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Lactoferrin restrains allergen-induced pleurisy in mice.

Zimecki M, Artym J, Kocięba M, Kaleta-Kuratewicz K, Kruzel ML - Inflamm. Res. (2012)

Bottom Line: In particular, the percentages of eosinophils in blood and pleural exudates were strongly diminished.The histological analysis of lungs revealed that LF diminished the development of pathological lesions, such as pulmonary edema, diffuse alveolar hemorrhage and hemosiderosis, which were found in the lungs after injection of the eliciting dose of OVA.LF also decreased the level of IL-5 secreted into the pleural fluid.

View Article: PubMed Central - PubMed

Affiliation: Institute of Immunology and Experimental Therapy, Wrocław, Poland.

ABSTRACT

Objectives: The aim of this study was to assess the utility of lactoferrin (LF), a natural immunomodulator, to restrain allergen-induced pleurisy in mice.

Material and subjects: BALB/c female mice, 8- to 10-week old, weighing 24 g on average, were used.

Treatment: Mice were immunized intraperitoneally with 50 μg of ovalbumin (OVA) and the pleurisy was elicited 14 days later by intrapleural injection of 12.5 μg of OVA. LF was given 24 and 3 h before elicitation of the allergic reaction.

Methods: The cytokine levels in the pleural exudates were measured by immunoassays. The blood and pleural exudates smears were stained with Giemsa and May-Grünwald reagents and reviewed histologically. Lung sections were stained with eosin and hematoxylin for histological evaluation.

Results: Lactoferrin significantly decreased manifestation of pleurisy induced by OVA in a sensitized mouse model. In particular, the percentages of eosinophils in blood and pleural exudates were strongly diminished. The histological analysis of lungs revealed that LF diminished the development of pathological lesions, such as pulmonary edema, diffuse alveolar hemorrhage and hemosiderosis, which were found in the lungs after injection of the eliciting dose of OVA. LF also decreased the level of IL-5 secreted into the pleural fluid.

Conclusions: This is a first demonstration that LF significantly decreases antigen-specific pleurisy in a sensitized mouse model.

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Related in: MedlinePlus

The cell number in the pleural exudates. Mice were immunized with OVA and the allergic pleurisy reaction was elicited after 14 days as described in “Materials and methods”. The mice were treated buccally with 800–50 μg doses of BLF at 24 and 3 h before the eliciting dose of antigen. Dexamethasone was used at a single dose of 20 μg, intraperitoneally, 3 h before elicitation of the allergic response. 24 h after elicitation of the response the numbers of cells in the pleural exudates were determined. The results were presented as the mean values from five mice/group ± SE. Statistics: BG versus control, P = 0.0001; control versus BLF 800, P = 0.0011; control versus BLF 400, P = 0.0001; control versus BLF 200, P = 0.0001; control versus BLF 100, P = 0.0001; control versus BLF 50, P = 0.0074; control versus dexameth., P = 0.0001 (ANOVA)
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Fig3: The cell number in the pleural exudates. Mice were immunized with OVA and the allergic pleurisy reaction was elicited after 14 days as described in “Materials and methods”. The mice were treated buccally with 800–50 μg doses of BLF at 24 and 3 h before the eliciting dose of antigen. Dexamethasone was used at a single dose of 20 μg, intraperitoneally, 3 h before elicitation of the allergic response. 24 h after elicitation of the response the numbers of cells in the pleural exudates were determined. The results were presented as the mean values from five mice/group ± SE. Statistics: BG versus control, P = 0.0001; control versus BLF 800, P = 0.0011; control versus BLF 400, P = 0.0001; control versus BLF 200, P = 0.0001; control versus BLF 100, P = 0.0001; control versus BLF 50, P = 0.0074; control versus dexameth., P = 0.0001 (ANOVA)

Mentions: Another experiment was aimed at establishing the optimal dose of LF using buccal administration of the protein. BLF was given at a dose range of 800–50 μg/mouse buccally and dexamethasone, a reference anti-inflammatory drug, at a single dose of 20 μg intraperitoneally, at 24 and 3 h before challenge with eliciting dose of OVA. Figure 3 shows a significant (almost a sixfold) increase of the cell numbers in the pleural exudates of the sensitized control mice over BG levels. The treatment of mice with decreasing doses of LF revealed an increasing, protective, anti-inflammatory effect with the optimal effect at 100 μg dose. The dose of 50 μg was much less effective. Therefore, in subsequent experiments the 100 μg dose of LF was used. The effect of dexamethasone was comparable to that of 100 μg of LF.Fig. 3


Lactoferrin restrains allergen-induced pleurisy in mice.

