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Stepping stones in DNA sequencing.

Stranneheim H, Lundeberg J - Biotechnol J (2012)

Bottom Line: In recent years there have been tremendous advances in our ability to rapidly and cost-effectively sequence DNA.However, recent announcements in nanopore sequencing hold the promise of removing this read-length limitation, enabling sequencing of larger intact DNA fragments.This review covers some of the technical advances in sequencing that have opened up new frontiers in genomics.

View Article: PubMed Central - PubMed

Affiliation: Science for Life Laboratory, KTH Royal Institute of Technology, Stockholm, Sweden. henrik.stranneheim@scilifelab.se

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Related in: MedlinePlus

(A) Changes of read length and degree of parallelism in sequencing technologies since the 1990s up to the present. (B) Number of reads and read length per sequencing technology.
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fig01: (A) Changes of read length and degree of parallelism in sequencing technologies since the 1990s up to the present. (B) Number of reads and read length per sequencing technology.

Mentions: The most commonly used DNA sequencing technologies, and their challenges and limitations, are described below. A summary table of the features of each technology is provided in Table 1 while the number of sequence reads and read lengths are shown in Fig. 1.


Stepping stones in DNA sequencing.

Stranneheim H, Lundeberg J - Biotechnol J (2012)

(A) Changes of read length and degree of parallelism in sequencing technologies since the 1990s up to the present. (B) Number of reads and read length per sequencing technology.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3472021&req=5

fig01: (A) Changes of read length and degree of parallelism in sequencing technologies since the 1990s up to the present. (B) Number of reads and read length per sequencing technology.
Mentions: The most commonly used DNA sequencing technologies, and their challenges and limitations, are described below. A summary table of the features of each technology is provided in Table 1 while the number of sequence reads and read lengths are shown in Fig. 1.

Bottom Line: In recent years there have been tremendous advances in our ability to rapidly and cost-effectively sequence DNA.However, recent announcements in nanopore sequencing hold the promise of removing this read-length limitation, enabling sequencing of larger intact DNA fragments.This review covers some of the technical advances in sequencing that have opened up new frontiers in genomics.

View Article: PubMed Central - PubMed

Affiliation: Science for Life Laboratory, KTH Royal Institute of Technology, Stockholm, Sweden. henrik.stranneheim@scilifelab.se

Show MeSH
Related in: MedlinePlus