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Sexually dimorphic effects of a prenatal immune challenge on social play and vasopressin expression in juvenile rats.

Taylor PV, Veenema AH, Paul MJ, Bredewold R, Isaacs S, de Vries GJ - Biol Sex Differ (2012)

Bottom Line: These effects were not found in females.LPS treatment did not change AVP mRNA expression in the suprachiasmatic nucleus, paraventricular nucleus, or supraoptic nucleus of either sex, nor did it affect the sex difference in the size of the sexually dimorphic nucleus of the preoptic area.Given AVP's central role in regulating social behavior, the sexually dimorphic effects of prenatal LPS treatment on male AVP mRNA expression may contribute to the sexually dimorphic effect of LPS on male social play and may, therefore, increase understanding of factors that contribute to sex differences in social psychopathology.

View Article: PubMed Central - HTML - PubMed

Affiliation: Center for Neuroendocrine Studies and Department of Psychology, University of Massachusetts, Amherst, MA, 01003, USA. devries@cns.umass.edu.

ABSTRACT

Background: Infectious diseases and inflammation during pregnancy increase the offspring's risk for behavioral disorders. However, how immune stress affects neural circuitry during development is not well known. We tested whether a prenatal immune challenge interferes with the development of social play and with neural circuits implicated in social behavior.

Methods: Pregnant rats were given intraperitoneal injections of the bacterial endotoxin lipopolysaccharide (LPS - 100 μg /kg) or saline on the 15th day of pregnancy. Offspring were tested for social play behaviors between postnatal days 26-40. Brains were harvested on postnatal day 45 and processed for arginine vasopressin (AVP) mRNA in situ hybridization.

Results: In males, LPS treatment reduced the frequency of juvenile play behavior and reduced AVP mRNA expression in the medial amygdala and bed nucleus of the stria terminalis. These effects were not found in females. LPS treatment did not change AVP mRNA expression in the suprachiasmatic nucleus, paraventricular nucleus, or supraoptic nucleus of either sex, nor did it affect the sex difference in the size of the sexually dimorphic nucleus of the preoptic area.

Conclusions: Given AVP's central role in regulating social behavior, the sexually dimorphic effects of prenatal LPS treatment on male AVP mRNA expression may contribute to the sexually dimorphic effect of LPS on male social play and may, therefore, increase understanding of factors that contribute to sex differences in social psychopathology.

No MeSH data available.


Related in: MedlinePlus

Lack of effect of prenatal LPS on vasopressin expression in the SCN, PVN, and SON. Means (+ SEM) of the integrated density of AVP mRNA labeling in the SCN, PVN, and SON. Although there was no effect of LPS treatment, overall, males showed higher integrated density in the SON than did females (ANOVA, p < 0.008).
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Figure 4: Lack of effect of prenatal LPS on vasopressin expression in the SCN, PVN, and SON. Means (+ SEM) of the integrated density of AVP mRNA labeling in the SCN, PVN, and SON. Although there was no effect of LPS treatment, overall, males showed higher integrated density in the SON than did females (ANOVA, p < 0.008).

Mentions: In total, thirty brains were processed for AVP mRNA expression from male and female pups derived from three LPS-treated and three saline-treated litters. Due to poor histology some material could not be analyzed: 1 brain was excluded for the MeA, 4 for the BNST and SCN, 5 for the SON, and 6 for the PVN. As the data were averaged by litter, n = 3 per group was used for statistical analysis. Significant effects of LPS treatment on AVP mRNA expression were only found in the MeA and BST (Figure 2). Confirming the literature [22], juvenile males showed more AVP mRNA-expressing cells in the MeA and BST than females, (Figure 3; F(1,8) = 146.98, p < 0.0001; F(1,8) = 236.2, p < 0.0001 for MeA and BST, respectively). LPS treatment reduced AVP expression in males but not in females, thereby causing significant treatment X sex interactions in the MeA and BST (Figure 3; F(1,8) = 6.11, p < 0.04; F(1,8) = 7.45, p < 0.03 for MeA and BST, respectively). These sex-specific effects of LPS were restricted to the BST and MeA, as there were no LPS effects on AVP mRNA expression in the SON, PVN, or SCN (Figure 4). In accord with what has been reported for the size of the SON and its AVP neurons in 60-day old rats [23], we found that volume of the area expressing AVP mRNA and integrated density of AVP mRNA expression in the SON are larger in males than in females (Figure 4; F(1,8) = 12.57, p < 0.008 and F(1,8) = 12.86, p < 0.008 for volume and integrated density, respectively).


