a sensitized mouse model.

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Related in: MedlinePlus

The percentages of intact and de-granulated mastocytes in the pleural exudates. The experiment was performed as described in Fig. 3. 24 h after elicitation of the allergic response the percentages of intact and de-granulated mastocytes in the pleural exudates were determined. Statistics: de-granulated and intact mastocytes: BG versus control, P = 0.0001; control versus BLF 800, P = 0.0001; control versus BLF 400, P = 0.0001; control versus BLF 200, P = 0.0001; control versus BLF 100, P = 0.0001; control versus BLF 50, P = 0.0001; control versus dexameth., P = 0.0001 (ANOVA)
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Fig4: The percentages of intact and de-granulated mastocytes in the pleural exudates. The experiment was performed as described in Fig. 3. 24 h after elicitation of the allergic response the percentages of intact and de-granulated mastocytes in the pleural exudates were determined. Statistics: de-granulated and intact mastocytes: BG versus control, P = 0.0001; control versus BLF 800, P = 0.0001; control versus BLF 400, P = 0.0001; control versus BLF 200, P = 0.0001; control versus BLF 100, P = 0.0001; control versus BLF 50, P = 0.0001; control versus dexameth., P = 0.0001 (ANOVA)

Mentions: Significant differences were also observed in the proportions of intact and de-granulated mastocytes (Fig. 4). In control, sensitized mice, the percentage of de-granulated mastocytes was exceptionally high (82.4 %). LF or dexamethasone decreased the percentage of de-granulated mastocytes to the BG levels.Fig. 4


Lactoferrin restrains allergen-induced pleurisy in mice.

Zimecki M, Artym J, Kocięba M, Kaleta-Kuratewicz K, Kruzel ML - Inflamm. Res. (2012)

The percentages of intact and de-granulated mastocytes in the pleural exudates. The experiment was performed as described in Fig. 3. 24 h after elicitation of the allergic response the percentages of intact and de-granulated mastocytes in the pleural exudates were determined. Statistics: de-granulated and intact mastocytes: BG versus control, P = 0.0001; control versus BLF 800, P = 0.0001; control versus BLF 400, P = 0.0001; control versus BLF 200, P = 0.0001; control versus BLF 100, P = 0.0001; control versus BLF 50, P = 0.0001; control versus dexameth., P = 0.0001 (ANOVA)
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Related In: Results  -  Collection

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Fig4: The percentages of intact and de-granulated mastocytes in the pleural exudates. The experiment was performed as described in Fig. 3. 24 h after elicitation of the allergic response the percentages of intact and de-granulated mastocytes in the pleural exudates were determined. Statistics: de-granulated and intact mastocytes: BG versus control, P = 0.0001; control versus BLF 800, P = 0.0001; control versus BLF 400, P = 0.0001; control versus BLF 200, P = 0.0001; control versus BLF 100, P = 0.0001; control versus BLF 50, P = 0.0001; control versus dexameth., P = 0.0001 (ANOVA)
Mentions: Significant differences were also observed in the proportions of intact and de-granulated mastocytes (Fig. 4). In control, sensitized mice, the percentage of de-granulated mastocytes was exceptionally high (82.4 %). LF or dexamethasone decreased the percentage of de-granulated mastocytes to the BG levels.Fig. 4

Bottom Line: In particular, the percentages of eosinophils in blood and pleural exudates were strongly diminished.The histological analysis of lungs revealed that LF diminished the development of pathological lesions, such as pulmonary edema, diffuse alveolar hemorrhage and hemosiderosis, which were found in the lungs after injection of the eliciting dose of OVA.LF also decreased the level of IL-5 secreted into the pleural fluid.

View Article: PubMed Central - PubMed

Affiliation: Institute of Immunology and Experimental Therapy, Wrocław, Poland.

ABSTRACT

Objectives: The aim of this study was to assess the utility of lactoferrin (LF), a natural immunomodulator, to restrain allergen-induced pleurisy in mice.

Material and subjects: BALB/c female mice, 8- to 10-week old, weighing 24 g on average, were used.

Treatment: Mice were immunized intraperitoneally with 50 μg of ovalbumin (OVA) and the pleurisy was elicited 14 days later by intrapleural injection of 12.5 μg of OVA. LF was given 24 and 3 h before elicitation of the allergic reaction.

Methods: The cytokine levels in the pleural exudates were measured by immunoassays. The blood and pleural exudates smears were stained with Giemsa and May-Grünwald reagents and reviewed histologically. Lung sections were stained with eosin and hematoxylin for histological evaluation.

Results: Lactoferrin significantly decreased manifestation of pleurisy induced by OVA in a sensitized mouse model. In particular, the percentages of eosinophils in blood and pleural exudates were strongly diminished. The histological analysis of lungs revealed that LF diminished the development of pathological lesions, such as pulmonary edema, diffuse alveolar hemorrhage and hemosiderosis, which were found in the lungs after injection of the eliciting dose of OVA. LF also decreased the level of IL-5 secreted into the pleural fluid.

Conclusions: This is a first demonstration that LF significantly decreases antigen-specific pleurisy in a sensitized mouse model.

Show MeSH
Related in: MedlinePlus