Zimecki M, Artym J, Kocięba M, Kaleta-Kuratewicz K, Kruzel ML - Inflamm. Res. (2012)

The cell number in the pleural exudates. Mice were immunized with OVA and the allergic pleurisy reaction was elicited after 14 days as described in “Materials and methods”. The mice were treated buccally with 800–50 μg doses of BLF at 24 and 3 h before the eliciting dose of antigen. Dexamethasone was used at a single dose of 20 μg, intraperitoneally, 3 h before elicitation of the allergic response. 24 h after elicitation of the response the numbers of cells in the pleural exudates were determined. The results were presented as the mean values from five mice/group ± SE. Statistics: BG versus control, P = 0.0001; control versus BLF 800, P = 0.0011; control versus BLF 400, P = 0.0001; control versus BLF 200, P = 0.0001; control versus BLF 100, P = 0.0001; control versus BLF 50, P = 0.0074; control versus dexameth., P = 0.0001 (ANOVA)
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Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3472062&req=5

Fig3: The cell number in the pleural exudates. Mice were immunized with OVA and the allergic pleurisy reaction was elicited after 14 days as described in “Materials and methods”. The mice were treated buccally with 800–50 μg doses of BLF at 24 and 3 h before the eliciting dose of antigen. Dexamethasone was used at a single dose of 20 μg, intraperitoneally, 3 h before elicitation of the allergic response. 24 h after elicitation of the response the numbers of cells in the pleural exudates were determined. The results were presented as the mean values from five mice/group ± SE. Statistics: BG versus control, P = 0.0001; control versus BLF 800, P = 0.0011; control versus BLF 400, P = 0.0001; control versus BLF 200, P = 0.0001; control versus BLF 100, P = 0.0001; control versus BLF 50, P = 0.0074; control versus dexameth., P = 0.0001 (ANOVA)
Mentions: Another experiment was aimed at establishing the optimal dose of LF using buccal administration of the protein. BLF was given at a dose range of 800–50 μg/mouse buccally and dexamethasone, a reference anti-inflammatory drug, at a single dose of 20 μg intraperitoneally, at 24 and 3 h before challenge with eliciting dose of OVA. Figure 3 shows a significant (almost a sixfold) increase of the cell numbers in the pleural exudates of the sensitized control mice over BG levels. The treatment of mice with decreasing doses of LF revealed an increasing, protective, anti-inflammatory effect with the optimal effect at 100 μg dose. The dose of 50 μg was much less effective. Therefore, in subsequent experiments the 100 μg dose of LF was used. The effect of dexamethasone was comparable to that of 100 μg of LF.Fig. 3

Bottom Line: In particular, the percentages of eosinophils in blood and pleural exudates were strongly diminished.The histological analysis of lungs revealed that LF diminished the development of pathological lesions, such as pulmonary edema, diffuse alveolar hemorrhage and hemosiderosis, which were found in the lungs after injection of the eliciting dose of OVA.LF also decreased the level of IL-5 secreted into the pleural fluid.

View Article: PubMed Central - PubMed

Affiliation: Institute of Immunology and Experimental Therapy, Wrocław, Poland.

ABSTRACT

Objectives: The aim of this study was to assess the utility of lactoferrin (LF), a natural immunomodulator, to restrain allergen-induced pleurisy in mice.

Material and subjects: BALB/c female mice, 8- to 10-week old, weighing 24 g on average, were used.

Treatment: Mice were immunized intraperitoneally with 50 μg of ovalbumin (OVA) and the pleurisy was elicited 14 days later by intrapleural injection of 12.5 μg of OVA. LF was given 24 and 3 h before elicitation of the allergic reaction.

Methods: The cytokine levels in the pleural exudates were measured by immunoassays. The blood and pleural exudates smears were stained with Giemsa and May-Grünwald reagents and reviewed histologically. Lung sections were stained with eosin and hematoxylin for histological evaluation.

Results: Lactoferrin significantly decreased manifestation of pleurisy induced by OVA in a sensitized mouse model. In particular, the percentages of eosinophils in blood and pleural exudates were strongly diminished. The histological analysis of lungs revealed that LF diminished the development of pathological lesions, such as pulmonary edema, diffuse alveolar hemorrhage and hemosiderosis, which were found in the lungs after injection of the eliciting dose of OVA. LF also decreased the level of IL-5 secreted into the pleural fluid.

Conclusions: This is a first demonstration that LF significantly decreases antigen-specific pleurisy in a sensitized mouse model.

Show MeSH
Related in: MedlinePlus