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Quantitative trait loci associated with androgenic responsiveness in triticale (×Triticosecale Wittm.) anther culture.

Krzewska M, Czyczyło-Mysza I, Dubas E, Gołębiowska-Pikania G, Golemiec E, Stojałowski S, Chrupek M, Zur I - Plant Cell Rep. (2012)

Bottom Line: What is more, K-W test revealed additional markers on chromosomes: 5A, 2BL, 7B and 5R.Key message Five regions of the triticale genome were indicated as revealing significant marker/trait association.Markers located in these regions are potentially useful for triticale breeding through marker-assisted selection.

View Article: PubMed Central - PubMed

Affiliation: Institute of Plant Physiology, Polish Academy of Sciences, ul. Niezapominajek 21, 30-239, Kraków, Poland. monika.hara@gmail.com

ABSTRACT
Quantitative trait loci (QTLs) associated with androgenic responsiveness in triticale were analyzed using a population of 90 DH lines derived from the F1 cross between inbred line 'Saka 3006' and cv. 'Modus', which was used in a number of earlier studies on molecular mapping in this crop. Using Windows QTL Cartographer and MapQTL 5.0, composite interval mapping (CIM) and association studies (Kruskal-Wallis test; K-W) for five androgenesis parameters (androgenic embryo induction, total regeneration and green plant regeneration ability, and two characteristics describing final androgenesis efficiency) were conducted. For the studied components of androgenic response, CIM detected in total 28 QTLs which were localized on 5 chromosomes from A and R genomes. Effects of all QTLs that were identified at 2.0 or above of the LOD score explained 5.1-21.7 % of the phenotypic variation. Androgenesis induction was associated with seven QTLs (LOD between 2.0 and 5.8) detected on chromosomes 5A, 4R, 5R and 7R, all of them confirmed by K-W test as regions containing the markers significantly linked to the studied trait. What is more, K-W test revealed additional markers on chromosomes: 5A, 2BL, 7B and 5R. Both total and green regeneration ability were controlled by genes localized on chromosome 4A. Some of the QTLs that affected final androgenesis efficiency were identical with those associated with androgenic embryo induction efficiency, suggesting that the observed correlation may be either due to tight linkage or to pleiotropy. Key message Five regions of the triticale genome were indicated as revealing significant marker/trait association. Markers located in these regions are potentially useful for triticale breeding through marker-assisted selection.

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Related in: MedlinePlus

Chromosome maps showing locations of QTLs associated with androgenic responsiveness in anther culture of triticale (×Triticosecale Wittm.) Saka 3006/Modus mapping population. Results of Composite Interval Mapping for each studied traits: AS/100A (average number of androgenic structures per 100 anthers), GR/100AS (average number of green regenerants per 100 androgenic structures), R/100AS (average number of total regenerants per 100 androgenic structures), GR/100A (average number of green regenerants per 100 anthers), R/100A (average number of total regenerants per 100 anthers) in three replications. The ovals filled with color mean first replication, checkered ovals mean second replication and ovals with diagonal lines mean third replication of experiment (color version online)
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Fig1: Chromosome maps showing locations of QTLs associated with androgenic responsiveness in anther culture of triticale (×Triticosecale Wittm.) Saka 3006/Modus mapping population. Results of Composite Interval Mapping for each studied traits: AS/100A (average number of androgenic structures per 100 anthers), GR/100AS (average number of green regenerants per 100 androgenic structures), R/100AS (average number of total regenerants per 100 androgenic structures), GR/100A (average number of green regenerants per 100 anthers), R/100A (average number of total regenerants per 100 anthers) in three replications. The ovals filled with color mean first replication, checkered ovals mean second replication and ovals with diagonal lines mean third replication of experiment (color version online)

Mentions: Summarizing the results of CIM analysis from all three experimental replications, 28 QTLs were detected and localized on 5 chromosomes from A and R genomes for all studied components of androgenic response (Table 3; Fig. 1). Additionally, the analysis using Kruskal–Wallis test revealed some statistically significant markers (p < 0.005) located on chromosomes 2BL and 7B (Table 4).Table 3


Quantitative trait loci associated with androgenic responsiveness in triticale (×Triticosecale Wittm.) anther culture.