Sexually dimorphic effects of a prenatal immune challenge on social play and vasopressin expression in juvenile rats.

Taylor PV, Veenema AH, Paul MJ, Bredewold R, Isaacs S, de Vries GJ - Biol Sex Differ (2012)

Lack of effect of prenatal LPS on vasopressin expression in the SCN, PVN, and SON. Means (+ SEM) of the integrated density of AVP mRNA labeling in the SCN, PVN, and SON. Although there was no effect of LPS treatment, overall, males showed higher integrated density in the SON than did females (ANOVA, p < 0.008).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3420237&req=5

Figure 4: Lack of effect of prenatal LPS on vasopressin expression in the SCN, PVN, and SON. Means (+ SEM) of the integrated density of AVP mRNA labeling in the SCN, PVN, and SON. Although there was no effect of LPS treatment, overall, males showed higher integrated density in the SON than did females (ANOVA, p < 0.008).
Mentions: In total, thirty brains were processed for AVP mRNA expression from male and female pups derived from three LPS-treated and three saline-treated litters. Due to poor histology some material could not be analyzed: 1 brain was excluded for the MeA, 4 for the BNST and SCN, 5 for the SON, and 6 for the PVN. As the data were averaged by litter, n = 3 per group was used for statistical analysis. Significant effects of LPS treatment on AVP mRNA expression were only found in the MeA and BST (Figure 2). Confirming the literature [22], juvenile males showed more AVP mRNA-expressing cells in the MeA and BST than females, (Figure 3; F(1,8) = 146.98, p < 0.0001; F(1,8) = 236.2, p < 0.0001 for MeA and BST, respectively). LPS treatment reduced AVP expression in males but not in females, thereby causing significant treatment X sex interactions in the MeA and BST (Figure 3; F(1,8) = 6.11, p < 0.04; F(1,8) = 7.45, p < 0.03 for MeA and BST, respectively). These sex-specific effects of LPS were restricted to the BST and MeA, as there were no LPS effects on AVP mRNA expression in the SON, PVN, or SCN (Figure 4). In accord with what has been reported for the size of the SON and its AVP neurons in 60-day old rats [23], we found that volume of the area expressing AVP mRNA and integrated density of AVP mRNA expression in the SON are larger in males than in females (Figure 4; F(1,8) = 12.57, p < 0.008 and F(1,8) = 12.86, p < 0.008 for volume and integrated density, respectively).

Bottom Line: These effects were not found in females.LPS treatment did not change AVP mRNA expression in the suprachiasmatic nucleus, paraventricular nucleus, or supraoptic nucleus of either sex, nor did it affect the sex difference in the size of the sexually dimorphic nucleus of the preoptic area.Given AVP's central role in regulating social behavior, the sexually dimorphic effects of prenatal LPS treatment on male AVP mRNA expression may contribute to the sexually dimorphic effect of LPS on male social play and may, therefore, increase understanding of factors that contribute to sex differences in social psychopathology.

View Article: PubMed Central - HTML - PubMed

Affiliation: Center for Neuroendocrine Studies and Department of Psychology, University of Massachusetts, Amherst, MA, 01003, USA. devries@cns.umass.edu.

ABSTRACT

Background: Infectious diseases and inflammation during pregnancy increase the offspring's risk for behavioral disorders. However, how immune stress affects neural circuitry during development is not well known. We tested whether a prenatal immune challenge interferes with the development of social play and with neural circuits implicated in social behavior.

Methods: Pregnant rats were given intraperitoneal injections of the bacterial endotoxin lipopolysaccharide (LPS - 100 μg /kg) or saline on the 15th day of pregnancy. Offspring were tested for social play behaviors between postnatal days 26-40. Brains were harvested on postnatal day 45 and processed for arginine vasopressin (AVP) mRNA in situ hybridization.

Results: In males, LPS treatment reduced the frequency of juvenile play behavior and reduced AVP mRNA expression in the medial amygdala and bed nucleus of the stria terminalis. These effects were not found in females. LPS treatment did not change AVP mRNA expression in the suprachiasmatic nucleus, paraventricular nucleus, or supraoptic nucleus of either sex, nor did it affect the sex difference in the size of the sexually dimorphic nucleus of the preoptic area.

Conclusions: Given AVP's central role in regulating social behavior, the sexually dimorphic effects of prenatal LPS treatment on male AVP mRNA expression may contribute to the sexually dimorphic effect of LPS on male social play and may, therefore, increase understanding of factors that contribute to sex differences in social psychopathology.

No MeSH data available.


Related in: MedlinePlus