Calpain-2 expression is associated with response to platinum based chemotherapy, progression-free and overall survival in ovarian cancer.
Bottom Line: High levels of calpain-2 expression was significantly associated with platinum resistant tumours (P = 0.031).Furthermore, high expression of calpain-2 was significantly associated with progression-free (P = 0.049) and overall survival (P = 0.006) in this cohort.The association between calpain-2 expression and overall survival remained significant in multivariate analysis accounting for tumour grade, stage, optimal debulking and platinum sensitivity (hazard ratio = 2.174; 95% confidence interval = 1.144-4.130; P = 0.018).
Affiliation: Academic Oncology, University of Nottingham, School of Molecular Medical Sciences, Nottingham University Hospitals NHS Trust, Nottingham, UK.Show MeSH
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Mentions: Tissue expression of calpastatin, calpain-1 and calpain-2 was assessed in a series of 154 ovarian cancer patients treated with platinum-based chemotherapy. All three proteins were mainly located in the cytoplasm, with some granularity and heterogeneity between adjacent tumour cells, varying from weak to intense staining. Nuclear staining was observed quite frequently for calpastatin. Some stromal cell staining was observed, however, this was not scored as part of this study. Calpastatin had a mean H-score of 184 and a standard deviation of ±67; calpain-1 had a mean H-score of 114 ± 74 and calpain-2 had a mean H-score of 159 ± 47. Representative staining is shown in photomicrographs in Fig. 1. Two cores were assessed for each patient, with an average H-score being used for analysis. The correlation between the two scores for each patient was evaluated using Spearman's rank correlation coefficient. A significant correlation between the two scores for expression of calpain-1, calpain-2 and calpastatin was observed.
Affiliation: Academic Oncology, University of Nottingham, School of Molecular Medical Sciences, Nottingham University Hospitals NHS Trust, Nottingham, UK.