Krzewska M, Czyczyło-Mysza I, Dubas E, Gołębiowska-Pikania G, Golemiec E, Stojałowski S, Chrupek M, Zur I - Plant Cell Rep. (2012)

Chromosome maps showing locations of QTLs associated with androgenic responsiveness in anther culture of triticale (×Triticosecale Wittm.) Saka 3006/Modus mapping population. Results of Composite Interval Mapping for each studied traits: AS/100A (average number of androgenic structures per 100 anthers), GR/100AS (average number of green regenerants per 100 androgenic structures), R/100AS (average number of total regenerants per 100 androgenic structures), GR/100A (average number of green regenerants per 100 anthers), R/100A (average number of total regenerants per 100 anthers) in three replications. The ovals filled with color mean first replication, checkered ovals mean second replication and ovals with diagonal lines mean third replication of experiment (color version online)
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3472055&req=5

Fig1: Chromosome maps showing locations of QTLs associated with androgenic responsiveness in anther culture of triticale (×Triticosecale Wittm.) Saka 3006/Modus mapping population. Results of Composite Interval Mapping for each studied traits: AS/100A (average number of androgenic structures per 100 anthers), GR/100AS (average number of green regenerants per 100 androgenic structures), R/100AS (average number of total regenerants per 100 androgenic structures), GR/100A (average number of green regenerants per 100 anthers), R/100A (average number of total regenerants per 100 anthers) in three replications. The ovals filled with color mean first replication, checkered ovals mean second replication and ovals with diagonal lines mean third replication of experiment (color version online)
Mentions: Summarizing the results of CIM analysis from all three experimental replications, 28 QTLs were detected and localized on 5 chromosomes from A and R genomes for all studied components of androgenic response (Table 3; Fig. 1). Additionally, the analysis using Kruskal–Wallis test revealed some statistically significant markers (p < 0.005) located on chromosomes 2BL and 7B (Table 4).Table 3

Bottom Line: What is more, K-W test revealed additional markers on chromosomes: 5A, 2BL, 7B and 5R.Key message Five regions of the triticale genome were indicated as revealing significant marker/trait association.Markers located in these regions are potentially useful for triticale breeding through marker-assisted selection.

View Article: PubMed Central - PubMed

Affiliation: Institute of Plant Physiology, Polish Academy of Sciences, ul. Niezapominajek 21, 30-239, Kraków, Poland. monika.hara@gmail.com

ABSTRACT
Quantitative trait loci (QTLs) associated with androgenic responsiveness in triticale were analyzed using a population of 90 DH lines derived from the F1 cross between inbred line 'Saka 3006' and cv. 'Modus', which was used in a number of earlier studies on molecular mapping in this crop. Using Windows QTL Cartographer and MapQTL 5.0, composite interval mapping (CIM) and association studies (Kruskal-Wallis test; K-W) for five androgenesis parameters (androgenic embryo induction, total regeneration and green plant regeneration ability, and two characteristics describing final androgenesis efficiency) were conducted. For the studied components of androgenic response, CIM detected in total 28 QTLs which were localized on 5 chromosomes from A and R genomes. Effects of all QTLs that were identified at 2.0 or above of the LOD score explained 5.1-21.7 % of the phenotypic variation. Androgenesis induction was associated with seven QTLs (LOD between 2.0 and 5.8) detected on chromosomes 5A, 4R, 5R and 7R, all of them confirmed by K-W test as regions containing the markers significantly linked to the studied trait. What is more, K-W test revealed additional markers on chromosomes: 5A, 2BL, 7B and 5R. Both total and green regeneration ability were controlled by genes localized on chromosome 4A. Some of the QTLs that affected final androgenesis efficiency were identical with those associated with androgenic embryo induction efficiency, suggesting that the observed correlation may be either due to tight linkage or to pleiotropy. Key message Five regions of the triticale genome were indicated as revealing significant marker/trait association. Markers located in these regions are potentially useful for triticale breeding through marker-assisted selection.

Show MeSH
Related in: MedlinePlus