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Antioxidant sol-gel improves cutaneous wound healing in streptozotocin-induced diabetic rats.

Lee YH, Chang JJ, Chien CT, Yang MC, Chien HF - Exp Diabetes Res (2012)

Bottom Line: In vitro data showed that after 6 hours of air exposure, the order of the scavenging abilities for HOCl, H(2)O(2), and O(2) (-) was antioxidant sol-gel > antioxidant saline > Pluronic F127 = saline.After 7 and 14 days of wound injury, the antioxidant sol-gel improved wound healing significantly by accelerated epidermal and dermal maturation, an increase in collagen content, and a decrease in apoptosis formation.This study showed that Pluronic F127 plus vitamin C could potentially be employed as a novel wound-healing enhancer.

View Article: PubMed Central - PubMed

Affiliation: Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei 106, Taiwan.

ABSTRACT
We examined the effects of vitamin C in Pluronic F127 on diabetic wound healing. Full-thickness excision skin wounds were made in normal and diabetic Wistar rats to evaluate the effect of saline, saline plus vitamin C (antioxidant sol), Pluronic F127, or Pluronic F127 plus vitamin C (antioxidant sol-gel). The rate of wound contraction, the levels of epidermal and dermal maturation, collagen synthesis, and apoptosis production in the wound tissue were determined. In vitro data showed that after 6 hours of air exposure, the order of the scavenging abilities for HOCl, H(2)O(2), and O(2) (-) was antioxidant sol-gel > antioxidant saline > Pluronic F127 = saline. After 7 and 14 days of wound injury, the antioxidant sol-gel improved wound healing significantly by accelerated epidermal and dermal maturation, an increase in collagen content, and a decrease in apoptosis formation. However, the wounds of all treatments healed mostly at 3 weeks. Vitamin C in Pluronic F127 hastened cutaneous wound healing by its antioxidant and antiapoptotic mechanisms through a good drug delivery system. This study showed that Pluronic F127 plus vitamin C could potentially be employed as a novel wound-healing enhancer.

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Related in: MedlinePlus

Immunohistochemical evidence of keratinization by loricrin at 14th day. Wounds treated with saline control (a), Pluronic F127 sol (b), saline plus vitamin C (c), and Pluronic F127 plus vitamin C (d) were demonstrated. Original magnifications taken at ×100 and ×200 as inset. Immunohistochemical staining by antiloricrin (epidermal differentiation marker) antibodies showed that loricrin was highly expressed in the upper granular cell layer, especially in the regenerated epidermis of Pluronic F127 plus vitamin C or saline plus vitamin C groups.
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fig7: Immunohistochemical evidence of keratinization by loricrin at 14th day. Wounds treated with saline control (a), Pluronic F127 sol (b), saline plus vitamin C (c), and Pluronic F127 plus vitamin C (d) were demonstrated. Original magnifications taken at ×100 and ×200 as inset. Immunohistochemical staining by antiloricrin (epidermal differentiation marker) antibodies showed that loricrin was highly expressed in the upper granular cell layer, especially in the regenerated epidermis of Pluronic F127 plus vitamin C or saline plus vitamin C groups.

Mentions: With the help of hematoxylin & eosin stain (Figure 4) and the epidermal differentiation marker loricrin immunohistochemistry (Figure 7), the accelerated healing was noted in the epidermis of antioxidant saline or sol-gel-treated groups. Significant epidermal maturation indicated by migration of keratinization is indicated in an order of antioxidant sol-gel > antioxidant saline > Pluronic F127 > saline. Loricrin was abundant and finely granular in cells of the granular layer. The labeling was maximally present in the upper granular cells and suddenly decreased in the cornified layer, where a brick wall-like staining resulted.


Antioxidant sol-gel improves cutaneous wound healing in streptozotocin-induced diabetic rats.

Lee YH, Chang JJ, Chien CT, Yang MC, Chien HF - Exp Diabetes Res (2012)

Immunohistochemical evidence of keratinization by loricrin at 14th day. Wounds treated with saline control (a), Pluronic F127 sol (b), saline plus vitamin C (c), and Pluronic F127 plus vitamin C (d) were demonstrated. Original magnifications taken at ×100 and ×200 as inset. Immunohistochemical staining by antiloricrin (epidermal differentiation marker) antibodies showed that loricrin was highly expressed in the upper granular cell layer, especially in the regenerated epidermis of Pluronic F127 plus vitamin C or saline plus vitamin C groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3420222&req=5

fig7: Immunohistochemical evidence of keratinization by loricrin at 14th day. Wounds treated with saline control (a), Pluronic F127 sol (b), saline plus vitamin C (c), and Pluronic F127 plus vitamin C (d) were demonstrated. Original magnifications taken at ×100 and ×200 as inset. Immunohistochemical staining by antiloricrin (epidermal differentiation marker) antibodies showed that loricrin was highly expressed in the upper granular cell layer, especially in the regenerated epidermis of Pluronic F127 plus vitamin C or saline plus vitamin C groups.
Mentions: With the help of hematoxylin & eosin stain (Figure 4) and the epidermal differentiation marker loricrin immunohistochemistry (Figure 7), the accelerated healing was noted in the epidermis of antioxidant saline or sol-gel-treated groups. Significant epidermal maturation indicated by migration of keratinization is indicated in an order of antioxidant sol-gel > antioxidant saline > Pluronic F127 > saline. Loricrin was abundant and finely granular in cells of the granular layer. The labeling was maximally present in the upper granular cells and suddenly decreased in the cornified layer, where a brick wall-like staining resulted.

Bottom Line: In vitro data showed that after 6 hours of air exposure, the order of the scavenging abilities for HOCl, H(2)O(2), and O(2) (-) was antioxidant sol-gel > antioxidant saline > Pluronic F127 = saline.After 7 and 14 days of wound injury, the antioxidant sol-gel improved wound healing significantly by accelerated epidermal and dermal maturation, an increase in collagen content, and a decrease in apoptosis formation.This study showed that Pluronic F127 plus vitamin C could potentially be employed as a novel wound-healing enhancer.

View Article: PubMed Central - PubMed

Affiliation: Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei 106, Taiwan.

ABSTRACT
We examined the effects of vitamin C in Pluronic F127 on diabetic wound healing. Full-thickness excision skin wounds were made in normal and diabetic Wistar rats to evaluate the effect of saline, saline plus vitamin C (antioxidant sol), Pluronic F127, or Pluronic F127 plus vitamin C (antioxidant sol-gel). The rate of wound contraction, the levels of epidermal and dermal maturation, collagen synthesis, and apoptosis production in the wound tissue were determined. In vitro data showed that after 6 hours of air exposure, the order of the scavenging abilities for HOCl, H(2)O(2), and O(2) (-) was antioxidant sol-gel > antioxidant saline > Pluronic F127 = saline. After 7 and 14 days of wound injury, the antioxidant sol-gel improved wound healing significantly by accelerated epidermal and dermal maturation, an increase in collagen content, and a decrease in apoptosis formation. However, the wounds of all treatments healed mostly at 3 weeks. Vitamin C in Pluronic F127 hastened cutaneous wound healing by its antioxidant and antiapoptotic mechanisms through a good drug delivery system. This study showed that Pluronic F127 plus vitamin C could potentially be employed as a novel wound-healing enhancer.

Show MeSH
Related in: MedlinePlus