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Lactoferrin restrains allergen-induced pleurisy in mice.

Zimecki M, Artym J, Kocięba M, Kaleta-Kuratewicz K, Kruzel ML - Inflamm. Res. (2012)

Bottom Line: In particular, the percentages of eosinophils in blood and pleural exudates were strongly diminished.The histological analysis of lungs revealed that LF diminished the development of pathological lesions, such as pulmonary edema, diffuse alveolar hemorrhage and hemosiderosis, which were found in the lungs after injection of the eliciting dose of OVA.LF also decreased the level of IL-5 secreted into the pleural fluid.

View Article: PubMed Central - PubMed

Affiliation: Institute of Immunology and Experimental Therapy, Wrocław, Poland.

ABSTRACT

Objectives: The aim of this study was to assess the utility of lactoferrin (LF), a natural immunomodulator, to restrain allergen-induced pleurisy in mice.

Material and subjects: BALB/c female mice, 8- to 10-week old, weighing 24 g on average, were used.

Treatment: Mice were immunized intraperitoneally with 50 μg of ovalbumin (OVA) and the pleurisy was elicited 14 days later by intrapleural injection of 12.5 μg of OVA. LF was given 24 and 3 h before elicitation of the allergic reaction.

Methods: The cytokine levels in the pleural exudates were measured by immunoassays. The blood and pleural exudates smears were stained with Giemsa and May-Grünwald reagents and reviewed histologically. Lung sections were stained with eosin and hematoxylin for histological evaluation.

Results: Lactoferrin significantly decreased manifestation of pleurisy induced by OVA in a sensitized mouse model. In particular, the percentages of eosinophils in blood and pleural exudates were strongly diminished. The histological analysis of lungs revealed that LF diminished the development of pathological lesions, such as pulmonary edema, diffuse alveolar hemorrhage and hemosiderosis, which were found in the lungs after injection of the eliciting dose of OVA. LF also decreased the level of IL-5 secreted into the pleural fluid.

Conclusions: This is a first demonstration that LF significantly decreases antigen-specific pleurisy in a sensitized mouse model.

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Related in: MedlinePlus

The cell number in the pleural exudates. Mice were sensitized with OVA and the allergic reaction to OVA was elicited after 14 days as described in “Materials and methods”. Human or bovine LF was administered buccally (500 μg dose) at 24 and 3 h before the eliciting dose of antigen. The cell numbers in the pleural exudates were determined 24 h later. The results are presented as the mean values from five mice/group ± SE. Statistics: BG versus control, P = 0.0027; control versus BLF, P = 0.0002; control versus HLF, P = 0.0003 (ANOVA)
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Fig1: The cell number in the pleural exudates. Mice were sensitized with OVA and the allergic reaction to OVA was elicited after 14 days as described in “Materials and methods”. Human or bovine LF was administered buccally (500 μg dose) at 24 and 3 h before the eliciting dose of antigen. The cell numbers in the pleural exudates were determined 24 h later. The results are presented as the mean values from five mice/group ± SE. Statistics: BG versus control, P = 0.0027; control versus BLF, P = 0.0002; control versus HLF, P = 0.0003 (ANOVA)

Mentions: In a second experiment the effects of BLF and HLF, given buccally, were studied to assess the suppression of OVA-induced pleurisy. The results showed that although both LFs deeply suppressed the numbers of cells in the exudates (below BG levels), the action of bovine LF was stronger (Fig. 1). Likewise, BLF was slightly more effective in normalization of blood cell composition (Table 1) and number of de-granulated mastocytes in the pleural exudates (Fig. 2).Fig. 1


Lactoferrin restrains allergen-induced pleurisy in mice.

Zimecki M, Artym J, Kocięba M, Kaleta-Kuratewicz K, Kruzel ML - Inflamm. Res. (2012)

The cell number in the pleural exudates. Mice were sensitized with OVA and the allergic reaction to OVA was elicited after 14 days as described in “Materials and methods”. Human or bovine LF was administered buccally (500 μg dose) at 24 and 3 h before the eliciting dose of antigen. The cell numbers in the pleural exudates were determined 24 h later. The results are presented as the mean values from five mice/group ± SE. Statistics: BG versus control, P = 0.0027; control versus BLF, P = 0.0002; control versus HLF, P = 0.0003 (ANOVA)
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3472062&req=5

Fig1: The cell number in the pleural exudates. Mice were sensitized with OVA and the allergic reaction to OVA was elicited after 14 days as described in “Materials and methods”. Human or bovine LF was administered buccally (500 μg dose) at 24 and 3 h before the eliciting dose of antigen. The cell numbers in the pleural exudates were determined 24 h later. The results are presented as the mean values from five mice/group ± SE. Statistics: BG versus control, P = 0.0027; control versus BLF, P = 0.0002; control versus HLF, P = 0.0003 (ANOVA)
Mentions: In a second experiment the effects of BLF and HLF, given buccally, were studied to assess the suppression of OVA-induced pleurisy. The results showed that although both LFs deeply suppressed the numbers of cells in the exudates (below BG levels), the action of bovine LF was stronger (Fig. 1). Likewise, BLF was slightly more effective in normalization of blood cell composition (Table 1) and number of de-granulated mastocytes in the pleural exudates (Fig. 2).Fig. 1

Bottom Line: In particular, the percentages of eosinophils in blood and pleural exudates were strongly diminished.The histological analysis of lungs revealed that LF diminished the development of pathological lesions, such as pulmonary edema, diffuse alveolar hemorrhage and hemosiderosis, which were found in the lungs after injection of the eliciting dose of OVA.LF also decreased the level of IL-5 secreted into the pleural fluid.

View Article: PubMed Central - PubMed

Affiliation: Institute of Immunology and Experimental Therapy, Wrocław, Poland.

ABSTRACT

Objectives: The aim of this study was to assess the utility of lactoferrin (LF), a natural immunomodulator, to restrain allergen-induced pleurisy in mice.

Material and subjects: BALB/c female mice, 8- to 10-week old, weighing 24 g on average, were used.

Treatment: Mice were immunized intraperitoneally with 50 μg of ovalbumin (OVA) and the pleurisy was elicited 14 days later by intrapleural injection of 12.5 μg of OVA. LF was given 24 and 3 h before elicitation of the allergic reaction.

Methods: The cytokine levels in the pleural exudates were measured by immunoassays. The blood and pleural exudates smears were stained with Giemsa and May-Grünwald reagents and reviewed histologically. Lung sections were stained with eosin and hematoxylin for histological evaluation.

Results: Lactoferrin significantly decreased manifestation of pleurisy induced by OVA in a sensitized mouse model. In particular, the percentages of eosinophils in blood and pleural exudates were strongly diminished. The histological analysis of lungs revealed that LF diminished the development of pathological lesions, such as pulmonary edema, diffuse alveolar hemorrhage and hemosiderosis, which were found in the lungs after injection of the eliciting dose of OVA. LF also decreased the level of IL-5 secreted into the pleural fluid.

Conclusions: This is a first demonstration that LF significantly decreases antigen-specific pleurisy in a sensitized mouse model.

Show MeSH
Related in: MedlinePlus